Please use this identifier to cite or link to this item:
|Title:||Gene expression profiling of a Zn-tolerant and a Zn-sensitive Suillus luteus isolate exposed to increased external zinc concentrations||Authors:||MULLER, Ludo
Craciun, A. R.
|Issue Date:||2007||Publisher:||SPRINGER||Source:||MYCORRHIZA, 17(7). p. 571-580||Abstract:||Complementary DNA (cDNA)-amplified fragment-length polymorphism (AFLP) was applied to analyze transcript profiles of a Zn-tolerant and a Zn-sensitive isolate of the ectomycorrhizal basidiomycete Suillus luteus, both cultured with and without increased external zinc concentrations. From the obtained transcript profiles that covered approximately 2% of the total expected complement of genes in S. luteus, 144 nonredundant, differentially expressed transcript-derived fragments (TDFs), falling in different classes of expression pattern, were isolated and sequenced. Thirty-six of the represented genes showed homology to function-known genes, whereas 6 matched unknown protein coding sequences, and 102 were possibly novel. Although relatively few TDFs were found to be responsive to the different zinc treatments, their modulated expression levels may suggest a different transcriptional response to zinc treatments in both isolates. Among the identified genes that could be related to heavy-metal detoxification or the tolerance trait were genes encoding for homologues of a heat-shock protein, a putative metal transporter, a hydrophobin, and several proteins involved in ubiquitin-dependent proteolysis.||Notes:||Hasselt Univ, Environm Biol Grp, Ctr Environm Sci, B-3590 Diepenbeek, Belgium. Univ Libre Bruxelles, Lab Physiol & Genet Mol Plantes, B-1050 Brussels, Belgium. Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA.COLPAERT, JV, Hasselt Univ, Environm Biol Grp, Ctr Environm Sci, Gebouw D, B-3590 Diepenbeek, Belgium.email@example.com||Keywords:||Suillus luteus; Zn tolerance; Zn toxicity; gene expression profiling; cDNA–AFLP;Suillus luteus; Zn tolerance; Zn toxicity; gene expression profiling; cDNA-AFLP||Document URI:||http://hdl.handle.net/1942/4055||ISSN:||0940-6360||e-ISSN:||1432-1890||DOI:||10.1007/s00572-007-0134-5||ISI #:||000249794100003||Rights:||© Springer-Verlag 2007.||Category:||A1||Type:||Journal Contribution||Validations:||ecoom 2008|
|Appears in Collections:||Research publications|
Show full item record
Files in This Item:
|291.23 kB||Adobe PDF||View/Open Request a copy|
checked on Sep 5, 2020
WEB OF SCIENCETM
checked on May 29, 2022
checked on May 28, 2022
checked on May 28, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.