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Title: | Novel Salmonella Phage, vB_Sen_STGO-35-1, Characterization and Evaluation in Chicken Meat | Authors: | Rivera, Dacil Moreno-Switt, Andrea, I Denes, Thomas G. Hudson, Lauren K. Peters, Tracey L. Samir, Reham Aziz, Ramy K. NOBEN, Jean-Paul WAGEMANS, Jan Duenas, Fernando |
Issue Date: | 2022 | Publisher: | MDPI | Source: | MICROORGANISMS, 10 (3) , p. 606 (Art N° 606) | Abstract: | Salmonellosis is one of the most frequently reported zoonotic foodborne diseases worldwide, and poultry is the most important reservoir of Salmonella enterica serovar Enteritidis. The use of lytic bacteriophages (phages) to reduce foodborne pathogens has emerged as a promising biocontrol intervention for Salmonella spp. Here, we describe and evaluate the newly isolated Salmonella phage STGO-35-1, including: (i) genomic and phenotypic characterization, (ii) an analysis of the reduction of Salmonella in chicken meat, and (iii) genome plasticity testing. Phage STGO-35-1 represents an unclassified siphovirus, with a length of 47,483 bp, a G + C content of 46.5%, a headful strategy of packaging, and a virulent lifestyle. Phage STGO-35-1 reduced S. Enteritidis counts in chicken meat by 2.5 orders of magnitude at 4 degrees C. We identified two receptor-binding proteins with affinity to LPS, and their encoding genes showed plasticity during an exposure assay. Phenotypic, proteomic, and genomic characteristics of STGO-35-1, as well as the Salmonella reduction in chicken meat, support the potential use of STGO-35-1 as a targeted biocontrol agent against S. Enteritidis in chicken meat. Additionally, computational analysis and a short exposure time assay allowed us to predict the plasticity of genes encoding putative receptor-binding proteins. | Notes: | Rivera, D (corresponding author), Univ Andres Bello, Fac Ciencias Vida, Escuela Med Vet, Santiago 8320000, Chile.; Rivera, D (corresponding author), Millennium Initiat Collaborat Res Bacterial Resis, Santiago 7550000, Chile. dacil.rivera@unab.cl; andrea.moreno@uc.cl; tdenes@utk.edu; lkhudson@utk.edu; tpeter21@vols.utk.edu; reham.samer@pharma.cu.edu.eg; ramy.aziz@gmail.com; jeanpaul.noben@uhasselt.be; jeroen.wagemans@kuleuven.be; ferdu26@gmail.com |
Keywords: | Salmonella Enteritidis; Salmonella-phage in food; Siphoviridae;;siphoviral morphotype; receptor-binding proteins | Document URI: | http://hdl.handle.net/1942/37239 | e-ISSN: | 2076-2607 | DOI: | 10.3390/microorganisms10030606 | ISI #: | WOS:000778265200001 | Rights: | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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microorganisms-10-00606-v4.pdf | Published version | 2.5 MB | Adobe PDF | View/Open |
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