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http://hdl.handle.net/1942/4070
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DC Field | Value | Language |
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dc.contributor.author | DUMONT, Debora | - |
dc.contributor.author | Ceuppens, Ruben | - |
dc.contributor.author | Van Brussel, Leen | - |
dc.contributor.author | DANIELS, Ruth | - |
dc.contributor.author | NOBEN, Jean-Paul | - |
dc.contributor.author | Van de Plas, Babs | - |
dc.contributor.author | VERHAERT, Peter | - |
dc.contributor.author | Van der Gucht, Estel | - |
dc.contributor.author | Clerens, Stefan | - |
dc.contributor.author | Arckens, Lutgarde | - |
dc.contributor.author | ROBBEN, Johan | - |
dc.date.accessioned | 2007-12-10T09:38:32Z | - |
dc.date.available | 2007-12-10T09:38:32Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 260(2-3). p. 185-194 | - |
dc.identifier.issn | 1387-3806 | - |
dc.identifier.uri | http://hdl.handle.net/1942/4070 | - |
dc.description.abstract | One of the newly developed imaging mass spectrometry (IMS) technologies utilizes matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to map proteins in thin tissue sections. In this study, we evaluated the power of MALDI IMS as we developed it in our (Bruker) MALDI TOF (Reflex IV) and TOF-TOF (Ultraflex II) systems to study myelin patterns in the mouse central nervous system under normal and pathological conditions. MALDI IMS was applied to assess myelin basic protein (MBP) isoform-specific profiles in different regions throughout the mouse brain. The distribution of ions of m/z 14,144 and 18,447 displayed a striking resemblance with white matter histology and were identified as MBP isoform 8 and 5, respectively. In addition, we demonstrated a significant reduction of the MBP-8 peak intensity upon MALDI IMS analysis of focal ethidium bromide-induced demyelinated brain areas. Our MS images were validated by immunohistochemistry using MBP antibodies. This study underscores the potential of MALDI IMS to study the contribution of MBP to demyelinating diseases. (C) 2006 Elsevier B.V. All rights reserved. | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject.other | MALDI IMS; mouse; brain; myclin basic protein; histology | - |
dc.title | Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 194 | - |
dc.identifier.issue | 2-3 | - |
dc.identifier.spage | 185 | - |
dc.identifier.volume | 260 | - |
local.format.pages | 10 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Katholieke Univ Leuven, Lab Neuroplast & Neuroproteom, B-3000 Louvain, Belgium. Hasselt Univ, Biomed Res Inst Biomed & Transnatl Univ Limburg, Sch Life Sci, Diepenbeek, Belgium. Delft Univ Technol, Lab Analyt Biotechnol, Delft, Netherlands.Arckens, L, Katholieke Univ Leuven, Lab Neuroplast & Neuroproteom, B-3000 Louvain, Belgium.ruben.ceuppens@bio.kuleuven.be debora.dumont@uhasselt.be leen.vanbrussel@bio.kuleuven.be babs.vandeplas@bio.kuleuven.be ruth.daniels@uhasselt.be jeanpaul.noben@uhasselt.be peter.verhaert@udelft.nl estel.vandergucht@bio.kuleuven.be johan.robben@uhasselt.be stefan.clerens@canesis.com Lut.arckens@bio.kuleuven.be | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.bibliographicCitation.oldjcat | A1 | - |
dc.identifier.doi | 10.1016/j.ijms.2006.09.007 | - |
dc.identifier.isi | 000243743500013 | - |
item.fulltext | No Fulltext | - |
item.accessRights | Closed Access | - |
item.contributor | DUMONT, Debora | - |
item.contributor | Ceuppens, Ruben | - |
item.contributor | Van Brussel, Leen | - |
item.contributor | DANIELS, Ruth | - |
item.contributor | NOBEN, Jean-Paul | - |
item.contributor | Van de Plas, Babs | - |
item.contributor | VERHAERT, Peter | - |
item.contributor | Van der Gucht, Estel | - |
item.contributor | Clerens, Stefan | - |
item.contributor | Arckens, Lutgarde | - |
item.contributor | ROBBEN, Johan | - |
item.fullcitation | DUMONT, Debora; Ceuppens, Ruben; Van Brussel, Leen; DANIELS, Ruth; NOBEN, Jean-Paul; Van de Plas, Babs; VERHAERT, Peter; Van der Gucht, Estel; Clerens, Stefan; Arckens, Lutgarde & ROBBEN, Johan (2007) Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry. In: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 260(2-3). p. 185-194. | - |
item.validation | ecoom 2008 | - |
crisitem.journal.issn | 1387-3806 | - |
crisitem.journal.eissn | 1873-2798 | - |
Appears in Collections: | Research publications |
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