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http://hdl.handle.net/1942/3854
Title: | Regulation of myelin basic protein-specific helper T-cells in multiple-sclerosis - Generation of suppressor T-cell lines | Authors: | ZHANG, JINGWU Schreurs, M MEDAER, Rob RAUS, Jef |
Issue Date: | 1992 | Publisher: | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Source: | CELLULAR IMMUNOLOGY, 139(1). p. 118-130 | Abstract: | Abstract Suppressor T cell (Ts) lines specific for myelin basic protein (MBP)-reactive helper T cell (Th) clones were generated from two patients with multiple sclerosis (MS) following a primary culture of peripheral blood mononuclear cells (PBMC) with MBP and cyclosporine A (CsA). These suppressor T cell lines were maintained in culture by alternate stimulation with MBP and antigen-presenting cells (APC). The Ts lines expressed preferentially the CD4 phenotype (5/6 Ts lines tested) and exhibited potent antigen-specific suppressor activity on the proliferation of MBP-specific Th clones and not on the T cell lines with other antigen specificity. For some Ts lines, a Ts-to-Th ratio of 1 was sufficient to inhibit the proliferation of MBP-specific T cells by 90%. The suppressor T cells obtained were weakly responsive to MBP and required the presence of the autologous PBMC for proliferation. Furthermore, proliferation of these suppressor T cell lines was restricted by HLA-DR molecules (for CD4+ Ts lines) and HLA class I (for a CD8+ Ts line). The suppressor T cell lines generated and the techniques described in this study may be helpful in our understanding of the events involved in the immune regulation in MS and other autoimmune diseases. | Notes: | DR L WILLEMS INST,DEPT IMMUNOL,UNIV CAMPUS,B-3590 DIEPENBEEK,BELGIUM. MULTIPLE SCLEROSIS CLIN & REHABIL CTR,OVERPELT,BELGIUM. LIMBURGS UNIV CENTRUM,B-3610 DIEPENBEEK,BELGIUM. | Document URI: | http://hdl.handle.net/1942/3854 | DOI: | 10.1016/0008-8749(92)90105-X | ISI #: | A1992GW21300011 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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