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http://hdl.handle.net/1942/40047
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DC Field | Value | Language |
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dc.contributor.author | Schonkeren, Simone L. L. | - |
dc.contributor.author | Seeldrayers, Saskia | - |
dc.contributor.author | THIJSSEN, Meike | - |
dc.contributor.author | BOESMANS, Werend | - |
dc.contributor.author | Langen, Ramon C. J. | - |
dc.contributor.author | Melotte, Veerle | - |
dc.date.accessioned | 2023-05-09T08:51:49Z | - |
dc.date.available | 2023-05-09T08:51:49Z | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2023-05-03T14:45:32Z | - |
dc.identifier.citation | NEUROGASTROENTEROLOGY AND MOTILITY, (Art N° e14586) | - |
dc.identifier.uri | http://hdl.handle.net/1942/40047 | - |
dc.description.abstract | BackgroundGastrointestinal motility measurements in mice are currently performed under suboptimal conditions, as these nocturnal animals are measured during light conditions. In addition, other stressors, like individual housing, placement in a new cage during observation, and lack of bedding and cage enrichment cause animal discomfort and might contribute to higher variability. Here we aimed to develop a refined method of the widely-used whole-gut transit assay. MethodsWildtype mice (N = 24) were subjected to the standard or refined whole-gut transit assay, either with or without a standardized slowing in gastrointestinal motility induced by loperamide. The standard assay consisted of a gavage with carmine red, observation during the light period and individual housing in a new cage without cage enrichment. For the refined whole-gut transit assay, mice were gavaged with UV-fluorescent DETEX (R), observed during the dark period, while pairwise housed in their home cage with cage enrichment. Time until excretion of the first colored fecal pellet was assessed, and pellets were collected to assess number, weight, and water content. Key ResultsThe DETEX (R)-containing pellets were UV-detectable, allowing to measure the mice in their active period in the dark. The refined method caused less variation (20.8% and 16.0%) compared to the standard method (29.0% and 21.7%). Fecal pellet number, weight, and water content was significantly different between the standard and refined method. Conclusions & InferencesThis refined whole-gut transit assay provides a reliable approach to measure whole-gut transit time in mice in a more physiological context, with reduced variability compared to the standard method. | - |
dc.description.sponsorship | This work is supported by The Netherlands Organization for Scientific Research (NWO) Veni grant, grant number 016.186.124 (VM). We would like to acknowledge the help of Kim Smits with the statistical analysis of the data in this manuscript. | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.rights | 2023 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited | - |
dc.subject.other | carmine red | - |
dc.subject.other | DETEX | - |
dc.subject.other | gastrointestinal transit | - |
dc.subject.other | intestinal motility | - |
dc.subject.other | loperamide | - |
dc.title | An optimization and refinement of the whole-gut transit assay in mice | - |
dc.type | Journal Contribution | - |
local.format.pages | 6 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Melotte, V (corresponding author), Maastricht Univ, Med Ctr, GROW Sch Oncol & Reprod, Dept Pathol, POB 616, NL-6200 MD Maastricht, Netherlands. | - |
dc.description.notes | veerle.melotte@maastrichtuniversity.nl | - |
local.publisher.place | 111 RIVER ST, HOBOKEN 07030-5774, NJ USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | e14586 | - |
dc.identifier.doi | 10.1111/nmo.14586 | - |
dc.identifier.pmid | 37010851 | - |
dc.identifier.isi | 000961706200001 | - |
dc.contributor.orcid | melotte, veerle/0000-0002-9459-123X; Boesmans, | - |
dc.contributor.orcid | Werend/0000-0002-2426-0451; Seeldrayers, Saskia/0000-0001-6191-359X; | - |
dc.contributor.orcid | Thijssen, Meike/0000-0001-7073-2096 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Schonkeren, Simone L. L.; Thijssen, Meike S. S.; Boesmans, Werend; Melotte, Veerle] Maastricht Univ, Med Ctr, GROW Sch Oncol & Reprod, Dept Pathol, POB 616, NL-6200 MD Maastricht, Netherlands. | - |
local.description.affiliation | [Seeldrayers, Saskia] Maastricht Univ, Cent Anim Facil, Maastricht, Netherlands. | - |
local.description.affiliation | [Seeldrayers, Saskia; Langen, Ramon C. J.] Maastricht Univ, Anim Welf Body, Maastricht, Netherlands. | - |
local.description.affiliation | [Thijssen, Meike S. S.; Boesmans, Werend] Hasselt Univ, Biomed Res Inst BIOMED, Hasselt, Belgium. | - |
local.description.affiliation | [Langen, Ramon C. J.] Maastricht Univ, NUTRIM Sch Nutr Toxicol & Metab, Med Ctr, Dept Resp Med, Maastricht, Netherlands. | - |
local.description.affiliation | [Melotte, Veerle] Erasmus MC, Dept Clin Genet, Rotterdam, Netherlands. | - |
local.uhasselt.international | yes | - |
item.accessRights | Open Access | - |
item.fullcitation | Schonkeren, Simone L. L.; Seeldrayers, Saskia; THIJSSEN, Meike; BOESMANS, Werend; Langen, Ramon C. J. & Melotte, Veerle (2023) An optimization and refinement of the whole-gut transit assay in mice. In: NEUROGASTROENTEROLOGY AND MOTILITY, (Art N° e14586). | - |
item.fulltext | With Fulltext | - |
item.contributor | Schonkeren, Simone L. L. | - |
item.contributor | Seeldrayers, Saskia | - |
item.contributor | THIJSSEN, Meike | - |
item.contributor | BOESMANS, Werend | - |
item.contributor | Langen, Ramon C. J. | - |
item.contributor | Melotte, Veerle | - |
crisitem.journal.issn | 1350-1925 | - |
crisitem.journal.eissn | 1365-2982 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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An optimization and refinement of the whole‐gut transit assay in mice.pdf | Published version | 1.54 MB | Adobe PDF | View/Open |
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