Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/4055
Title: Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations
Authors: MULLER, Ludo 
Craciun, A. R.
RUYTINX, Joske 
LAMBAERTS, Marc 
Verbruggen, N.
VANGRONSVELD, Jaco 
COLPAERT, Jan 
Issue Date: 2007
Publisher: SPRINGER
Source: MYCORRHIZA, 17(7). p. 571-580
Abstract: Complementary DNA (cDNA)-amplified fragment-length polymorphism (AFLP) was applied to analyze transcript profiles of a Zn-tolerant and a Zn-sensitive isolate of the ectomycorrhizal basidiomycete Suillus luteus, both cultured with and without increased external zinc concentrations. From the obtained transcript profiles that covered approximately 2% of the total expected complement of genes in S. luteus, 144 nonredundant, differentially expressed transcript-derived fragments (TDFs), falling in different classes of expression pattern, were isolated and sequenced. Thirty-six of the represented genes showed homology to function-known genes, whereas 6 matched unknown protein coding sequences, and 102 were possibly novel. Although relatively few TDFs were found to be responsive to the different zinc treatments, their modulated expression levels may suggest a different transcriptional response to zinc treatments in both isolates. Among the identified genes that could be related to heavy-metal detoxification or the tolerance trait were genes encoding for homologues of a heat-shock protein, a putative metal transporter, a hydrophobin, and several proteins involved in ubiquitin-dependent proteolysis.
Notes: Hasselt Univ, Environm Biol Grp, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. Univ Libre Bruxelles, Lab Physiol & Genet Mol Plantes, B-1050 Brussels, Belgium. Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA.COLPAERT, JV, Hasselt Univ, Environm Biol Grp, Ctr Environm Sci, Gebouw D, B-3590 Diepenbeek, Belgium.jan.colpaert@uhasselt.be
Keywords: Suillus luteus; Zn tolerance; Zn toxicity; gene expression profiling; cDNA–AFLP;Suillus luteus; Zn tolerance; Zn toxicity; gene expression profiling; cDNA-AFLP
Document URI: http://hdl.handle.net/1942/4055
ISSN: 0940-6360
e-ISSN: 1432-1890
DOI: 10.1007/s00572-007-0134-5
ISI #: 000249794100003
Rights: © Springer-Verlag 2007.
Category: A1
Type: Journal Contribution
Validations: ecoom 2008
Appears in Collections:Research publications

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