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http://hdl.handle.net/1942/37795
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DC Field | Value | Language |
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dc.contributor.author | Yongabi, Derick | - |
dc.contributor.author | KHORSHID, Mehran | - |
dc.contributor.author | LOSADA-PEREZ, Patricia | - |
dc.contributor.author | Sichani, Soroush Bakhshi | - |
dc.contributor.author | Jooken , Stijn | - |
dc.contributor.author | Stilman, Wouter | - |
dc.contributor.author | Thesseling, Florian | - |
dc.contributor.author | Martens, Tobie | - |
dc.contributor.author | Van Thillo , Toon | - |
dc.contributor.author | Verstrepen, Kevin | - |
dc.contributor.author | Dedecker , Peter | - |
dc.contributor.author | Vanden Berghe, Pieter | - |
dc.contributor.author | Lettinga, Minne Paul | - |
dc.contributor.author | Bartic, Carmen | - |
dc.contributor.author | Lieberzeit, Peter | - |
dc.contributor.author | Schoning, Michael J. | - |
dc.contributor.author | THOELEN, Ronald | - |
dc.contributor.author | Fransen, Marc | - |
dc.contributor.author | Wubbenhorst, Michael | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.date.accessioned | 2022-07-25T07:10:12Z | - |
dc.date.available | 2022-07-25T07:10:12Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2022-07-19T13:56:52Z | - |
dc.identifier.citation | Advanced science (Weinheim), (Art N° 2200459) | - |
dc.identifier.uri | http://hdl.handle.net/1942/37795 | - |
dc.description.abstract | Despite the importance of cell characterization and identification for diagnostic and therapeutic applications, developing fast and label-free methods without (bio)-chemical markers or surface-engineered receptors remains challenging. Here, we exploit the natural cellular response to mild thermal stimuli and propose a label- and receptor-free method for fast and facile cell characterization. Cell suspensions in a dedicated sensor are exposed to a temperature gradient, which stimulates synchronized and spontaneous cell-detachment with sharply defined time-patterns, a phenomenon unknown from literature. These patterns depend on metabolic activity (controlled through temperature, nutrients, and drugs) and provide a library of cell-type-specific indicators, allowing to distinguish several yeast strains as well as cancer cells. Under specific conditions, synchronized glycolytic-type oscillations are observed during detachment of mammalian and yeast-cell ensembles, providing additional cell-specific signatures. These findings suggest potential applications for cell viability analysis and for assessing the collective response of cancer cells to drugs. | - |
dc.description.sponsorship | This project was funded by the KU Leuven project C14/15/066 “Smart Cellular Scaffolds”, the Research Foundation Flanders FWO, project G.0791.16N “Utilizing interfacial impedance- and heat-transfer phenomena in advanced monitoring- and switching devices,” and the bilateral FWO Flanders—FWF Austria project “Simultaneous multiparametric readout for low-cost nanoparticle sensors”. P.V.B. acknowledges funding from Hercules AKUL/11/37 and FWO G.0929.15. T.M. acknowledges funding from FWO I000321N project. The authors thank Prof. Kasper Eersels (Maastricht University, The Netherlands), Prof. Jef Hooyberghs (VITO, Belgium), and Prof. Ward De Ceuninck (Hasselt University, Belgium) for stimulating discussions. The authors appreciate Dr. Peter Cornelis, Dr. Kuo Zhong, Dr. Gideon Wackers, Dr. Celien Lismont, Dr. Wouter Van Genechten, Drs. Christiana Adebayo, and Mr. Werner Neefs (all of KU Leuven, Belgium), as well as Dr. Heiko Iken (FH Aachen) for their technical assistance. | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.rights | 2022 The Authors. Advanced Science published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | - |
dc.subject.other | cancer therapy | - |
dc.subject.other | cell characterization | - |
dc.subject.other | cell detection | - |
dc.subject.other | glycolytic oscillations | - |
dc.subject.other | heat-transfer method | - |
dc.subject.other | metabolic activity | - |
dc.subject.other | spontaneous cell detachment | - |
dc.title | Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification | - |
dc.type | Journal Contribution | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Yongabi, D; Wagner, P (corresponding author), Katholieke Univ Leuven, Dept Phys & Astron, Lab Soft Matter & Biophys, Celestijnenlaan 200 D, B-3001 Leuven, Belgium. | - |
dc.description.notes | derick.yongabi@kuleuven.be; patrickhermann.wagner@kuleuven.be | - |
local.publisher.place | 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.status | Early view | - |
local.bibliographicCitation.artnr | 2200459 | - |
dc.identifier.doi | 10.1002/advs.202200459 | - |
dc.identifier.pmid | 35780480 | - |
dc.identifier.isi | 000819977500001 | - |
dc.contributor.orcid | Fransen, Marc/0000-0001-9284-1197; Wagner, Patrick/0000-0002-4028-3629; | - |
dc.contributor.orcid | Vanden Berghe, Pieter/0000-0002-0009-2094 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Yongabi, Derick; Khorshid, Mehran; Sichani, Soroush Bakhshi; Jooken, Stijn; Stilman, Wouter; Lettinga, Minne Paul; Bartic, Carmen; Wubbenhorst, Michael; Wagner, Patrick] Katholieke Univ Leuven, Dept Phys & Astron, Lab Soft Matter & Biophys, Celestijnenlaan 200 D, B-3001 Leuven, Belgium. | - |
local.description.affiliation | [Losada-Perez, Patricia] Univ Libre Bruxelles, Fac Sci Expt Soft Matter & Thermal Phys EST, Blvd Triomphe ACC 2, B-1050 Brussels, Belgium. | - |
local.description.affiliation | [Thesseling, Florian; Verstrepen, Kevin] Katholieke Univ Leuven, Dept Microbial & Mol Syst, VIB Ctr Microbiol, Lab Syst Biol, Gaston Geenslaan 1, B-3001 Heverlee, Belgium. | - |
local.description.affiliation | [Martens, Tobie; Vanden Berghe, Pieter] Katholieke Univ Leuven, Dept Chron Dis Metab & Ageing, Lab Enter Neurosci LENS, B-3000 Leuven, Belgium. | - |
local.description.affiliation | [Van Thillo, Toon; Dedecker, Peter] Katholieke Univ Leuven, Biochem Mol & Struct Biol, Celestijnenlaan 200 G, B-3001 Leuven, Belgium. | - |
local.description.affiliation | [Lettinga, Minne Paul] Res Ctr Julich GmbH, Biomacromol Syst & Proc IBI 4, Leo Brandt Str, D-52425 Julich, Germany. | - |
local.description.affiliation | [Lieberzeit, Peter] Univ Vienna, Fac Chem, Dept Phys Chem, Str 38, A-1090 Vienna, Austria. | - |
local.description.affiliation | [Schoning, Michael J.] Aachen Univ Appl Sci, Inst Nano & Biotechnol INB, Heinrich Mussmann Str 1, D-52428 Julich, Germany. | - |
local.description.affiliation | [Thoelen, Ronald] Hasselt Univ, Inst Mat Res, Wetenschapspk 1, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Fransen, Marc] Katholieke Univ Leuven, Dept Cellular & Mol Med, Lab Peroxisome Biol & Intracellular Commun, Herestr 49, B-3000 Leuven, Belgium. | - |
local.uhasselt.international | yes | - |
item.fullcitation | Yongabi, Derick; KHORSHID, Mehran; LOSADA-PEREZ, Patricia; Sichani, Soroush Bakhshi; Jooken , Stijn; Stilman, Wouter; Thesseling, Florian; Martens, Tobie; Van Thillo , Toon; Verstrepen, Kevin; Dedecker , Peter; Vanden Berghe, Pieter; Lettinga, Minne Paul; Bartic, Carmen; Lieberzeit, Peter; Schoning, Michael J.; THOELEN, Ronald; Fransen, Marc; Wubbenhorst, Michael & WAGNER, Patrick (2022) Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells: A New Tool for Cell Characterization and Identification. In: Advanced science (Weinheim), (Art N° 2200459). | - |
item.fulltext | With Fulltext | - |
item.validation | ecoom 2023 | - |
item.contributor | Yongabi, Derick | - |
item.contributor | KHORSHID, Mehran | - |
item.contributor | LOSADA-PEREZ, Patricia | - |
item.contributor | Sichani, Soroush Bakhshi | - |
item.contributor | Jooken , Stijn | - |
item.contributor | Stilman, Wouter | - |
item.contributor | Thesseling, Florian | - |
item.contributor | Martens, Tobie | - |
item.contributor | Van Thillo , Toon | - |
item.contributor | Verstrepen, Kevin | - |
item.contributor | Dedecker , Peter | - |
item.contributor | Vanden Berghe, Pieter | - |
item.contributor | Lettinga, Minne Paul | - |
item.contributor | Bartic, Carmen | - |
item.contributor | Lieberzeit, Peter | - |
item.contributor | Schoning, Michael J. | - |
item.contributor | THOELEN, Ronald | - |
item.contributor | Fransen, Marc | - |
item.contributor | Wubbenhorst, Michael | - |
item.contributor | WAGNER, Patrick | - |
item.accessRights | Open Access | - |
crisitem.journal.eissn | 2198-3844 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Synchronized, Spontaneous, and Oscillatory Detachment of Eukaryotic Cells_ A New Tool for Cell Characterization and Identification.pdf | Published version | 5.45 MB | Adobe PDF | View/Open |
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