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http://hdl.handle.net/1942/37667
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DC Field | Value | Language |
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dc.contributor.author | Erol, Gizem | - |
dc.contributor.author | Schmidt, Patricia Perez | - |
dc.contributor.author | PANCARO, Alessia | - |
dc.contributor.author | Diaz, Javier M. Melo | - |
dc.contributor.author | Barrientos, Africa G. | - |
dc.contributor.author | Porter, John | - |
dc.contributor.author | Polito, Laura | - |
dc.contributor.author | Szymonik, Michal | - |
dc.contributor.author | Nelissen, Inge | - |
dc.contributor.author | Spencer, Daniel I. R. | - |
dc.contributor.author | Piotti, Arianna | - |
dc.contributor.author | Beeg, Marten | - |
dc.contributor.author | De Simoni, Maria-Grazia | - |
dc.contributor.author | Fumagalli, Stefano | - |
dc.contributor.author | Gobbi, Marco | - |
dc.date.accessioned | 2022-07-07T09:47:46Z | - |
dc.date.available | 2022-07-07T09:47:46Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2022-07-07T09:23:25Z | - |
dc.identifier.citation | Sensors and actuators. B, Chemical (Print), 360 (Art N° 131661) | - |
dc.identifier.uri | http://hdl.handle.net/1942/37667 | - |
dc.description.abstract | Circulating mannose-binding lectin (MBL) plays an important role in the progression of tissue damage caused by ischemic events, an account of its high-affinity, multivalent binding to carbohydrate arrays exposed on damaged endothelium (damage-associated molecular patterns, DAMPS). MBL inhibitors have therefore been proposed as a novel protective therapeutic strategy to prevent secondary injury. We developed a new, convenient, robust surface plasmon resonance (SPR) assay for in vitro screening of compounds interfering with the binding of native human MBL to an appropriate chip surface functionalized with a pattern of sugar moieties mimicking DAMPS. We also characterized the procedure to regenerate the chip surface after each experimental session, accomplished by sequential cleaning with piranha solution followed by UV exposure. The SPR assay detects the specific binding of human recombinant MBL and native MBL present in human serum, and can identify inhibitors of this binding. We observed inhibitory effects of mannose (IC50 5 mM), of a nine mannose residues carrying glycan (IC50 0.33 mg/mL, corresponding to ~ 175 mu M), and mainly mannose-coated gold nanoparticles (IC50 1.1 mu g /mL). These in vitro results serve as a basis for testing the protective properties of these molecules/nanoparticles later inthe more expensive and time-consuming studies in cells and animal models of MBL mediated-injuries. | - |
dc.description.sponsorship | This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 814236. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.rights | 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). The authors wish to thank Mrs. Judith Baggott for English stylistic editing. | - |
dc.subject.other | Mannose binding lectin | - |
dc.subject.other | Glycans | - |
dc.subject.other | Nanoparticles | - |
dc.subject.other | Surface plasmon resonance | - |
dc.title | New nanostructures inhibiting human mannose binding lectin identified by a novel surface plasmon resonance assay | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 360 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Gobbi, M (corresponding author), Ist Ric Farmacol Mario Negri IRCCS, Via Mario Negri 2, I-20156 Milan, Italy. | - |
dc.description.notes | marco.gobbi@marionegri.it | - |
local.publisher.place | PO BOX 564, 1001 LAUSANNE, SWITZERLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 131661 | - |
local.type.programme | H2020 | - |
local.relation.h2020 | 814236 | - |
dc.identifier.doi | 10.1016/j.snb.2022.131661 | - |
dc.identifier.isi | 000812315500006 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Erol, Gizem; Piotti, Arianna; Beeg, Marten; De Simoni, Maria-Grazia; Fumagalli, Stefano; Gobbi, Marco] Ist Ric Farmacol Mario Negri IRCCS, Via Mario Negri 2, I-20156 Milan, Italy. | - |
local.description.affiliation | [Erol, Gizem] Univ Milano Bicocca, PhD Program Neurosci, Via Cadore 48, I-20900 Monza, MB, Italy. | - |
local.description.affiliation | [Schmidt, Patricia Perez; Polito, Laura] CNR, CNR SCITEC, Via G Fantoli 16-15, I-20138 Milan, Italy. | - |
local.description.affiliation | [Pancaro, Alessia; Szymonik, Michal; Nelissen, Inge] Flemish Inst Technol Res VITO Nv, Hlth Unit, Boeretang 200, B-2400 Mol, Belgium. | - |
local.description.affiliation | [Pancaro, Alessia] Hasselt Univ, Adv Opt Microscopy Ctr, Agoralaan Gebouw C, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Pancaro, Alessia] Hasselt Univ, Biomed Res Inst, Agoralaan Gebouw C, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Diaz, Javier M. Melo; Spencer, Daniel I. R.] Ludger Ltd, Culham Sci Ctr, Abingdon OX14 3EB, Oxon, England. | - |
local.description.affiliation | [Diaz, Javier M. Melo] Royal Coll Surgeons Ireland, Dept Chem, 123 St Stephens Green, Dublin D02 YN77 2, Ireland. | - |
local.description.affiliation | [Barrientos, Africa G.; Porter, John] Midatech Pharma PLC, Oddfellows House,19 Newport Rd, Cardiff CF24 0AA, Wales. | - |
local.description.affiliation | [Barrientos, Africa G.] I Med S Coop, Parque Tecnol Alava, Vitoria 01510, Alava, Spain. | - |
local.uhasselt.international | yes | - |
item.fullcitation | Erol, Gizem; Schmidt, Patricia Perez; PANCARO, Alessia; Diaz, Javier M. Melo; Barrientos, Africa G.; Porter, John; Polito, Laura; Szymonik, Michal; Nelissen, Inge; Spencer, Daniel I. R.; Piotti, Arianna; Beeg, Marten; De Simoni, Maria-Grazia; Fumagalli, Stefano & Gobbi, Marco (2022) New nanostructures inhibiting human mannose binding lectin identified by a novel surface plasmon resonance assay. In: Sensors and actuators. B, Chemical (Print), 360 (Art N° 131661). | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2023 | - |
item.contributor | Erol, Gizem | - |
item.contributor | Schmidt, Patricia Perez | - |
item.contributor | PANCARO, Alessia | - |
item.contributor | Diaz, Javier M. Melo | - |
item.contributor | Barrientos, Africa G. | - |
item.contributor | Porter, John | - |
item.contributor | Polito, Laura | - |
item.contributor | Szymonik, Michal | - |
item.contributor | Nelissen, Inge | - |
item.contributor | Spencer, Daniel I. R. | - |
item.contributor | Piotti, Arianna | - |
item.contributor | Beeg, Marten | - |
item.contributor | De Simoni, Maria-Grazia | - |
item.contributor | Fumagalli, Stefano | - |
item.contributor | Gobbi, Marco | - |
item.accessRights | Open Access | - |
crisitem.journal.eissn | 0925-4005 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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New nanostructures inhibiting human mannose binding lectin identified by a novel surface plasmon resonance assay.pdf | Published version | 3.56 MB | Adobe PDF | View/Open |
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