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http://hdl.handle.net/1942/4059
Title: | An improved sample preparation for an LC method used in the age estimation based on aspartic acid racemization from human dentin | Authors: | Yekkala, Raja Meers, Carine Hoogmartens, Jos LAMBRICHTS, Ivo WILLEMS, Guy Van Schepdael, Ann |
Issue Date: | 2007 | Publisher: | WILEY-V C H VERLAG GMBH | Source: | JOURNAL OF SEPARATION SCIENCE, 30(1). p. 118-121 | Abstract: | The determination of age on the basis of aspartic acid (Asp) racemization in teeth is one of the most reliable and accurate methods to date. In this paper, the usefulness of HPLC coupled with fluorescence detection for determination of Asp racemization was evaluated. A modified sample preparation is proposed for better stability of o-phthaldialdehyde-N-acetyl-L-cysteine derivatives of D/L-Asp (due to the instability below pH 7). To ensure the accuracy of the method, the validation parameters' specificity, precision, linearity, and LOD were determined. Three dentin samples of premolar teeth, extracted from living individuals (bucco-lingual longitudinal sections of 1 mm thickness), were analyzed and quantitative results are discussed. | Notes: | Katholieke Univ Leuven, Lab Pharmaceut Anal, B-3000 Louvain, Belgium. Katholieke Univ Leuven, Dept Forens Odontol, Sch Dent Oral Pathol & Maxillofacial Surg, Louvain, Belgium. Univ Hasselt, Lab Histol, Biomed Res Inst, Diepenbeek, Belgium.Van Schepdael, A, Katholieke Univ Leuven, Lab Pharmaceut Chem & Drug Anal, O&N 2,Postbus 923,Herestr 49, B-3000 Louvain, Belgium.ann.vanschepdael@pharm.kuleuven.be | Keywords: | age estimation; dentin; D-aspartic acid; racemization; teeth | Document URI: | http://hdl.handle.net/1942/4059 | ISSN: | 1615-9306 | e-ISSN: | 1615-9314 | DOI: | 10.1002/jssc.200600191 | ISI #: | 000243776600018 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2008 |
Appears in Collections: | Research publications |
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