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Title: | Neuropeptide imaging on an LTQ with vMALDI source: The complete 'all-in-one' peptidome analysis | Authors: | VERHAERT, Peter Conaway, Maria C. Prieto Pekar, Tonya M. Miller, Ken |
Issue Date: | 2007 | Publisher: | ELSEVIER SCIENCE BV | Source: | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 260(2-3). p. 177-184 | Abstract: | Direct tissue imaging was performed on dissected insect tissue using a MALDI ion trap to visualize endogenous neuropeptides. Coupling tissue imaging to tandem MSn allows for the identification of previously known species and the ability to identify new ones by de novo sequencing, as searchable databases for insects are sparse. Direct tissue imaging is an attractive technique for the study of neuropeptides as minimal sample preparation is required prior to mass spectrometry. We successfully identified neuropeptides present in the corpora cardiaca and allata of Acheta domesticus (the house cricket). Diagnostic fragments at low m/z were used to distinguish between lipids and neuropeptides. The distribution of peptides appears to be more differentially localized than that of phospholipids, which seem to be more evenly distributed within the tissue. (C) 2006 Elsevier B.V. All rights reserved. | Notes: | Delft Univ Technol, Dept Biotechnol, Lab Analyt Biotechnol, NL-2628 BC Delft, Netherlands. Univ Hasselt, Biomed Res Ctr, Hasselt, Belgium. Univ Liege, Mass Spectrometry Lab, B-4000 Liege, Belgium. Flemish Interuniv Inst Biotechnol, Mol Cell Biol Lab, Louvain, Belgium. Thermo Electron Corp, San Jose, CA USA.VERHAERT, PD, Delft Univ Technol, Dept Biotechnol, Lab Analyt Biotechnol, Julianalaan 67, NL-2628 BC Delft, Netherlands.p.d.e.m.verhaert@tudelft.nl | Keywords: | MALDI MS imaging; ion trap; MSn; neuropeptide | Document URI: | http://hdl.handle.net/1942/4075 | ISSN: | 1387-3806 | e-ISSN: | 1873-2798 | DOI: | 10.1016/j.ijms.2006.11.008 | ISI #: | 000243743500012 | Category: | A1 | Type: | Journal Contribution | Validations: | ecoom 2008 |
Appears in Collections: | Research publications |
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