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http://hdl.handle.net/1942/3914
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DC Field | Value | Language |
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dc.contributor.author | LEYSSENS, Luc | - |
dc.contributor.author | DRIESSEN, C | - |
dc.contributor.author | JACOBS, A | - |
dc.contributor.author | CZECH, Jan | - |
dc.contributor.author | RAUS, Jef | - |
dc.date.accessioned | 2007-11-29T15:47:46Z | - |
dc.date.available | 2007-11-29T15:47:46Z | - |
dc.date.issued | 1991 | - |
dc.identifier.citation | JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 564(2). p. 515-527 | - |
dc.identifier.issn | 0378-4347 | - |
dc.identifier.uri | http://hdl.handle.net/1942/3914 | - |
dc.description.abstract | A highly specific and sensitive method for the simultaneous detection of seven beta-2-receptor agonists in bovine liver homogenates and urine was developed. A 10-g amount of liver was homogenized and treated with Subtilisin A(R). The resulting enzymatic digest was extracted with tert.-butanol-ethyl acetate (3:7) and the crude extract was purified on a 6-ml Bakerbond(R) alumina neutral disposable extraction column. Subsequently, the hydrous eluate from the alumina column was buffered at pH 6 and loaded on top of a preconditioned 3-ml Bond-Elut Certify(R) column. Urine was buffered and loaded onto a 3-ml Certify column without pretreatment. The analytes were eluted with dichloromethane-isopropanol (8:2) containing 2% ammonia. The extract obtained was trimethylsilylated and analysed by gas chromatography-tandem mass spectrometry using multiple selected reaction monitoring. The limits of detection for the beta-2-receptor agonists evaluated were between 0.5 and 5 ppb. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Determination of beta-2-receptor agonists in bovine urine and liver by gas-chromatography tandem mass-spectrometry | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 527 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 515 | - |
dc.identifier.volume | 564 | - |
local.format.pages | 13 | - |
dc.description.notes | LEYSSENS, L, DR L WILLEMS INST,UNIV CAMPUS,B-3610 DIEPENBEEK,BELGIUM. | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/0378-4347(91)80520-M | - |
dc.identifier.isi | A1991FH21200016 | - |
item.fullcitation | LEYSSENS, Luc; DRIESSEN, C; JACOBS, A; CZECH, Jan & RAUS, Jef (1991) Determination of beta-2-receptor agonists in bovine urine and liver by gas-chromatography tandem mass-spectrometry. In: JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 564(2). p. 515-527. | - |
item.fulltext | No Fulltext | - |
item.contributor | LEYSSENS, Luc | - |
item.contributor | DRIESSEN, C | - |
item.contributor | JACOBS, A | - |
item.contributor | CZECH, Jan | - |
item.contributor | RAUS, Jef | - |
item.accessRights | Closed Access | - |
Appears in Collections: | Research publications |
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