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Title: | Opportunistic feeding habits of two African freshwater clupeid fishes: DNA metabarcoding unravels spatial differences in diet and microbiome, and identifies new prey taxa | Authors: | Huyghe, Charlotte E. T. Aerts, Dorien N. Heindler, Franz M. KMENTOVA, Nikol Cirhuza, Deo Mushagalusa Hellemans, Bart Manda, Auguste Chocha VANHOVE, Maarten N'Sibula, Theophile Mulimbwa Volckaert, Filip A. M. Mulungula, Pascal Masilya De Keyzer, Els L. R. |
Issue Date: | 2023 | Publisher: | SPRINGER | Source: | HYDROBIOLOGIA, 850, p. 3777–3796 | Abstract: | The African Lake Tanganyika clupeids play an important role in the lake's ecosystem and have a high regional economic and nutritional value. Using DNA metabarcoding, we analysed the prey item composition and microbiome of these two clupeid species, Stolothrissa tanganicae and Limnothrissa miodon. We sequenced the mitochondrial COI region of the gut content for prey analysis and the 16S rRNA region of the hindgut content for microbiome analysis of 140 fish sampled at five locations across Lake Tanganyika. Our research confirmed previously reported prey items and discovered prey items that were not reported before, including the jellyfish Limnocnida tanganjicae. The hindgut of the fish harboured 15 bacterial phyla, with the most common being Firmicutes and Proteobacteria. The two clupeid species differed in diet, but not in microbiome. Further, the diet of S. tanganicae, but not its microbiome, varied on a spatial scale, whereas the microbiome, but not the diet, of L. miodon showed spatial variation. Our findings suggest that the Lake Tanganyika clupeids are opportunists, with a diet reflecting the local zooplankton community's composition. These results can serve as a useful reference for monitoring the health status of economically important fish stocks. | Notes: | Huyghe, CET (corresponding author), Katholieke Univ Leuven, Dept Biol, Lab Biodivers & Evolutionary Genom, Charles Deberiotstr 32, B-3000 Leuven, Belgium.; Huyghe, CET (corresponding author), Univ Basel, Zool Inst, Dept Environm Sci, Vesalgasse 1, CH-4051 Basel, Switzerland. charlotte.e.t.huyghe@gmail.com |
Keywords: | Biodiversity;Hindgut;Lake Tanganyika;Limnothrissa miodon;Sardine;Stomach content;Stolothrissa tanganicae | Document URI: | http://hdl.handle.net/1942/40779 | ISSN: | 0018-8158 | e-ISSN: | 1573-5117 | DOI: | 10.1007/s10750-023-05267-7 | ISI #: | 001034208900002 | Rights: | The Author(s), under exclusive licence to Springer Nature Switzerland AG 2023 | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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s10750-023-05267-7.pdf Restricted Access | Published version | 1.72 MB | Adobe PDF | View/Open Request a copy |
preprint 1.pdf | Peer-reviewed author version | 3.01 MB | Adobe PDF | View/Open |
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