Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/39715
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dc.contributor.authorDIOUF, David-
dc.contributor.authorVitale, Maria Rosaria-
dc.contributor.authorZoeller, Johanna Eva Maria-
dc.contributor.authorPineau, Ana -Magdalena-
dc.contributor.authorKlopocki, Eva-
dc.contributor.authorHamann, Catharina-
dc.contributor.authorZiegler, Georg Christoph-
dc.contributor.authorVANMIERLO, Tim-
dc.contributor.authorVan den Hove, Daniel-
dc.contributor.authorLesch, Klaus-Peter-
dc.date.accessioned2023-03-16T07:58:36Z-
dc.date.available2023-03-16T07:58:36Z-
dc.date.issued2023-
dc.date.submitted2023-03-15T14:21:05Z-
dc.identifier.citationStem Cell Research, 67 (Art N° 103038)-
dc.identifier.urihttp://hdl.handle.net/1942/39715-
dc.description.abstractFibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.-
dc.description.sponsorshipThis work was supported by ERA-NET NEURON under Grant No. 01EW1902 (DECODE!), the Horizon 2020 Research and Innovation Programme under Grant No. 728018 (Eat2beNICE), Grant No. 953327 (Serotonin and Beyond), the Horizon Europe Research and Innovation Programme under Grant No. EU 101086453 (Aqua-Synapse), the DFG (Grant No. 413657723, Clinician Scientist-Program UNION CVD), and the University of Würzburg in the funding program Open Access Publishing. We thank Julia Merk, Gabriela Ortega, and Nicole Schraud for excellent technical assistance.-
dc.language.isoen-
dc.publisherELSEVIER-
dc.rights2023 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).-
dc.subject.otherHumans-
dc.subject.otherGene Editing-
dc.subject.otherCRISPR-Cas Systems-
dc.subject.otherCell Differentiation-
dc.subject.otherCell Line-
dc.subject.otherInduced Pluripotent Stem Cells-
dc.titleGeneration of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing-
dc.typeJournal Contribution-
dc.identifier.volume67-
local.bibliographicCitation.jcatA1-
dc.description.notesDiouf, D; Lesch, KP (corresponding author), Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany.-
dc.description.notesDiouf_D@ukw.de; kplesch@mail.uni-wuerzburg.de-
local.publisher.placeRADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr103038-
local.type.programmeH2020-
local.type.programmehorizonEurope-
local.relation.h2020728018-
dc.identifier.doi10.1016/j.scr.2023.103038-
dc.identifier.pmid36746102-
dc.identifier.isi000930528500001-
dc.contributor.orcidVitale, Maria Rosaria/0000-0002-1108-0523; Diouf,-
dc.contributor.orcidDavid/0000-0002-1728-5319-
local.provider.typewosris-
local.description.affiliation[Diouf, David; Vitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Pineau, Ana -Magdalena; Hamann, Catharina; Ziegler, Georg Christoph; Lesch, Klaus-Peter] Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Wurzburg, Germany.-
local.description.affiliation[Klopocki, Eva] Univ Wurzburg, Inst Human Genet, Bioctr, Wurzburg, Germany.-
local.description.affiliation[Ziegler, Georg Christoph] Univ Hosp Wurzburg, Ctr Mental Hlth, Dept Psychiat Psychosomat & Psychotherapy, Wurzburg, Germany.-
local.description.affiliation[Vanmierlo, Tim; Van den Hove, Daniel; Lesch, Klaus-Peter] Maastricht Univ, Sch Mental Hlth & Neurosci MHeNs, Dept Psychiat & Neuropsychol, Maastricht, Netherlands.-
local.description.affiliation[Vanmierlo, Tim] Hasselt Univ, Biomed Res Inst, Neuroimmune Connect & Repair Lab, Hasselt, Belgium.-
local.description.affiliation[Diouf, David; Lesch, Klaus-Peter] Univ Hosp Wurzburg, Ctr Mental Hlth, Div Mol Psychiat, Margarete Hoppel Pl 1, D-97080 Wurzburg, Germany.-
local.uhasselt.internationalyes-
local.relation.horizonEurope101086453-
item.fullcitationDIOUF, David; Vitale, Maria Rosaria; Zoeller, Johanna Eva Maria; Pineau, Ana -Magdalena; Klopocki, Eva; Hamann, Catharina; Ziegler, Georg Christoph; VANMIERLO, Tim; Van den Hove, Daniel & Lesch, Klaus-Peter (2023) Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing. In: Stem Cell Research, 67 (Art N° 103038).-
item.fulltextWith Fulltext-
item.contributorDIOUF, David-
item.contributorVitale, Maria Rosaria-
item.contributorZoeller, Johanna Eva Maria-
item.contributorPineau, Ana -Magdalena-
item.contributorKlopocki, Eva-
item.contributorHamann, Catharina-
item.contributorZiegler, Georg Christoph-
item.contributorVANMIERLO, Tim-
item.contributorVan den Hove, Daniel-
item.contributorLesch, Klaus-Peter-
item.accessRightsOpen Access-
crisitem.journal.issn1873-5061-
crisitem.journal.eissn1876-7753-
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