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http://hdl.handle.net/1942/3659
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DC Field | Value | Language |
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dc.contributor.author | LEYSSENS, Anne | - |
dc.contributor.author | VAN KERKHOVE, Emmy | - |
dc.contributor.author | ZHANG, SL | - |
dc.contributor.author | WELTENS, R | - |
dc.contributor.author | STEELS, Paul | - |
dc.date.accessioned | 2007-11-29T11:53:46Z | - |
dc.date.available | 2007-11-29T11:53:46Z | - |
dc.date.issued | 1993 | - |
dc.identifier.citation | JOURNAL OF INSECT PHYSIOLOGY, 39(11). p. 945-958 | - |
dc.identifier.issn | 0022-1910 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3659 | - |
dc.description.abstract | Cellular and luminal K+ concentrations were measured in different bath K+ concentrations using double-barrelled K+-selective electrodes. The electrochemical gradient for K+ across the basal and the apical cell membrane was estimated. The experiments were performed in two Cl- concentrations. In control Ringer (41 mM K+ and 57 mM Cl-) cell K+ was 82 mM and luminal K+ was 119 mM. On lowering bath K+ to 4 mM or increasing it to 90 mM cell K+ and to a lesser extent luminal K+ followed: cell K+ changed to 29 and 90 mM and luminal K+ to 106 and 132 mM, respectively. In a 143 mM Cl- Ringer solution cellular and luminal K+ concentration showed a similar change with the basal K+ concentration. The basal membrane potential difference was almost equal to the equilibrium potential for K+ in all bath K+ and Cl- concentrations tested and the estimated electrochemical gradient for K+ across the basal membrane was very small (inward, zero, or even outward in low bath K+). It tended to become more inward as the bath K+ increased. The methods for estimating such a small gradient and the idea of passive K+ uptake through K+ channels across this barrier was critically evaluated. The large transepithelial electrochemical gradient against which K+ was transported was primarily situated in the apical membrane. This gradient decreased as the bath K+ was elevated (and fluid secretion accelerated): an increase in the bath K+ reduced both the electrical and concentration step to be overcome for K+ secretion across the apical membrane. A double role for the bath K+ in the regulation of K+ secretion is proposed. | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject.other | MALPIGHIAN TUBULES; K+ TRANSPORT; ION-SELECTIVE MICROELECTRODES; TRANSMEMBRANE GRADIENTS | - |
dc.title | MEASUREMENT OF INTRACELLULAR AND LUMINAL K+ CONCENTRATIONS IN A MALPIGHIAN TUBULE (FORMICA) - ESTIMATE OF BASAL AND LUMINAL ELECTROCHEMICAL K+ GRADIENTS | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 958 | - |
dc.identifier.issue | 11 | - |
dc.identifier.spage | 945 | - |
dc.identifier.volume | 39 | - |
local.format.pages | 14 | - |
dc.description.notes | LIMBURGS UNIV CENTRUM,PHYSIOL LAB,B-3590 DIEPENBEEK,BELGIUM. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/0022-1910(93)90004-B | - |
dc.identifier.isi | A1993MK10700004 | - |
item.fulltext | No Fulltext | - |
item.fullcitation | LEYSSENS, Anne; VAN KERKHOVE, Emmy; ZHANG, SL; WELTENS, R & STEELS, Paul (1993) MEASUREMENT OF INTRACELLULAR AND LUMINAL K+ CONCENTRATIONS IN A MALPIGHIAN TUBULE (FORMICA) - ESTIMATE OF BASAL AND LUMINAL ELECTROCHEMICAL K+ GRADIENTS. In: JOURNAL OF INSECT PHYSIOLOGY, 39(11). p. 945-958. | - |
item.accessRights | Closed Access | - |
item.contributor | LEYSSENS, Anne | - |
item.contributor | VAN KERKHOVE, Emmy | - |
item.contributor | ZHANG, SL | - |
item.contributor | WELTENS, R | - |
item.contributor | STEELS, Paul | - |
Appears in Collections: | Research publications |
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