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http://hdl.handle.net/1942/36977
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DC Field | Value | Language |
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dc.contributor.author | Vitale, M. R. | - |
dc.contributor.author | Zoeller, J. E. M. | - |
dc.contributor.author | Ziegler, G. C. | - |
dc.contributor.author | Von den Hove, D. | - |
dc.contributor.author | VANMIERLO, Tim | - |
dc.contributor.author | Lesch, K. P. | - |
dc.date.accessioned | 2022-03-24T12:00:17Z | - |
dc.date.available | 2022-03-24T12:00:17Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2022-03-04T15:17:14Z | - |
dc.identifier.citation | EUROPEAN NEUROPSYCHOPHARMACOLOGY, 53 , p. S190 -S191 | - |
dc.identifier.uri | http://hdl.handle.net/1942/36977 | - |
dc.description.abstract | s man IL-6 or vehicle for either 3 or 24 hours, RNA and media samples were collected. Protein samples for immunoblot-ting were collected at 15, 30, 60 and 180 minutes after IL-6 or vehicle stimulation. For secretome analysis, media samples were processed with the Bio-Techne Proteome Profiler Kit. Confirming a microglial-like phenotype, qPCR revealed increased expression of microglia signature genes MERTK (p = 0.0012 1way ANOVA) and P2RY12 (p < 0.0001; 1way-ANOVA) with longer differentiation from MGL-progenitors to mature MGLs. Receptor transcripts required for IL-6 signalling (p = 0.0009; IL6ST, p = 0.0046; 1way-ANOVA) also increased with MGL differentiation. ICC confirmed > 95% of MGL-progenitors and mature MGLs expressed both PU.1 and TMEM119 proteins. Moreover, soma size (p = 8.275x10-5) and arborization (p = 0.0022) area increased during differentiation from MGL-progenitors to mature MGLs. Exposure to IL-6 resulted in time-dependent increases in Y705-STAT-3 phos-phorylation in mature MGLs peaking at 15-30mins after IL-6 stimulation confirming activation. MGLs responded to IL-6 by increasing IL-6 expression itself (Treatment p = 0.0196; 2way-ANOVA) and the IL-6 induced secretome consisted of an efflux of specific chemokines, including MIF, MIP-1A/1B, CCL1/2, Serpin-E1 and GROa, with maximal effects observed 24 hours post-exposure. Collectively, these data confirm we can recapitulate a human mature microglia-like cell in monoculture in line with prior data [5]. These cells express the necessary receptor machinery for IL-6 signalling and IL-6 exposure induces both STAT-3 phosphorylation and increased secretion of IL-6 and pro-inflammatory chemokines. These data provide a foundation for future studies in vitro using co-cultures of hiPSC-derived MGLs with NPCs using patient derived material. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.rights | 2021 Published by Elsevier B.V. | - |
dc.title | P.0261 Investigation of cdh13’s role in neurodevelopmental disorders using isogenic induced pluripotent stem cell (ipsc) lines and different cdh13 snp variants | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | S191 | - |
dc.identifier.spage | S190 | - |
dc.identifier.volume | 53 | - |
local.format.pages | 2 | - |
local.bibliographicCitation.jcat | M | - |
local.publisher.place | RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS | - |
local.type.refereed | Refereed | - |
local.type.specified | Meeting Abstract | - |
local.class | dsPublValOverrule/author_version_not_expected | - |
dc.identifier.doi | 10.1016/j.euroneuro.2021.10.251 | - |
dc.identifier.isi | WOS:000753359500241 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Vitale, M. R.; Zoeller, J. E. M.; Ziegler, G. C.; Von den Hove, D.; Lesch, K. P.] Univ Hosp Wurzburg Germany, Ctr Mental Hlth, Div Mol Psychiat, Wurzburg, Germany. | - |
local.description.affiliation | [Vitale, M. R.; Lesch, K. P.] IM Sechenov First Moscow State Med Univ, Inst Mol Med, Moscow, Russia. | - |
local.description.affiliation | [Vitale, M. R.; Lesch, K. P.] Lab Psychiat Neurobiol, Moscow, Russia. | - |
local.description.affiliation | [Zoeller, J. E. M.; Lesch, K. P.] Maastricht Univ, Sch Mental Hlth & Neurosci MHeNs, Maastricht, Netherlands. | - |
local.description.affiliation | [Zoeller, J. E. M.; Lesch, K. P.] Dept Psychiat & Neuropsychol, Maastricht, Netherlands. | - |
local.description.affiliation | [Ziegler, G. C.; Von den Hove, D.] Univ Hosp Wurzburg, Ctr Mental Hlth, Wurzburg, Germany. | - |
local.description.affiliation | [Ziegler, G. C.] Univ Hosp Wurzburg Germany, Ctr Mental Hlth, Dept Psychiatry Psychosomat & Psychotherapy, Wurzburg, Germany. | - |
local.description.affiliation | [Von den Hove, D.] Maastricht Univ, Sch Mental Hlth & Neurosci MHeNs, Maastricht, Netherlands. | - |
local.description.affiliation | [Von den Hove, D.] Dept Psychiat & Neuropsychol, Maastricht, Netherlands. | - |
local.description.affiliation | [Vanmierlo, T.] Hasselt Univ Belgium, Biomed Res Inst, Neuroimmune Connect & Repair Lab, Hasselt, Belgium. | - |
local.uhasselt.international | yes | - |
item.contributor | Vitale, M. R. | - |
item.contributor | Zoeller, J. E. M. | - |
item.contributor | Ziegler, G. C. | - |
item.contributor | Von den Hove, D. | - |
item.contributor | VANMIERLO, Tim | - |
item.contributor | Lesch, K. P. | - |
item.fulltext | With Fulltext | - |
item.fullcitation | Vitale, M. R.; Zoeller, J. E. M.; Ziegler, G. C.; Von den Hove, D.; VANMIERLO, Tim & Lesch, K. P. (2021) P.0261 Investigation of cdh13’s role in neurodevelopmental disorders using isogenic induced pluripotent stem cell (ipsc) lines and different cdh13 snp variants. In: EUROPEAN NEUROPSYCHOPHARMACOLOGY, 53 , p. S190 -S191. | - |
item.accessRights | Restricted Access | - |
crisitem.journal.issn | 0924-977X | - |
crisitem.journal.eissn | 1873-7862 | - |
Appears in Collections: | Research publications |
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P.0261 Investigation of cdh13’s role in neurodevelopmental disorders using isogenic induced pluripotent stem cell (ipsc) lines and different cdh13 snp variants.pdf Restricted Access | Published version | 67.35 kB | Adobe PDF | View/Open Request a copy |
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