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http://hdl.handle.net/1942/37861
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DC Field | Value | Language |
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dc.contributor.author | Laporte , Emma | - |
dc.contributor.author | HERMANS, Florian | - |
dc.contributor.author | De Vriendt, Silke | - |
dc.contributor.author | Vennekens, Annelies | - |
dc.contributor.author | Lambrechts, Diether | - |
dc.contributor.author | Nys, Charlotte | - |
dc.contributor.author | Cox , Benoit | - |
dc.contributor.author | Vankelecom, Hugo | - |
dc.date.accessioned | 2022-08-17T09:10:36Z | - |
dc.date.available | 2022-08-17T09:10:36Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2022-08-17T08:51:47Z | - |
dc.identifier.citation | eLife (Cambridge), 11 (Art N° e75742) | - |
dc.identifier.uri | http://hdl.handle.net/1942/37861 | - |
dc.description.abstract | The pituitary represents the endocrine master regulator. In mouse, the gland undergoes active maturation immediately after birth. Here, we in detail portrayed the stem cell compartment of neonatal pituitary. Single-cell RNA-sequencing pictured an active gland, revealing proliferative stem as well as hormonal (progenitor) cell populations. The stem cell pool displayed a hybrid epithelial/mesenchymal phenotype, characteristic of development-involved tissue stem cells. Organoid culturing recapitulated the stem cells' phenotype, interestingly also reproducing their paracrine activity. The pituitary stem cell-activating interleukin-6 advanced organoid growth, although the neonatal stem cell compartment was not visibly affected in Il6(-/-) mice, likely due to cytokine family redundancy. Further transcriptomic analysis exposed a pronounced WNT pathway in the neonatal gland, shown to be involved in stem cell activation and to overlap with the (fetal) human pituitary transcriptome. Following local damage, the neonatal gland efficiently regenerates, despite absence of additional stem cell proliferation, or upregulated IL-6 or WNT expression, all in line with the already high stem cell activation status, thereby exposing striking differences with adult pituitary. Together, our study decodes the stem cell compartment of neonatal pituitary, exposing an activated state in the maturing gland. Understanding stem cell activation is key to potential pituitary regenerative prospects. | - |
dc.description.sponsorship | We thank Y Van Goethem and V Vanslembrouck for valuable technical help. We are also grateful to the Imaging Core (VIB, KU Leuven) and the CIC (KU Leuven) for use of microscopes and the Center for Brain & Disease Research (CBD) Histology unit (VIB, KU Leuven) for use of histology equipment. We are indebted to Dr Pura Muñoz-Cánoves (Universitat Pompeu Fabra, Barcelona, Spain) for generously providing the Il6−/− mice. We thank Thomas Van Brussel, Rogier Schepers, and Bram Boeckx (D L’s group, KU Leuven) for technical and bioinformatical support in scRNA-seq experiments. The computational resources used for scRNA-seq analysis were provided by the ‘Vlaams Supercomputer Centrum’, managed by the Fund for Scientific Research (FWO)—Flanders. We also thank the FACS core (KU Leuven) for training and use of the MESO QuickPlex SQ 120 reader. Finally, we recognize the Laboratory of Virology and Chemotherapy (Rega Institute; Dr D Daelemans) for experimental help and use of the IncuCyte S3. Funding: This work was supported by grants from the KU Leuven Research Fund and from the Fund for Scientific Research (FWO)—Flanders. E L (11A3320N), A V (1141717 N), C N (1S14218N), and B C (11W9215N) are supported by a PhD Fellowship from the FWO/FWO-SB. Use of the Zeiss LSM 780 – SP Mai Tai HP DS is supported by Hercules AKUL/11/37 and FWO G.0929.15 funding to Dr P Vanden Berghe (CIC, KU Leuven). The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication. | - |
dc.language.iso | en | - |
dc.publisher | eLIFE SCIENCES PUBL LTD | - |
dc.rights | Copyright Laporte et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. | - |
dc.subject.other | pituitary | - |
dc.subject.other | stem cells | - |
dc.subject.other | single-cell RNA-sequencing | - |
dc.subject.other | WNT signaling | - |
dc.subject.other | organoids | - |
dc.subject.other | tissue maturation | - |
dc.subject.other | Mouse | - |
dc.title | Decoding the activated stem cell phenotype of the neonatally maturing pituitary | - |
dc.type | Journal Contribution | - |
dc.identifier.volume | 11 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Vankelecom, H (corresponding author), Katholieke Univ Leuven, Lab Tissue Plast Hlth & Dis, Dept Dev & Regenerat, Cluster Stem Cell & Dev Biol, Leuven, Belgium. | - |
dc.description.notes | hugo.vankelecom@kuleuven.be | - |
local.publisher.place | SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | e75742 | - |
dc.identifier.doi | 10.7554/eLife.75742 | - |
dc.identifier.pmid | 35699412 | - |
dc.identifier.isi | 000834159000001 | - |
dc.contributor.orcid | Laporte, Emma/0000-0003-0799-3116; Cox, Benoit/0000-0002-3139-268X; | - |
dc.contributor.orcid | Lambrechts, Diether/0000-0002-3429-302X; De Vriendt, | - |
dc.contributor.orcid | Silke/0000-0002-4022-2869; Vankelecom, Hugo/0000-0002-2251-7284; | - |
dc.contributor.orcid | Hermans, Florian/0000-0002-2321-3995 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Laporte, Emma; Hermans, Florian; De Vriendt, Silke; Vennekens, Annelies; Nys, Charlotte; Cox, Benoit; Vankelecom, Hugo] Katholieke Univ Leuven, Lab Tissue Plast Hlth & Dis, Dept Dev & Regenerat, Cluster Stem Cell & Dev Biol, Leuven, Belgium. | - |
local.description.affiliation | [Hermans, Florian] Hasselt Univ, Biomed Res Inst, Lab Morphol, Diepenbeek, Belgium. | - |
local.description.affiliation | [Lambrechts, Diether] VIB, Ctr Canc Biol, Leuven, Belgium. | - |
local.description.affiliation | [Lambrechts, Diether] Katholieke Univ Leuven, Dept Human Genet, Lab Translat Genet, Leuven, Belgium. | - |
local.uhasselt.international | no | - |
item.validation | ecoom 2023 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.fullcitation | Laporte , Emma; HERMANS, Florian; De Vriendt, Silke; Vennekens, Annelies; Lambrechts, Diether; Nys, Charlotte; Cox , Benoit & Vankelecom, Hugo (2022) Decoding the activated stem cell phenotype of the neonatally maturing pituitary. In: eLife (Cambridge), 11 (Art N° e75742). | - |
item.contributor | Laporte , Emma | - |
item.contributor | HERMANS, Florian | - |
item.contributor | De Vriendt, Silke | - |
item.contributor | Vennekens, Annelies | - |
item.contributor | Lambrechts, Diether | - |
item.contributor | Nys, Charlotte | - |
item.contributor | Cox , Benoit | - |
item.contributor | Vankelecom, Hugo | - |
crisitem.journal.issn | 2050-084X | - |
crisitem.journal.eissn | 2050-084X | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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elife-75742-v2.pdf | Published version | 8.22 MB | Adobe PDF | View/Open |
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