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http://hdl.handle.net/1942/3856
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DC Field | Value | Language |
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dc.contributor.author | CHIN, Y | - |
dc.contributor.author | JANSSENS, Jaak | - |
dc.contributor.author | SMEYERS, Els | - |
dc.contributor.author | BLEUS, Josianne | - |
dc.contributor.author | RAUS, Jef | - |
dc.contributor.author | ZHANG, JINGWU | - |
dc.date.accessioned | 2007-11-29T14:40:54Z | - |
dc.date.available | 2007-11-29T14:40:54Z | - |
dc.date.issued | 1992 | - |
dc.identifier.citation | ANTICANCER RESEARCH, 12(3). p. 733-736 | - |
dc.identifier.issn | 0250-7005 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3856 | - |
dc.description.abstract | Expansion of tumor infiltrating lymphocytes (TIL) in vitro is hampered by several factors, including a limited amount of lymphocytes obtained from different tumors, unknown target antigens and limited supply of antigen-presenting cells (APC) which are generally believed to be essential in the classical way of T cell stimulation and expansion. In approaching these difficulties, we have recently used surface-oxidized allogeneic PBL to stimulate the TIL periodically in the presence of a low dose of rIL-2 (200 IU/ml). TIL derived from 22 (out of 23) tumor specimens could be expanded with 20-10(7) fold increases over 6-16 weeks to a sufficient amount of 10(9)-10(11) cells for adoptive immunotherapy. In contrast, only 2-100 fold increase were observed in six tumor specimens (out of 23) when 200 IU/ml rIL-2 was used only. The phenotypes, autologous tumor reactivity and cytolytic capability of TIL propagated with surface-oxidized stimulators were similar to those expanded in the presence of IL-2 alone. These data suggest that expanding TIL with surface-modified stimulator cells could be a useful alternative method to obtain a large amount of tumor specific cytolytic T cells for clinical immunotherapeutic use, irrespective of tumor-antigen stimulation and MHC restriction. | - |
dc.language.iso | en | - |
dc.publisher | INT INST ANTICANCER RESEARCH | - |
dc.subject.other | TUMOR INFILTRATING LYMPHOCYTES; OXIDIZED PERIPHERAL BLOOD LYMPHOCYTES; INTERLEUKIN-2; CELL CULTURE | - |
dc.title | LARGE-SCALE EXPANSION OF HUMAN TUMOR INFILTRATING LYMPHOCYTES WITH SURFACE-MODIFIED STIMULATOR CELLS FOR ADOPTIVE IMMUNOTHERAPY | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 736 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 733 | - |
dc.identifier.volume | 12 | - |
local.format.pages | 4 | - |
dc.description.notes | DR L WILLEMS INST,DEPT CANC RES,B-3590 DIEPENBEEK,BELGIUM. LIMBURGS ONCOL CTR,B-3500 HASSELT,BELGIUM. LIMBURG STATE UNIV CTR,B-3590 DIEPENBEEK,BELGIUM. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.isi | A1992JB31600022 | - |
item.fulltext | No Fulltext | - |
item.accessRights | Closed Access | - |
item.contributor | CHIN, Y | - |
item.contributor | JANSSENS, Jaak | - |
item.contributor | SMEYERS, Els | - |
item.contributor | BLEUS, Josianne | - |
item.contributor | RAUS, Jef | - |
item.contributor | ZHANG, JINGWU | - |
item.fullcitation | CHIN, Y; JANSSENS, Jaak; SMEYERS, Els; BLEUS, Josianne; RAUS, Jef & ZHANG, JINGWU (1992) LARGE-SCALE EXPANSION OF HUMAN TUMOR INFILTRATING LYMPHOCYTES WITH SURFACE-MODIFIED STIMULATOR CELLS FOR ADOPTIVE IMMUNOTHERAPY. In: ANTICANCER RESEARCH, 12(3). p. 733-736. | - |
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