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http://hdl.handle.net/1942/38742
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DC Field | Value | Language |
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dc.contributor.author | Antonio, Luana Corsi | - |
dc.contributor.author | Ribovski, Lais | - |
dc.contributor.author | PINCELA LINS, Paula | - |
dc.contributor.author | Zucolotto, Valtencir | - |
dc.date.accessioned | 2022-10-19T11:27:31Z | - |
dc.date.available | 2022-10-19T11:27:31Z | - |
dc.date.issued | 2022 | - |
dc.date.submitted | 2022-10-06T09:20:30Z | - |
dc.identifier.citation | Journal of Materials Chemistry B, | - |
dc.identifier.uri | http://hdl.handle.net/1942/38742 | - |
dc.description.abstract | Polymeric nanocarriers (NCs) are efficient vehicles to prevent drug unspecific biodistribution and increase the drug amounts delivered to tumor tissues. However, some toxicological aspects of NCs still lack a comprehensive assessment, such as their effects on cellular processes that lead to toxicity. We evaluate the interaction of poly(lactic-co-glycolic acid) (PLGA) NCs prepared using dextran (Dex) and Pluronic (R)-F127 as stabilizing agents with myocardial cells (H9C2), breast adenocarcinoma cells (MCF-7) and macrophages (RAW 264.7) to address the effect of Dex in PLGA NC formulations. By an emulsion diffusion method, doxorubicin-loaded NCs were prepared with no Dex (PLGA-DOX), 1% (w/v) Dex (Dex1/PLGA-DOX) and 5% (w/v) Dex (Dex5/PLGA-DOX). Uptake analyses revealed a significant reduction in Dex5/PLGA-DOX NC uptake by H9C2 and MCF-7, as in the case of Dex1/PLGA-DOX NCs in the absence of in vitro protein corona, revealing an effect of dextran concentration on the formation of protein corona. RAW 264.7 cells presented a greater uptake of Dex5/PLGA-DOX NCs than the other NCs likely because of receptor mediated endocytosis, since C-type lectins like SIGN-R1, mannose receptors and scavenger receptor type 1 that are expressed in RAW 264.7 can mediate Dex uptake. Despite the lower uptake, Dex5/PLGA-DOX NCs promote the generation of reactive oxygen species and oxidative membrane damage in MCF-7 and H9C2 even though cellular metabolic activity assessed by MTT was comparable among all the NCs. Our results highlight the importance of an in-depth investigation of the NC-cell interaction considering additional mechanisms of damage apart from metabolic variations, as nanoparticle-induced damage is not limited to imbalance in metabolic processes, but also associated with other mechanisms, e.g., membrane and DNA damage. | - |
dc.description.sponsorship | LCA was supported by the Sa˜o Paulo Research Foundation (FAPESP) (Grant numbers 2020/02386-7) and by the Brazilian National Council for Scientific and Technological Development (CNPq) (project number 12085712017-0). LR was supported by the Coordenaça˜o de Aperfeiçoamento de Pessoal de Nı´vel Superior – Brasil (CAPES) – Finance Code 001. PMPL was supported by the Sa˜o Paulo Research Foundation (FAPESP) (Grant numbers 2017/21869-6 and 2020/00124-5) and by the Brazilian National Council for Scientific and Technological Development (CNPq) (project number 309943/2020-5 and 442690/2020-7). We acknowledge the facilities of the Central de Ana´lises Quı´micas Instrumentais (CAQI) of the Sa˜o Carlos Institute of Chemistry (IQSC) for the support and assistance in the use of the transmission electron microscope (FAPESP Grant number 2009/54216-9) and 400 MHz NMR. | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.rights | The Royal Society of Chemistry 2022. Open access | - |
dc.title | The amount of dextran in PLGA nanocarriers modulates protein corona and promotes cell membrane damage | - |
dc.type | Journal Contribution | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Ribovski, L (corresponding author), Univ Sao Paulo, Phys Inst Sao Carlos, Nanomed & Nanotoxicol Grp, CP 369, BR-13566590 Sao Carlos, SP, Brazil.; Ribovski, L (corresponding author), Univ Groningen, Univ Med Ctr Groningen, Dept Biomed Engn, Antonius Deusinglaan 1, NL-9713 AV Groningen, Netherlands. | - |
dc.description.notes | l.ribovski@umcg.nl | - |
local.publisher.place | THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.status | Early view | - |
dc.identifier.doi | 10.1039/d2tb01296k | - |
dc.identifier.pmid | 36155711 | - |
dc.identifier.isi | 000859238600001 | - |
dc.contributor.orcid | Corsi Antonio, Luana/0000-0001-9578-6277 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Antonio, Luana Corsi; Ribovski, Lais; Pincela Lins, Paula Maria; Zucolotto, Valtencir] Univ Sao Paulo, Phys Inst Sao Carlos, Nanomed & Nanotoxicol Grp, CP 369, BR-13566590 Sao Carlos, SP, Brazil. | - |
local.description.affiliation | [Ribovski, Lais] Univ Groningen, Univ Med Ctr Groningen, Dept Biomed Engn, Antonius Deusinglaan 1, NL-9713 AV Groningen, Netherlands. | - |
local.description.affiliation | [Pincela Lins, Paula Maria] Hasselt Univ, Fac Med & Life Sci, Biomed Res Inst BIOMED, Agoralaan, B-3590 Diepenbeek, Belgium. | - |
local.uhasselt.international | yes | - |
item.validation | ecoom 2023 | - |
item.fulltext | With Fulltext | - |
item.accessRights | Open Access | - |
item.fullcitation | Antonio, Luana Corsi; Ribovski, Lais; PINCELA LINS, Paula & Zucolotto, Valtencir (2022) The amount of dextran in PLGA nanocarriers modulates protein corona and promotes cell membrane damage. In: Journal of Materials Chemistry B,. | - |
item.contributor | Antonio, Luana Corsi | - |
item.contributor | Ribovski, Lais | - |
item.contributor | PINCELA LINS, Paula | - |
item.contributor | Zucolotto, Valtencir | - |
crisitem.journal.issn | 2050-750X | - |
crisitem.journal.eissn | 2050-7518 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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The amount of dextran in PLGA nanocarriers modulates protein corona and promotes cell membrane damage.pdf | Early view | 3.05 MB | Adobe PDF | View/Open |
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