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Title: | The potential of bacteriophages to control Xanthomonas campestris pv. campestris at different stages of disease development | Authors: | Holtappels, Dominique Fortuna, Kiandro J. Moons, Lauren Broeckaert, Nand Backer, Leon E. Venneman, Sofie Rombouts, Sofie Lippens , Louis Baeyen , Steve Pollet, Sabien NOBEN, Jean-Paul Oechslin, Frank Vallino, Marta Aertsen, Abram Maes, Martine Van Vaerenbergh, Johan Lavigne, Rob Wagemans , Jeroen |
Issue Date: | 2022 | Publisher: | WILEY | Source: | Microbial Biotechnology, 15 (6) , p. 1762-1782 | Abstract: | Xanthomonas campestris pv. campestris (Xcc) is a vascular pathogen that invades the xylem of Brassica crops. Current chemical and antibiotics-based control measures for this bacterium are unsustainable and inefficient. After establishing a representative collection of Xcc strains, we isolated and characterized bacteriophages from two clades of phages to assess their potential in phage-based biocontrol. The most promising phages, FoX2 and FoX6, specifically recognize (lipo) polysaccharides, associated with the wxc gene cluster, on the surface of the bacterial cell wall. Next, we determined and optimized the applicability of FoX2 and FoX6 in an array of complementary bioassays, ranging from seed decontamination to irrigation- and spray-based applications. Here, an irrigation-based application showed promising results. In a final proof-of-concept, a CaCl2-formulated phage cocktail was shown to control the outbreak of Xcc in the open field. This comprehensive approach illustrates the potential of phage biocontrol of black rot disease in Brassica and serves as a reference for the broader implementation of phage biocontrol in integrated pest management strategies. | Notes: | Lavigne, R; Wagemans, J (corresponding author), Katholieke Univ Leuven, Lab Gene Technol, Leuven, Belgium. rob.lavigne@kuleuven.be; jeroen.wagemans@kuleuven.be |
Document URI: | http://hdl.handle.net/1942/36639 | ISSN: | 1751-7915 | e-ISSN: | 1751-7915 | DOI: | 10.1111/1751-7915.14004 | ISI #: | 000747858500001 | Rights: | 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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Microbial Biotechnology - 2022 - Holtappels - The potential of bacteriophages to control Xanthomonas campestris pv .pdf | Published version | 3.14 MB | Adobe PDF | View/Open |
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