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http://hdl.handle.net/1942/36952
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DC Field | Value | Language |
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dc.contributor.author | Hendrix , Hanne | - |
dc.contributor.author | Zimmermann-Kogadeeva, Maria | - |
dc.contributor.author | Zimmermann, Michael | - |
dc.contributor.author | Sauer, Uwe | - |
dc.contributor.author | De Smet , Jeroen | - |
dc.contributor.author | Muchez, Laurens | - |
dc.contributor.author | Lissens, Maries | - |
dc.contributor.author | Staes, Ines | - |
dc.contributor.author | Voet, Marleen | - |
dc.contributor.author | Wagemans , Jeroen | - |
dc.contributor.author | Ceyssens, Pieter-Jan | - |
dc.contributor.author | NOBEN, Jean-Paul | - |
dc.contributor.author | Aertsen, Abram | - |
dc.contributor.author | Lavigne, Rob | - |
dc.date.accessioned | 2022-03-21T13:27:15Z | - |
dc.date.available | 2022-03-21T13:27:15Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2022-03-18T09:35:59Z | - |
dc.identifier.citation | Cell Reports, 38 (7) (Art N° 110372) | - |
dc.identifier.uri | http://hdl.handle.net/1942/36952 | - |
dc.description.abstract | The Pseudomonas quinolone signal (PQS) is a multifunctional quorum sensing molecule of key importance to P. aeruginosa. Here, we report that the lytic Pseudomonas bacterial virus LUZ19 targets this population density-dependent signaling system by expressing quorum sensing targeting protein (Qst) early during infection. We demonstrate that Qst interacts with PqsD, a key host quinolone signal biosynthesis pathway enzyme, resulting in decreased levels of PQS and its precursor 2-heptyl-4(1H)-quinolone. The lack of a functional PqsD enzyme impairs LUZ19 infection but is restored by external supplementation of 2-heptyl-4(1H)-quinolone, suggesting that LUZ19 exploits the PQS system for successful infection. We establish a broad functional interaction network of Qst, which includes enzymes of cofactor biosynthesis pathways (CoaC/ThiD) and a non-ribosomal peptide synthetase pathway (PA1217). Qst therefore represents an exquisite example of intricate reprogramming of the bacterium by a phage, which may be further exploited as tool to combat antibiotic resistant bacterial pathogens. | - |
dc.description.sponsorship | We thank Prof. Dirk De Vos (Center for Surface Chemistry and Catalysis, KU Leuven, Belgium) for use of the NMR facilities and Erik Royackers (Hasselt University, Belgium) for the technical support in mass spectrometry analysis. This research was supported by the KU Leuven project GOA ‘‘Phage Biosystems’’ (GOA/15/006) and the C1 project ‘ACES’ (C16/20/001). | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.rights | 2022 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.title | Metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 7 | - |
dc.identifier.volume | 38 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Lavigne, R (corresponding author), Katholieke Univ Leuven, Dept Biosyst, Lab Gene Technol, B-3001 Heverlee, Belgium. | - |
dc.description.notes | rob.lavigne@kuleuven.be | - |
local.publisher.place | 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 110372 | - |
dc.identifier.doi | 10.1016/j.celrep.2022.110372 | - |
dc.identifier.isi | WOS:000759253300004 | - |
dc.contributor.orcid | Aertsen, Abram/0000-0002-1897-2305; Wagemans, Jeroen/0000-0002-2185-5724 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Hendrix, Hanne; De Smet, Jeroen; Lissens, Maries; Voet, Marleen; Wagemans, Jeroen; Ceyssens, Pieter-Jan; Lavigne, Rob] Katholieke Univ Leuven, Dept Biosyst, Lab Gene Technol, B-3001 Heverlee, Belgium. | - |
local.description.affiliation | [Zimmermann-Kogadeeva, Maria; Zimmermann, Michael; Sauer, Uwe] Swiss Fed Inst Technol, Inst Mol Syst Biol, CH-8092 Zueurorich, Switzerland. | - |
local.description.affiliation | [Muchez, Laurens] Katholieke Univ Leuven, Ctr Surface Chem & Catalysis, Dept Microbial & Mol Syst, B-3001 Heverlee, Belgium. | - |
local.description.affiliation | [Staes, Ines; Aertsen, Abram] Katholieke Univ Leuven, Dept Microbial & Mol Syst, Lab Food Microbiol, B-3001 Heverlee, Belgium. | - |
local.description.affiliation | [Noben, Jean-Paul] Hasselt Univ, Biomed Res Inst, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Noben, Jean-Paul] Hasselt Univ, Sch Life Sci, Transnat Univ Limburg, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Zimmermann-Kogadeeva, Maria] European Mol Biol Lab, Genome Biol Unit, D-69117 Heidelberg, Germany. | - |
local.description.affiliation | [Zimmermann, Michael] European Mol Biol Lab, Struct & Computat Biol Unit, D-69117 Heidelberg, Germany. | - |
local.description.affiliation | [De Smet, Jeroen] Katholieke Univ Leuven, Dept Microbial & Mol Syst, Lab4Food, B-2240 Geel, Belgium. | - |
local.description.affiliation | [Ceyssens, Pieter-Jan] Sciensano, Unit Human Bacterial Dis, B-1050 Brussels, Belgium. | - |
local.uhasselt.international | yes | - |
item.validation | ecoom 2023 | - |
item.contributor | Hendrix , Hanne | - |
item.contributor | Zimmermann-Kogadeeva, Maria | - |
item.contributor | Zimmermann, Michael | - |
item.contributor | Sauer, Uwe | - |
item.contributor | De Smet , Jeroen | - |
item.contributor | Muchez, Laurens | - |
item.contributor | Lissens, Maries | - |
item.contributor | Staes, Ines | - |
item.contributor | Voet, Marleen | - |
item.contributor | Wagemans , Jeroen | - |
item.contributor | Ceyssens, Pieter-Jan | - |
item.contributor | NOBEN, Jean-Paul | - |
item.contributor | Aertsen, Abram | - |
item.contributor | Lavigne, Rob | - |
item.accessRights | Open Access | - |
item.fullcitation | Hendrix , Hanne; Zimmermann-Kogadeeva, Maria; Zimmermann, Michael; Sauer, Uwe; De Smet , Jeroen; Muchez, Laurens; Lissens, Maries; Staes, Ines; Voet, Marleen; Wagemans , Jeroen; Ceyssens, Pieter-Jan; NOBEN, Jean-Paul; Aertsen, Abram & Lavigne, Rob (2022) Metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation. In: Cell Reports, 38 (7) (Art N° 110372). | - |
item.fulltext | With Fulltext | - |
crisitem.journal.issn | 2211-1247 | - |
crisitem.journal.eissn | 2211-1247 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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Metabolic reprogramming of Pseudomonas aeruginosa by phage-based quorum sensing modulation.pdf | Published version | 3.04 MB | Adobe PDF | View/Open |
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