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http://hdl.handle.net/1942/3640
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DC Field | Value | Language |
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dc.contributor.author | VANERUM, M | - |
dc.contributor.author | LEMMENS, Raf | - |
dc.contributor.author | Berden, J. | - |
dc.contributor.author | VANDUFFEL, Luc | - |
dc.contributor.author | TEUCHY, Henri | - |
dc.date.accessioned | 2007-11-29T09:55:21Z | - |
dc.date.available | 2007-11-29T09:55:21Z | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | EUROPEAN JOURNAL OF BIOCHEMISTRY, 227(1-2). p. 150-160 | - |
dc.identifier.issn | 0014-2956 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3640 | - |
dc.description.abstract | The protein responsible for the (Ca2+ or Mg2+)-ATPase activity in brush-border membranes from pig kidney tubular cells was characterized to distinguish this enzyme from the N-ethylmaleimide-sensitive Mg2+-ATPase, also present in renal brush borders. Both enzymes are clearly different in their pH optimum and their sensitivity to divalent cations, nucleoside 5'-triphosphates and inhibitors. Solubilization of the (Ca2+ or Mg2+)-ATPase from brush-border membrane vesicles was accomplished with Nonidet P-40 or dodecylmaltoside. However, simultaneous inactivation of the enzyme was inevitable. A tenfold enrichment of the ATPase activity was obtained by chromatofocusing of Nonidet-P-40-solubilized brush borders. A similar degree of purification was achieved by ion-exchange chromatography of dodecylmaltoside-solubilized preparations. From the SDS/polyacrylamide gels of partially purified (Ca2+ or Mg2+)-ATPase, a few protein bands could still be tentatively identified as responsible for the enzyme activity. Labeling of solubilized brush-border preparations with several radioactive ATP analogues also revealed that a protein band of molecular mass 90 kDa is the most probable candidate for the catalytic peptide of the (Ca2+ or Mg2+)-ATPase. Finally, immunoprecipitation as well as semi-dry blotting with antibodies generated against partially purified enzyme preparations, confirmed that a 90-kDa component is a reasonable candidate for the (Ca2+ or Mg2+)-ATPase in renal brush-border membranes. | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER VERLAG | - |
dc.subject.other | ATPASE, (CA2+ OR MG2+); ECTO-ATPASE; KIDNEY, BRUSH BORDER | - |
dc.title | Identification and partial purification of (Ca2+ or Mg2+)-ATPase in renal brushborder membranes | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 160 | - |
dc.identifier.issue | 1-2 | - |
dc.identifier.spage | 150 | - |
dc.identifier.volume | 227 | - |
local.format.pages | 11 | - |
dc.description.notes | LIMBURGS UNIV CENTRUM,DEPT MBW,BIOCHEM LAB,B-3590 DIEPENBEEK,BELGIUM. UNIV AMSTERDAM,EC SLATER INST BIOCHEM RES,AMSTERDAM,NETHERLANDS. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.isi | A1995QB85100018 | - |
dc.identifier.url | ttp://dx.doi.org/10.1111/j.1432-1033.1995.tb20371.x | - |
item.fulltext | No Fulltext | - |
item.fullcitation | VANERUM, M; LEMMENS, Raf; Berden, J.; VANDUFFEL, Luc & TEUCHY, Henri (1995) Identification and partial purification of (Ca2+ or Mg2+)-ATPase in renal brushborder membranes. In: EUROPEAN JOURNAL OF BIOCHEMISTRY, 227(1-2). p. 150-160. | - |
item.contributor | VANERUM, M | - |
item.contributor | LEMMENS, Raf | - |
item.contributor | Berden, J. | - |
item.contributor | VANDUFFEL, Luc | - |
item.contributor | TEUCHY, Henri | - |
item.accessRights | Closed Access | - |
Appears in Collections: | Research publications |
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