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Title: | Paediatric pre-B acute lymphoblastic leukaemia-derived exosomes regulate immune function in human T cells | Authors: | Gholipour, Elham KAHROBA, Houman Soltani, Nasim Samadi, Parisa Sarvarian, Parisa Vakili-Samiani, Sajjad Hosein Pour Feizi, Abbas Ali Soltani-Zangbar, Mohammad Sadegh Baghersalimi, Adel Darbandi, Bahram Movassaghpour, Aliakbar Talebi, Mehdi Motavalli, Roza Mehdizadeh, Amir Kazemi, Abdolhassan Yousefi, Mehdi |
Issue Date: | 2022 | Publisher: | WILEY | Source: | Journal of Cellular and Molecular Medicine (Print), 26 (16) , p. 4566 -4576 | Abstract: | Exosomes derived from solid tumour cells are involved in immune suppression, angiogenesis and metastasis; however, the role of leukaemia-derived exosomes has less been investigated. Hence, changes in immune response-related genes and human T cells apoptosis co-incubated with exosomes isolated from patients' pre-B cell acute lymphoblastic leukaemia were evaluated in this in vitro study. Vein blood sample was obtained from each newly diagnosed acute lymphoblastic leukaemia (ALL) patient prior any therapy. ALL serum exosomes were isolated by ultrafiltration and characterized using Western blotting and transmission electron microscopy. Exosomes were then co-incubated with T lymphocytes and the gene expressions, as well as functions of human T cells were quantified by qRT-PCR. Apoptosis and caspase-3 and caspase-9 protein expression were also evaluated by flowcytometry and Western blotting analysis, respectively. Exosomes isolated from ALL patients affected T lymphocytes and elevated the apoptosis. Moreover, these exosomes altered the T cells profile into regulatory type by increasing the expression of FOXP3 and Tregs-related cytokines, including TGF-B and IL-10. The expression level of Th17-related transcription factors (RoR gamma t) and interleukins (IL-17 and IL-23) decreased after this treatment. According to our findings, exosomes derived from ALL patients' sera carry immunosuppressive molecules, indicating the possible effect of exosomes as liquid biomarkers for cancer staging. | Notes: | Yousefi, M (corresponding author), Tabriz Univ Med Sci, Fac Med, Dept Immunol, Tabriz, Iran. yousefime@tbzmed.ac.ir |
Keywords: | CD8+T cells;immunosuppression;mRNA;regulatory T cell;tumour-derived exosome | Document URI: | http://hdl.handle.net/1942/37979 | ISSN: | 1582-1838 | e-ISSN: | 1582-4934 | DOI: | 10.1111/jcmm.17482 | ISI #: | 000823513400001 | Rights: | 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2023 |
Appears in Collections: | Research publications |
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Paediatric pre‐B acute lymphoblastic leukaemia‐derived exosomes regulate immune function in human T cells.pdf | Published version | 3.72 MB | Adobe PDF | View/Open |
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