Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/38030
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dc.contributor.authorGodschalk, Roger W. L.-
dc.contributor.authorJanssen, Margit C. M.-
dc.contributor.authorVANHEES, Kimberly-
dc.contributor.authorvan Doorn-Khosrovani, Sahar Barjesteh van Waalwijk-
dc.contributor.authorVAN SCHOOTEN, Frederik-Jan-
dc.date.accessioned2022-09-08T14:32:57Z-
dc.date.available2022-09-08T14:32:57Z-
dc.date.issued2022-
dc.date.submitted2022-08-17T11:03:31Z-
dc.identifier.citationFrontiers in nutrition, 9 (Art N° 904368)-
dc.identifier.urihttp://hdl.handle.net/1942/38030-
dc.description.abstractBackgroundGenistein is a dietary supplement with phyto-estrogenic properties. Therefore, high intake of genistein during pregnancy may have adverse effects on the genetic integrity of testes and germ cells of male offspring. In this study, we examined whether maternal exposure to genistein during pregnancy induced oxidative DNA damage in the male germline at adolescence. MethodsAtm-Delta SRI mice have lower glucose-6-phosphate dehydrogenase (G6PDH) activity, which is important for maintaining levels of reduced glutathione and therefore these mice have an increased susceptibility to oxidative stress. Parental heterozygous Atm-Delta SRI mice received a genistein-rich or control diet, after which they were mated to obtain offspring. During pregnancy, mothers remained on the respective diets and after delivery all animals received control diets. Redox status and oxidative DNA damage were assessed in testes and sperm of 12 weeks old male offspring. Gene expression of Cyp1b1, Comt, and Nqo1 was assessed in testes, and DNA methylation as possible mechanism for transmission of effects to later life. ResultsIntake of genistein during pregnancy increased oxidative DNA damage in testes of offspring, especially in heterozygous Atm-Delta SRI mice. These increased DNA damage levels coincided with decreased expression of Comt and Nqo1. Heterozygous Atm-Delta SRI mice had higher levels of DNA strand breaks in sperm compared to wild type littermates, and DNA damage was further enhanced by a genistein-rich maternal diet. G6PDH activity was higher in mice with high maternal intake of genistein compared to control diets, suggesting compensation against oxidative stress. A positive correlation was observed between the levels of DNA methylation and oxidative DNA damage in testes. ConclusionThese data indicate that prenatal exposure to genistein altered gene expression and increased DNA damage in testes and sperm of adolescent male offspring. These effects of genistein on DNA damage in later life coincided with alterations in DNA methylation.-
dc.description.sponsorshipThis work was partly supported by grant number 06A031 from the American Institute for Cancer Research.-
dc.language.isoen-
dc.publisherFRONTIERS MEDIA SA-
dc.rights© 2022 Godschalk, Janssen, Vanhees, Doorn-Khosrovani and Schooten. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.-
dc.subject.othergenistein-
dc.subject.otherestrogen metabolism-
dc.subject.othertestes-
dc.subject.otherantioxidant system-
dc.subject.otherDNA damage-
dc.titleMaternal exposure to genistein during pregnancy and oxidative DNA damage in testes of male mouse offspring-
dc.typeJournal Contribution-
dc.identifier.volume9-
local.bibliographicCitation.jcatA1-
dc.description.notesGodschalk, RWL (corresponding author), Maastricht Univ, Sch Nutr & Translat Res Metab NUTRIM, Dept Pharmacol & Toxicol, Maastricht, Netherlands.-
dc.description.notesr.godschalk@maastrichtuniversity.nl-
local.publisher.placeAVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr904368-
dc.identifier.doi10.3389/fnut.2022.904368-
dc.identifier.pmid35923192-
dc.identifier.isi000835526800001-
local.provider.typewosris-
local.description.affiliation[Godschalk, Roger W. L.; Janssen, Margit C. M.; Vanhees, Kimberly; van Doorn-Khosrovani, Sahar Barjesteh van Waalwijk; van Schooten, Frederik-Jan] Maastricht Univ, Sch Nutr & Translat Res Metab NUTRIM, Dept Pharmacol & Toxicol, Maastricht, Netherlands.-
local.description.affiliation[Vanhees, Kimberly] Univ Biobank Limburg, Jessa Ziekenhuis, Hasselt, Belgium.-
local.description.affiliation[van Doorn-Khosrovani, Sahar Barjesteh van Waalwijk] CZ Hlth Insurances, Dept Med Advisory & Innovat, Tilburg, Netherlands.-
local.uhasselt.internationalyes-
item.validationecoom 2023-
item.fulltextWith Fulltext-
item.fullcitationGodschalk, Roger W. L.; Janssen, Margit C. M.; VANHEES, Kimberly; van Doorn-Khosrovani, Sahar Barjesteh van Waalwijk & VAN SCHOOTEN, Frederik-Jan (2022) Maternal exposure to genistein during pregnancy and oxidative DNA damage in testes of male mouse offspring. In: Frontiers in nutrition, 9 (Art N° 904368).-
item.accessRightsOpen Access-
item.contributorGodschalk, Roger W. L.-
item.contributorJanssen, Margit C. M.-
item.contributorVANHEES, Kimberly-
item.contributorvan Doorn-Khosrovani, Sahar Barjesteh van Waalwijk-
item.contributorVAN SCHOOTEN, Frederik-Jan-
crisitem.journal.issn2296-861X-
crisitem.journal.eissn2296-861X-
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