Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/40106
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dc.contributor.authorHERMANS, Florian-
dc.contributor.authorHEMERYCK, Lara-
dc.contributor.authorBueds, Celine-
dc.contributor.authorTorres Pereiro, Marc-
dc.contributor.authorHASEVOETS, Steffie-
dc.contributor.authorKobayashi, Hiroto-
dc.contributor.authorLambrechts, Diether-
dc.contributor.authorLAMBRICHTS, Ivo-
dc.contributor.authorBRONCKAERS, Annelies-
dc.contributor.authorVankelecom, Hugo-
dc.date.accessioned2023-05-15T09:10:56Z-
dc.date.available2023-05-15T09:10:56Z-
dc.date.issued2023-
dc.date.submitted2023-05-15T08:09:34Z-
dc.identifier.citationStem Cell Reports, 18 (5) , p. 1166 -1181-
dc.identifier.issn2213-6711-
dc.identifier.urihttp://hdl.handle.net/1942/40106-
dc.description.abstractOrganoid models provide powerful tools to study tissue biology and development in a dish. Presently, organoids have not yet been developed from mouse tooth. Here, we established tooth organoids (TOs) from early-postnatal mouse molar and incisor, which are long-term expandable, express dental epithelium stem cell (DESC) markers, and recapitulate key properties of the dental epithelium in a tooth-type-specific manner. TOs display in vitro differentiation capacity toward ameloblast-resembling cells, even more pronounced in assembloids in which dental mesenchymal (pulp) stem cells are combined with the organoid DESCs. Single-cell transcriptomics supports this developmental potential and reveals co-differentiation into junctional epithelium- and odontoblast-/cementoblast-like cells in the assembloids. Finally, TOs survive and show ameloblast-resembling differentiation also in vivo. The developed organoid models provide new tools to study mouse tooth-type-specific biology and development and gain deeper molecular and functional insights that may eventually help to achieve future human biological tooth repair and replacement.-
dc.description.sponsorshipWe are grateful to Evelyne Van Kerckhove (UHasselt), Marc Jans (UHasselt), Jeanine Santermans (UHasselt), and Veerle Vanslembrouck (KU Leuven) for valuable technical help. We thank Dr. Diether Lambrechts’ group (VIB,KULeuven) for technical assistance in 103 Genomics. Computational resources for transcriptome analyses were provided by the ‘‘Vlaams Supercomputer Centrum’’ (VSC), managed by the Fund for Scientific Research (FWO), Flanders (Belgium).We are also grateful to the ImagingCore (VIB,KULeuven) and the CIC (KU Leuven) for use ofmicroscopes and the Center for Brain & Disease Research (CBD) Histology unit (VIB, KU Leuven) for use of histology equipment. We acknowledge the use of the TEMplatforms at VIB-KU Leuven, UHasselt, and Tohoku University. The authors also thank Dr. Adrian Ranga (KU Leuven), Dr. Ronald Driesen(UHasselt), and other non-coauthor members of the Laboratory of Tissue Plasticity in Health and Disease (KU Leuven) for their input. Certain figures were created using BioRender.com or GraphPad Prism (v9.3.1) for macOS. This work was supported by grants from KU Leuven (Research Fund) and Fund for Scientific Research (FWO) Flanders. L.H. was an FWO PhD Fellow (1S84718N). C.B. is an FWO PhD Fellow (1129323N), while F.H. was supported by an FWO project grant (G061819FWO). Use of the Zeiss LSM 780 – SP Mai Tai HP DS is supported by Hercules AKUL/11/37 and FWO G.0929.15 funding to Dr. Pieter Vanden Berghe (CIC, KU Leuven), while the application of the JEOL FLASH 1400 TEM was supported by Hercules FWO funding I000220N to I.L.-
dc.language.isoen-
dc.publisherCell Press-
dc.rights2023 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).-
dc.subject.otherameloblasts-
dc.subject.otherassembloids-
dc.subject.otherdental epithelium-
dc.subject.otherincisors-
dc.subject.othermolars-
dc.subject.otherorganoids-
dc.subject.othersingle-cell RNA-sequencing-
dc.subject.otherstem cells-
dc.subject.othertooth development-
dc.subject.otherAnimals-
dc.subject.otherMice-
dc.subject.otherHumans-
dc.subject.otherMolar-
dc.subject.otherCell Differentiation-
dc.subject.otherOrganoids-
dc.subject.otherBiology-
dc.subject.otherIncisor-
dc.subject.otherAmeloblasts-
dc.titleOrganoids from mouse molar and incisor as new tools to study tooth-specific biology and development-
dc.typeJournal Contribution-
dc.identifier.epage1181-
dc.identifier.issue5-
dc.identifier.spage1166-
dc.identifier.volume18-
local.bibliographicCitation.jcatA1-
local.publisher.place50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.type.programmeVSC-
dc.identifier.doi10.1016/j.stemcr.2023.03.011-
dc.identifier.pmid37084723-
dc.identifier.isi001017408400001-
dc.identifier.urlhttps://www.cell.com/stem-cell-reports/fulltext/S2213-6711(23)00099-1-
dc.identifier.eissn2213-6711-
local.provider.typePubMed-
local.dataset.doi10.17632/2kskdknngb.1-
local.dataset.urlhttps://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-12557-
local.dataset.urlhttps://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-12544-
local.uhasselt.internationalyes-
item.fulltextWith Fulltext-
item.fullcitationHERMANS, Florian; HEMERYCK, Lara; Bueds, Celine; Torres Pereiro, Marc; HASEVOETS, Steffie; Kobayashi, Hiroto; Lambrechts, Diether; LAMBRICHTS, Ivo; BRONCKAERS, Annelies & Vankelecom, Hugo (2023) Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development. In: Stem Cell Reports, 18 (5) , p. 1166 -1181.-
item.accessRightsOpen Access-
item.contributorHERMANS, Florian-
item.contributorHEMERYCK, Lara-
item.contributorBueds, Celine-
item.contributorTorres Pereiro, Marc-
item.contributorHASEVOETS, Steffie-
item.contributorKobayashi, Hiroto-
item.contributorLambrechts, Diether-
item.contributorLAMBRICHTS, Ivo-
item.contributorBRONCKAERS, Annelies-
item.contributorVankelecom, Hugo-
crisitem.journal.issn2213-6711-
crisitem.journal.eissn2213-6711-
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