Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/36537
Title: | A Transcriptomic Atlas of the Ectomycorrhizal Fungus Laccaria bicolor | Authors: | RUYTINX, Joske Miyauchi, Shingo Hartmann-Wittulsky, Sebastian de Freitas Pereira, Maira Guinet, Frederic Churin, Jean-Louis PUT, Carine Le Tacon, Francois Veneault-Fourrey, Claire Martin, Francis Kohler, Annegret |
Issue Date: | 2021 | Publisher: | MDPI | Source: | Microorganisms, 9 (12) (Art N° 2612) | Abstract: | Trees are able to colonize, establish and survive in a wide range of soils through associations with ectomycorrhizal (EcM) fungi. Proper functioning of EcM fungi implies the differentiation of structures within the fungal colony. A symbiotic structure is dedicated to nutrient exchange and the extramatricular mycelium explores soil for nutrients. Eventually, basidiocarps develop to assure last stages of sexual reproduction. The aim of this study is to understand how an EcM fungus uses its gene set to support functional differentiation and development of specialized morphological structures. We examined the transcriptomes of Laccaria bicolor under a series of experimental setups, including the growth with Populus tremula x alba at different developmental stages, basidiocarps and free-living mycelium, under various conditions of N, P and C supply. In particular, N supply induced global transcriptional changes, whereas responses to P supply seemed to be independent from it. Symbiosis development with poplar is characterized by transcriptional waves. Basidiocarp development shares transcriptional signatures with other basidiomycetes. Overlaps in transcriptional responses of L. bicolor hyphae to a host plant and N/C supply next to co-regulation of genes in basidiocarps and mature mycorrhiza were detected. Few genes are induced in a single condition only, but functional and morphological differentiation rather involves fine tuning of larger gene sets. Overall, this transcriptomic atlas builds a reference to study the function and stability of EcM symbiosis in distinct conditions using L. bicolor as a model and indicates both similarities and differences with other ectomycorrhizal fungi, allowing researchers to distinguish conserved processes such as basidiocarp development from nutrient homeostasis. | Notes: | Ruytinx, J; Martin, F (corresponding author), Univ Lorraine, Inst Natl Rech Agr Alimentat & Environm INRAE, Interact Arbres Microorganismes, INRAE Grand Est Nancy,UMR1136, F-54280 Champenoux, France.; Ruytinx, J (corresponding author), Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Microbiol, B-1050 Brussels, Belgium.; Ruytinx, J (corresponding author), Vrije Univ Brussel, Dept Bioengn Sci, Res Grp Plant Genet, B-1050 Brussels, Belgium. joske.ruytinx@vub.be; smiyauchi@mpipz.mpg.de; sebastian.wittulsky@bayer.com; mairadefreitaspereira@gmail.com; frederic.guinet@univ-lorraine.fr; churin.jean-louis@orange.fr; carine.put@uhasselt.be; francois.letacon@gmail.com; claire.veneault-fourrey@inrae.fr; francis.martin@inrae.fr; annegret.kohler@inrae.fr |
Keywords: | ectomycorrhiza;symbiosis;carpophore;nitrogen;phosphorus;transcriptome | Document URI: | http://hdl.handle.net/1942/36537 | e-ISSN: | 2076-2607 | DOI: | 10.3390/microorganisms9122612 | ISI #: | WOS:000735532800001 | Rights: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
A Transcriptomic Atlas of the Ectomycorrhizal Fungus Laccaria bicolor.pdf | Published version | 3.42 MB | Adobe PDF | View/Open |
WEB OF SCIENCETM
Citations
13
checked on Oct 13, 2024
Page view(s)
26
checked on Sep 7, 2022
Download(s)
12
checked on Sep 7, 2022
Google ScholarTM
Check
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.