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Title: | Isolation, purification, and metal-induced gelation of released polysaccharides from spent culture medium of Arthrospira | Authors: | Cuellar-Bermudez, Sara P. BLEUS, Dries Mees, Maarten A. Struyf, Tom LAMA, Sanjaya VANDAMME, Dries ADRIAENSENS, Peter Scherrers, Roger Thielemans, Wim Van den Ende, Wim Wattiez, Ruddy Muylaert, Koenraad |
Issue Date: | 2023 | Publisher: | ELSEVIER | Source: | Algal Research-Biomass Biofuels and Bioproducts, 70 (Art N° 103010) | Abstract: | Cyanobacteria release polysaccharides that accumulate in the culture medium. In this study, released polysaccharides were isolated from the spent culture medium of an Arthrospira farm with a carbohydrate concentration of 136 mg L−1. The polysaccharides were concentrated 5.2 times by ultrafiltration using a 50 kDa polysulphone membrane and purified (desalting, protein removal). The polysaccharides were rich in uronic acid (159 mg g−1), had a low sulfate content (2.3 mg g−1), and contained 9 different monosaccharides, with 2-deoxy-d-galactose (27.9 %), fucose (22.7 %), glucose (22.0 %), and glucuronic acid (17.1 %) being dominant. A 1 % w/v polysaccharide concentrate formed stable gel beads in Al3+ and Fe3+ solutions. Rheological characterization of the gel revealed stable storage (G' = 38 Pa) and loss modulus (G" = 6 Pa) over a wide frequency range. This study demonstrates the feasibility to recover polysaccharides from Arthrospira spent culture medium and the potential to use these polysaccharides in food or biotechnological applications. | Keywords: | Spirulina;Limnospira;Extracellular polysaccharides;Ultrafiltration;Gel rheology;Hydrogel | Document URI: | http://hdl.handle.net/1942/39880 | ISSN: | 2211-9264 | e-ISSN: | 2211-9264 | DOI: | 10.1016/j.algal.2023.103010 | ISI #: | 000948808400001 | Rights: | 2023PublishedbyElsevierB.V | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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