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Title: | Advanced Glycation End Products Impair Cardiac Atrial Appendage Stem Cells Properties | Authors: | EVENS, Lize HEEREN, Ellen RUMMENS, Jean-Luc BRONCKAERS, Annelies HENDRIKX, Marc DELUYKER, Dorien BITO, Virginie |
Issue Date: | 2021 | Publisher: | MDPI | Source: | Journal of clinical medicine, 10 (13) (Art N° 2964) | Abstract: | Background: During myocardial infarction (MI), billions of cardiomyocytes are lost. The optimal therapy should effectively replace damaged cardiomyocytes, possibly with stem cells able to engraft and differentiate into adult functional cardiomyocytes. As such, cardiac atrial appendage stem cells (CASCs) are suitable candidates. However, the presence of elevated levels of advanced glycation end products (AGEs) in cardiac regions where CASCs are transplanted may affect their regenerative potential. In this study, we examine whether and how AGEs alter CASCs properties in vitro. Methods and Results: CASCs in culture were exposed to ranging AGEs concentrations (50 mu g/mL to 400 mu g/mL). CASCs survival, proliferation, and migration capacity were significantly decreased after 72 h of AGEs exposure. Apoptosis significantly increased with rising AGEs concentration. The harmful effects of these AGEs were partially blunted by pre-incubation with a receptor for AGEs (RAGE) inhibitor (25 mu M FPS-ZM1), indicating the involvement of RAGE in the observed negative effects. Conclusion: AGEs have a time- and concentration-dependent negative effect on CASCs survival, proliferation, migration, and apoptosis in vitro, partially mediated through RAGE activation. Whether anti-AGEs therapies are an effective treatment in the setting of stem cell therapy after MI warrants further examination. | Keywords: | stem cells;aldehyde dehydrogenase;CASCs;glycated proteins;advanced glycation end products;proliferation;apoptosis;migration;RAGE inhibition | Document URI: | http://hdl.handle.net/1942/36311 | e-ISSN: | 2077-0383 | DOI: | 10.3390/jcm10132964 | ISI #: | 000671401400001 | Rights: | 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2022 |
Appears in Collections: | Research publications |
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Advanced Glycation End Products Impair Cardiac Atrial Appendage Stem Cells Properties.pdf | Published version | 2.84 MB | Adobe PDF | View/Open |
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