Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/38014
Title: | Immune phenotyping SCI patients versus healthy controls | Data Creator - person: | FRAUSSEN, Judith BECKERS, Lien van Laake-Geelen, Charlotte Depreitere, Bart Deckers, Jens Cornips, Erwin peuskens, diederik SOMERS, Veerle |
Data Creator - organization: | Hasselt University Maastricht University University Hospitals Leuven Algemeen Ziekenhuis Turnhout Ziekenhuis Oost-Limburg Adelante Centre of Expertise in Rehabilitation and Audiology |
Data Curator - person: | FRAUSSEN, Judith | Data Curator - organization: | Hasselt University | Rights Holder - person: | FRAUSSEN, Judith | Rights Holder - organization: | Hasselt University | Publisher: | Open Data Commons for Spinal Cord Injury (ODC-SCI) | Issue Date: | 2022 | Abstract: | STUDY PURPOSE: Following a spinal cord injury (SCI), an inflammatory immune reaction is triggered which results in advanced secondary tissue damage. The systemic post-SCI immune response is poorly understood. This study aimed to extensively analyse the circulating immune cell composition in traumatic SCI patients in relation to clinical parameters. DATA COLLECTED: High-dimensional flow cytometry was performed on peripheral blood mononuclear cells of 18 traumatic SCI patients and 18 healthy controls to determine immune cell subsets. SCI blood samples were collected at multiple time points in the (sub)acute (0 days to 3 weeks post-SCI, (s)aSCI) and chronic (6 to >18 weeks post-SCI, cSCI) disease phase. Not all time points were available for every included SCI patient due to organizational issues (late inclusion) or withdrawal of subjects from the study. Twelve SCI patients presented with a cervical injury, 5 with a thoracic injury and 1 with a lumbar injury. We first analyzed the major immune cell subsets in PBMC of HC, (s)aSCI patients and cSCI patients. These included CD14+ monocytes, natural killer (NK) cells, which are classically divided into cytotoxic CD56loCD16+ and highly cytokine producing CD56hiCD16+/- subsets, CD19+ B cells and CD3+ T cells, divided into CD4+ helper and CD8+ cytotoxic subsets. Next, the following subsets were studied in both CD4+ and CD8+ T cells: naive (Tnaive, CD45RA+CD45RO-), memory (Tm, CD45RA-CD45RO+), effector memory (Tem, CD45RA-CCR7-), central memory (Tcm, CD45RA-CCR7+), effector memory re-expressing CD45RA (Temra, CD45RA+CCR7-) and regulatory (Treg, CD25+CD127low). CD19+ B cells were studied in more detail by analysis of CD24hiCD38hi transitional, IgD+CD27- naive, IgD+CD27+ non class-switched memory (NCSM), IgD-CD27+ class-switched memory (CSM), IgM+IgD-CD27+ IgM only and IgD-CD27- double negative (DN) subsets. IgM, IgG and IgA were included to further define memory responses. DATA USAGE NOTES: | Research Discipline: | Medical and health sciences > Clinical sciences > Immunology > Immunology not elsewhere classified (03021499) Medical and health sciences > Clinical sciences > Neurosciences > Neurosciences not elsewhere classified (03022399) |
Keywords: | immune profiling;human;B cell;flow cytometry | DOI: | 10.34945/F5588X | Link to publication/dataset: | https://odc-sci.org/data/774 | Source: | Open Data Commons for Spinal Cord Injury (ODC-SCI). 10.34945/F5588X https://odc-sci.org/data/774 | Publications related to the dataset: | 10.3389/fimmu.2022.873315 | License: | Creative Commons Attribution 4.0 International (CC-BY-4.0) | Access Rights: | Open Access | Category: | DS | Type: | Dataset |
Appears in Collections: | Datasets |
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.