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http://hdl.handle.net/1942/3836
Title: | Construction and expression of antibody tumor-necrosis-factor fusion proteins | Authors: | HOOGENBOOM, HR VOLCKAERT, G RAUS, Jef |
Issue Date: | 1991 | Publisher: | PERGAMON-ELSEVIER SCIENCE LTD | Source: | MOLECULAR IMMUNOLOGY, 28(9). p. 1027-1037 | Abstract: | The construction, expression and secretion of two genetically engineered antibody-cytokine hybrid fusion proteins is described. To target tumor necrosis factor (TNF) to tumor cells, recombinant antibody techniques were used to generate F(ab')2-like antibody-TNF fusion proteins. At the gene level, an antitransferrin receptor antibody heavy chain gene was linked to a synthetic gene coding for human TNF. The chimeric heavy chain-TNF genes were introduced into a light chain secreting transfectoma cell line, which was producing the light chain of the same antibody. Cell lines were isolated which secreted antibody-TNF fusion proteins of expected size and composition. Culture supernatant of these cell lines contained TNF cytotoxic activity towards murine L929 cells and human MCF-7 cells, indicating that TNF is still active in the fusion protein constructs. These results illustrate the feasibility of the antibody engineering technology to create and produce chimeric mouse-human immunotoxin-like molecules. Furthermore, they demonstrate the ability of mammalian (myeloma) cells to express and secrete antibody-cytokine hybrid molecules with potential use in anticancer therapy. | Notes: | LIMBURGS UNIV CENTRUM,DR L WILLEMS INST,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM. LIMBURGS UNIV CENTRUM,DEPT WNIF,B-3590 DIEPENBEEK,BELGIUM. CATHOLIC UNIV LEUVEN,GENTECHNOL LAB,B-3030 HEVERLEE,BELGIUM. | Document URI: | http://hdl.handle.net/1942/3836 | DOI: | 10.1016/0161-5890(91)90189-Q | ISI #: | A1991GJ82700013 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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