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http://hdl.handle.net/1942/37038
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DC Field | Value | Language |
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dc.contributor.author | Sveinsdottir, Hildur Soley | - |
dc.contributor.author | Decker , Amanda | - |
dc.contributor.author | Christensen, Christian | - |
dc.contributor.author | BOTELLA LUCENA, Pablo | - |
dc.contributor.author | Porsteinsson, Haraldur | - |
dc.contributor.author | Richert, Elena | - |
dc.contributor.author | Maier, Valerie Helene | - |
dc.contributor.author | Cornell, Robert | - |
dc.contributor.author | Karlsson, Karl Aegir | - |
dc.contributor.editor | Neuhauss, Stephan C.F. | - |
dc.date.accessioned | 2022-03-28T12:26:38Z | - |
dc.date.available | 2022-03-28T12:26:38Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2022-03-25T12:34:08Z | - |
dc.identifier.citation | PLoS One, 17 (1) (Art N° e0259753) | - |
dc.identifier.uri | http://hdl.handle.net/1942/37038 | - |
dc.description.abstract | In the present study, we characterize a novel zebrafish mutant of solute carrier 18A2 (slc18a2), also known as vesicular monoamine transporter 2 (vmat2), that exhibits a behavioural phenotype partially consistent with human Parkinson's disease. At six days-post-fertilization, behaviour was analysed and demonstrated that vmat2 homozygous mutant larvae, relative to wild types, show changes in motility in a photomotor assay, altered sleep parameters, and reduced dopamine cell number. Following an abrupt lights-off stimulus mutant larvae initiate larger movements but subsequently inhibit them to a lesser extent in comparison to wild-type larvae. Conversely, during a lights-on period, the mutant larvae are hypomotile. Thigmotaxis, a preference to avoid the centre of a behavioural arena, was increased in homozygotes over heterozygotes and wild types, as was daytime sleep ratio. Furthermore, incubating mutant larvae in pramipexole or L-Dopa partially rescued the motor phenotypes, as did injecting glial cell-derived neurotrophic factor (GDNF) into their brains. This novel vmat2 model represents a tool for high throughput pharmaceutical screens for novel therapeutics, in particular those that increase monoamine transport, and for studies of the function of monoamine transporters. | - |
dc.description.sponsorship | This work is funded in part by grants from the National Institutes of Health GM067841 (RAC) and AR062547 (RAC). NO The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.rights | 2022 Sveinsdo´ttir et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.subject.other | Animals | - |
dc.subject.other | Brain | - |
dc.subject.other | Dopamine | - |
dc.subject.other | Dopamine Plasma Membrane Transport Proteins | - |
dc.subject.other | Glial Cell Line-Derived Neurotrophic Factor | - |
dc.subject.other | Locomotion | - |
dc.subject.other | Vesicular Monoamine Transport Proteins | - |
dc.subject.other | Zebrafish | - |
dc.subject.other | Zebrafish Proteins | - |
dc.title | Motility phenotype in a zebrafish vmat2 mutant | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 1 | - |
dc.identifier.volume | 17 | - |
local.format.pages | 19 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Karlsson, KÆ (corresponding author), 3Z, Reykjavik, Iceland.; Karlsson, KÆ (corresponding author), Reykjavik Univ, Sch Sci & Engn, Reykjavik, Iceland.; Karlsson, KÆ (corresponding author), Univ Iceland, Biomed Ctr, Reykjavik, Iceland. | - |
dc.description.notes | karlsson@ru.is | - |
local.publisher.place | 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | e0259753 | - |
dc.identifier.doi | 10.1371/journal.pone.0259753 | - |
dc.identifier.pmid | 34986152 | - |
dc.identifier.isi | WOS:000765956200005 | - |
dc.contributor.orcid | Karlsson, Karl/0000-0002-5931-8422; Richert, Elena/0000-0003-0919-4879 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Sveinsdottir, Hildur Soley; Christensen, Christian; Porsteinsson, Haraldur; Karlsson, Karl Aegir] 3Z, Reykjavik, Iceland. | - |
local.description.affiliation | [Decker, Amanda; Cornell, Robert] Univ Iowa, Dept Anat & Cell Biol, Iowa City, IA USA. | - |
local.description.affiliation | [Lucena, Pablo Botella; Richert, Elena; Karlsson, Karl Aegir] Reykjavik Univ, Sch Sci & Engn, Reykjavik, Iceland. | - |
local.description.affiliation | [Richert, Elena] Carl von Ossietzky Univ Oldenburg, Dept Psychol, Oldenburg, Germany. | - |
local.description.affiliation | [Maier, Valerie Helene; Karlsson, Karl Aegir] Univ Iceland, Biomed Ctr, Reykjavik, Iceland. | - |
local.description.affiliation | [Lucena, Pablo Botella] Hasselt Univ, Biomed Res Inst, Dept Neurosci, Hasselt, Belgium. | - |
local.uhasselt.international | yes | - |
item.fullcitation | Sveinsdottir, Hildur Soley; Decker , Amanda; Christensen, Christian; BOTELLA LUCENA, Pablo; Porsteinsson, Haraldur; Richert, Elena; Maier, Valerie Helene; Cornell, Robert & Karlsson, Karl Aegir (2022) Motility phenotype in a zebrafish vmat2 mutant. In: PLoS One, 17 (1) (Art N° e0259753). | - |
item.validation | ecoom 2023 | - |
item.contributor | Sveinsdottir, Hildur Soley | - |
item.contributor | Decker , Amanda | - |
item.contributor | Christensen, Christian | - |
item.contributor | BOTELLA LUCENA, Pablo | - |
item.contributor | Porsteinsson, Haraldur | - |
item.contributor | Richert, Elena | - |
item.contributor | Maier, Valerie Helene | - |
item.contributor | Cornell, Robert | - |
item.contributor | Karlsson, Karl Aegir | - |
item.contributor | Neuhauss, Stephan C.F. | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
crisitem.journal.issn | 1932-6203 | - |
crisitem.journal.eissn | 1932-6203 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Motility phenotype in a zebrafish vmat2 mutant.pdf | Published version | 1.89 MB | Adobe PDF | View/Open |
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