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http://hdl.handle.net/1942/37398
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DC Field | Value | Language |
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dc.contributor.author | Mostafazadeh, Mostafa | - |
dc.contributor.author | KAHROBA, Houman | - |
dc.contributor.author | Haiaty, Sanya | - |
dc.contributor.author | TazeKand, Abbas P. | - |
dc.contributor.author | Samadi, Nasser | - |
dc.contributor.author | Rahbarghazi, Reza | - |
dc.contributor.author | Nouri, Mohammad | - |
dc.date.accessioned | 2022-06-02T06:31:32Z | - |
dc.date.available | 2022-06-02T06:31:32Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2022-05-13T16:33:04Z | - |
dc.identifier.citation | CELL BIOCHEMISTRY AND FUNCTION, | - |
dc.identifier.uri | http://hdl.handle.net/1942/37398 | - |
dc.description.abstract | Chemotherapy resistance is a serious pitfall in the treatment of colon cancers (CCs). Previous studies have found that exosomes (Exo) play a pivotal role in tumor drug resistance via the transfer of proteins and genetic materials to the acceptor cells. To date, the mechanisms orchestrating Exo-derived resistance in cancer cells have been the center of attention. Herein, we aimed to evaluate the role of exosomal nuclear factor erythroid 2-related factor 2 (Nrf2) on oxaliplatin (1-OHP) resistance in human colorectal cancer LS174T cells in vitro. To this end, exosomal-Nrf2-mediated 1-OHP resistance was examined using different assays. Exo was isolated from resistant LS174T cells (LS174T/R) and added to the culture medium of sensitive LS174T cells (LS174T/S). According to our data, LS174T/S cells successfully adsorbed PKH26-Exo driven from LS174T/R cells. Western blotting showed an increased Nrf2 level in Exo isolated from LS174T/R cells compared to LS174T/S cell-derived Exo (p < .05). The incubation of LS174T/S cells with LS174T/R-derived Exo increased half-maximal inhibitory concentration values in response to treatment with 1-OHP (p < .05). Besides this, the apoptotic changes were diminished in LS174T/S cells after incubation with LS174T/R-derived Exo. Of note, the exposure of LS174T/S cells to LS174T/R cell-derived Exo increased the expression of Nrf2 and P-glycoprotein (P-gp) compared to the nontreated LS174T/S cells (p < .05). In line with these changes, lower intracellular Rhodamin 123 content was detected in Exo-treated cells compared to the nontreated LS174T/S cells. Exo increased migration and clonogenic capacity of LS174T/S cells after incubation with Exo-derived from resistant cells. Of note, inhibition of Nrf2 with a specific blocker, brusatol, blunted these effects. Taken together, Exo-mediated transfer of Nrf2 is involved in the development of oxaliplatin resistance in CC cells by upregulating P-gp. | - |
dc.description.sponsorship | Tabriz University of Medical Sciences, Grant/Award Number: 97014455 This study was financially supported by the Drug Applied Research Center and Research ViceāChancellor of Tabriz University of Medical Sciences, Tabriz, Iran (PhD Thesis No. 59755) and Iran National Science Foundation (Grant No. 97014455). | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.rights | 2022 John Wiley & Sons Ltd. | | - |
dc.subject.other | chemoresistance | - |
dc.subject.other | exosomes | - |
dc.subject.other | human colorectal cancer LS174T cells | - |
dc.subject.other | Nrf2 | - |
dc.subject.other | oxaliplatin | - |
dc.title | In vitro exosomal transfer of Nrf2 led to the oxaliplatin resistance in human colorectal cancer LS174T cells | - |
dc.type | Journal Contribution | - |
local.format.pages | 12 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Rahbarghazi, R; Nouri, M (corresponding author), Tabriz Univ Med Sci, Stem Cell Res Ctr, Imam Reza St,Golgasht St, Tabriz 5166614756, Iran. | - |
dc.description.notes | Rezarahbardvm@gmail.com; Nourimd@yahoo.com | - |
local.publisher.place | 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.status | Early view | - |
dc.identifier.doi | 10.1002/cbf.3703 | - |
dc.identifier.pmid | 35474580 | - |
dc.identifier.isi | WOS:000787732100001 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Mostafazadeh, Mostafa; Samadi, Nasser; Nouri, Mohammad] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran. | - |
local.description.affiliation | [Mostafazadeh, Mostafa; TazeKand, Abbas P.; Samadi, Nasser; Nouri, Mohammad] Tabriz Univ Med Sci, Dept Biochem & Clin Labs, Tabriz, Iran. | - |
local.description.affiliation | [Kahroba, Houman] Maastricht Univ, GROW Sch Oncol & Dev Biol, Dept Toxicogen, Maastricht, Netherlands. | - |
local.description.affiliation | [Kahroba, Houman] Hasselt Univ, Ctr Environm Sci, Hasselt, Belgium. | - |
local.description.affiliation | [Haiaty, Sanya; Rahbarghazi, Reza] Tabriz Univ Med Sci, Res Ctr Infect Dis & Trop Med, Tabriz, Iran. | - |
local.description.affiliation | [Rahbarghazi, Reza] Tabriz Univ Med Sci, Stem Cell Res Ctr, Imam Reza St,Golgasht St, Tabriz 5166614756, Iran. | - |
local.description.affiliation | [Rahbarghazi, Reza] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Appl Cell Sci, Tabriz, Iran. | - |
local.uhasselt.international | yes | - |
item.fulltext | With Fulltext | - |
item.contributor | Mostafazadeh, Mostafa | - |
item.contributor | KAHROBA, Houman | - |
item.contributor | Haiaty, Sanya | - |
item.contributor | TazeKand, Abbas P. | - |
item.contributor | Samadi, Nasser | - |
item.contributor | Rahbarghazi, Reza | - |
item.contributor | Nouri, Mohammad | - |
item.accessRights | Open Access | - |
item.fullcitation | Mostafazadeh, Mostafa; KAHROBA, Houman; Haiaty, Sanya; TazeKand, Abbas P.; Samadi, Nasser; Rahbarghazi, Reza & Nouri, Mohammad (2022) In vitro exosomal transfer of Nrf2 led to the oxaliplatin resistance in human colorectal cancer LS174T cells. In: CELL BIOCHEMISTRY AND FUNCTION,. | - |
item.validation | ecoom 2023 | - |
crisitem.journal.issn | 0263-6484 | - |
crisitem.journal.eissn | 1099-0844 | - |
Appears in Collections: | Research publications |
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In vitro exosomal transfer of Nrf2 led to the oxaliplatin resistance in human colorectal cancer LS174T cells.pdf Restricted Access | Published version | 3.55 MB | Adobe PDF | View/Open Request a copy |
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