Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/40010
Title: | Multiplex Analysis of Cerebrospinal Fluid and Serum Exosomes MicroRNAs of Untreated Relapsing Remitting Multiple Sclerosis (RRMS) and Proposing Noninvasive Diagnostic Biomarkers | Authors: | Mohammadinasr, Mina Montazersaheb, Soheila Molavi, Ommoleila KAHROBA, Houman Talebi, Mahnaz Ayromlou, Hormoz Hejazi, Mohammad Saeid |
Issue Date: | 2023 | Publisher: | HUMANA PRESS INC | Source: | NEUROMOLECULAR MEDICINE, 25, p.402–414 | Abstract: | Exosomal microRNAs (miRNAs) are emerging diagnostic biomarkers for neurodegenerative diseases. In this study, we aimed to detect relapsing-remitting multiple sclerosis (RRMS)-specific miRNAs in cerebrospinal fluid (CSF) and serum exosomes with diagnostic potential. One ml of CSF and serum sample were collected from each of the 30 untreated RRMS patients and healthy controls (HCs). A panel of 18 miRNAs affecting inflammatory responses was applied, and qRT-PCR was conducted to detect differentially expressed exosomal miRNAs in CSF and serum of RRMS patients. We identified that 17 out of 18 miRNAs displayed different patterns in RRMS patients compared to HCs. Let-7 g-5p, miR-18a-5p, miR-145-5p, and miR-374a-5p with dual pro-inflammatory and anti-inflammatory actions and miR-150-5p and miR-342-3p with anti-inflammatory action were significantly upregulated in both CSF and serum-derived exosomes of RRMS patients compared to corresponding HCs. Additionally, anti-inflammatory miR-132-5p and pro-inflammatory miR-320a-5p were significantly downregulated in both CSF and serum-derived exosomes of RRMS patients compared to HCs. Ten of 18 miRNAs were differentially expressed in CSF and serum exosomes of the patients. Furthermore, miR-15a-5p, miR-19b-3p, and miR-432-5p were upregulated, and miR-17-5p was downregulated only in CSF exosomes. Interestingly, U6 housekeeping gene was differentially expressed in CSF and serum exosomes, in both RRMS and HCs. As the first report describing CSF exosomal miRNAs expression profile compared to that of serum exosomes in untreated RRMS patients, we showed that CSF and serum exosomes are not identical in terms of biological compounds and display different patterns in miRNAs and U6 expression. | Notes: | Hejazi, MS (corresponding author), Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Mol Med, Tabriz, Iran.; Hejazi, MS (corresponding author), Tabriz Univ Med Sci, Mol Med Res Ctr, Tabriz, Iran.; Hejazi, MS (corresponding author), Tabriz Univ Med Sci, Fac Pharm, Dept Pharmaceut Biotechnol, Tabriz, Iran. msaeidhejazi@yahoo.com |
Keywords: | Relapsing Remitting MS (RRMS);Exosome;MicroRNA (miRNA);Cerebrospinal Fluid (CSF);U6;Serum | Document URI: | http://hdl.handle.net/1942/40010 | ISSN: | 1535-1084 | e-ISSN: | 1559-1174 | DOI: | 10.1007/s12017-023-08744-3 | ISI #: | 000963728900001 | Rights: | The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023 | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
auteursversie.pdf | Peer-reviewed author version | 1.23 MB | Adobe PDF | View/Open |
s12017-023-08744-3.pdf Restricted Access | Published version | 1.47 MB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.