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Title: | Exploring the Diversity and Aromatic Hydrocarbon Degrading Potential of Epiphytic Fungi on Hornbeams from Chronically Polluted Areas | Authors: | IMPERATO, Valeria Portillo-Estrada, Miguel SARAN, Anabel Thoonen, Anneleen KOWALKOWSKI, Lukasz Gawronski, Stanislaw W. RINEAU, Francois VANGRONSVELD, Jaco THIJS, Sofie |
Issue Date: | 2021 | Publisher: | MDPI | Source: | JOURNAL OF FUNGI, 7 (11) , p. 972 (Art N° 972) | Abstract: | Plants can 'catch' and mitigate airborne pollutants and are assisted by fungi inhabiting their leaves. The structure and function of the fungal communities inhabiting the phyllosphere of hornbeam trees growing in two chronically polluted areas, the oilfield of Bobrka and the city center of Warsaw, were compared to the ones growing in one nature reserve, the Bialowieza National Park. Fungi were isolated and characterized both phylogenetically and functionally for their potential role in air pollution mitigation. Both culture-dependent (e.g., enzyme assays and tolerance tests) and culture-independent methods (e.g., ITS and shotgun sequencings) were used. Furthermore, the degradation potential of the fungi was assessed by gas chromatography mass spectrometry (GC-MS). Shotgun sequencing showed that the phyllosphere fungal communities were dominated by fungi belonging to the phylum Ascomycota. Aureobasidium was the only genus detected at the three locations with a relative abundance & GE;1.0%. Among the cultivated epiphytic fungi from Bobrka, Fusarium sporotrichioides AT11, Phoma herbarum AT15, and Lophiostoma sp. AT37 showed in vitro aromatic hydrocarbon degradation potential with laccase activities of 1.24, 3.62, and 7.2 mu U L-1, respectively, and peroxidase enzymes with activities of 3.46, 2.28, and 7.49 mu U L-1, respectively. Furthermore, Fusarium sporotrichioides AT11 and Phoma herbarum AT15 tolerated exposure to airborne naphthalene and benzene. Lophiostoma sp. AT37 was the most tolerant to exposure to these pollutants, in line with being the best potential aromatic hydrocarbon degrader isolated in this study. | Notes: | Imperato, V (corresponding author), Hasselt Univ, Ctr Environm Sci, Dept Biol, BE-3590 Diepenbeek, Belgium. miguel.portilloestrada@uantwerpen.be; saran.anabel@gmail.com; thoonen.anneleen@gmail.com; francois.rineau@uhasselt.be; jaco.vangronsveld@uhasselt.be; sofie.thijs@uhasselt.be |
Keywords: | air pollution; phyllosphere; phylloremediation; fungi | Document URI: | http://hdl.handle.net/1942/36264 | e-ISSN: | 2309-608X | DOI: | 10.3390/jof7110972 | ISI #: | WOS:000723785900001 | Rights: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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jof-07-00972-v2.pdf | Published version | 2.32 MB | Adobe PDF | View/Open |
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