Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/37951
Title: | Acute inhibition of transient receptor potential vanilloid-type 4 cation channel halts cytoskeletal dynamism in microglia | Authors: | BEEKEN, Jolien MERTENS, Melanie Stas, Nathan KESSELS, Sofie AERTS, Liese JANSSEN, Bieke Mussen, Femke Pinto, Silvia Vennekens , Rudi RIGO, Jean-Michel Nguyen, Laurent BRONE, Bert AGUIAR ALPIZAR, Yeranddy |
Issue Date: | 2022 | Publisher: | WILEY | Source: | GLIA (New York, N.Y. : Print), 70 (11) , p. 2157-2168 | Abstract: | Microglia, the resident macrophages of the central nervous system, are highly motile cells that support brain development, provision neuronal signaling, and protect brain cells against damage. Proper microglial functioning requires constant cell movement and morphological changes. Interestingly, the transient receptor potential vanilloid 4 (TRPV4) channel, a calcium-permeable channel, is involved in hypoosmotic morphological changes of retinal microglia and regulates temperature-dependent movement of microglial cells both in vitro and in vivo. Despite the broad functions of TRPV4 and the recent findings stating a role for TRPV4 in microglial movement, little is known about how TRPV4 modulates cytoskeletal remodeling to promote changes of microglial motility. Here we show that acute inhibition of TRPV4, but not its constitutive absence in the Trpv4 KO cells, affects the morphology and motility of microglia in vitro. Using high-end confocal imaging techniques, we show a decrease in actin-rich filopodia and tubulin dynamics upon acute inhibition of TRPV4 in vitro. Furthermore, using acute brain slices we demonstrate that Trpv4 knockout microglia display lower ramification complexity, slower process extension speed and consequently smaller surveyed area. We conclude that TRPV4 inhibition triggers a shift in cytoskeleton remodeling of microglia influencing their migration and morphology. | Notes: | Brone, B; Alpizar, YA (corresponding author), UHasselt, BIOMED, Diepenbeek, Belgium. bert.brone@uhasselt.be; yeranddy.aguiaralpizar@uhasselt.be |
Keywords: | cytoskeleton;microglia;morphology;process motility;persistent random walk;TRPV4 | Document URI: | http://hdl.handle.net/1942/37951 | ISSN: | 0894-1491 | e-ISSN: | 1098-1136 | DOI: | 10.1002/glia.24243 | ISI #: | 000822391400001 | Rights: | 2022 Wiley Periodicals LLC | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Acute inhibition of transient receptor potential vanilloid‐type 4 cation channel halts cytoskeletal dynamism in microglia.pdf Restricted Access | Published version | 3.62 MB | Adobe PDF | View/Open Request a copy |
Beeken_2022_GLIAmanuscript author version.pdf | Peer-reviewed author version | 4.48 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.