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Title: | The materialization of an impedimetric biosensor to detect papillomavirus DNA based on indium oxide nanowires | Authors: | SHOJAEI KOHNEHSHAHRI, Reza Emame, Sourena Ramezani Afrouz, Mohammad Mahdi Shariati, Mohsen |
Issue Date: | 2022 | Publisher: | SPRINGER HEIDELBERG | Source: | APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 128 (6) (Art N° 529) | Abstract: | In this study, the impedimetric biosensor of human papillomavirus DNA (HPV DNA) in label-free approach based on indium oxide nanowires (In2O3 NWs) was fabricated. The fabrication of In2O3 NWs was successfully conducted using the thermal evaporation method and catalyst-free approach. The grown NWs had a diameter of about 70-90 nm and a length of several microns. The fabricated electrode was able to show high selectivity by changing the relative charge transfer resistance of 80, 60 and 50% for complementary, mismatch and non-complementary sequences, respectively. The electrode biosensor was stable for up to 6 weeks under the effect of electric field and showed 93% of its initial response sensitivity and detected DNA hybridization at very low concentrations in a linear response range from 0.1 pM to 0.1 mu M. The In2O3 NWs showed a detection of limit (LOD) 20 fM. The behavior of negatively charged DNA oligonucleotides in a nanostructured array under the influence of electric field brought about NWs in the preferred direction with an excellent analytical response. Compared to current biosensors, the designed and fabricated biosensor, in a suitable sensing mechanism, was able to optimize response sensitivity and stability and also could improve the reproducibility conditions. | Notes: | Shariati, M (corresponding author), Sahand Univ Technol, Fac Sci, Dept Phys, Tabriz 5331817634, Iran. Shariatimohsen59@gmail.com |
Keywords: | Biosensor;Papillomavirus;In2O3 nanowires;Impedance spectroscopy;Label-free mechanism | Document URI: | http://hdl.handle.net/1942/37548 | ISSN: | 0947-8396 | e-ISSN: | 1432-0630 | DOI: | 10.1007/s00339-022-05674-y | ISI #: | WOS:000800565200001 | Rights: | There is no funding in this work | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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auteursversie.pdf | Peer-reviewed author version | 1.76 MB | Adobe PDF | View/Open |
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