Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/40782
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dc.contributor.authorMOYA GOMEZ, Amanda-
dc.contributor.authorFont, Lena Perez-
dc.contributor.authorBURLACU, Andreea-
dc.contributor.authorAGUIAR ALPIZAR, Yeranddy-
dc.contributor.authorCardonne, Miriam Maranon-
dc.contributor.authorBRONE, Bert-
dc.contributor.authorBRONCKAERS, Annelies-
dc.date.accessioned2023-08-29T07:35:09Z-
dc.date.available2023-08-29T07:35:09Z-
dc.date.issued2023-
dc.date.submitted2023-08-11T11:04:29Z-
dc.identifier.citationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (13) (Art N° 11117)-
dc.identifier.urihttp://hdl.handle.net/1942/40782-
dc.description.abstractExtremely low-frequency electromagnetic stimulation (ELF-EMS) was demonstrated to be significantly beneficial in rodent models of permanent stroke. The mechanism involved enhanced cerebrovascular perfusion and endothelial cell nitric oxide production. However, the possible effect on the neuroinflammatory response and its efficacy in reperfusion stroke models remains unclear. To evaluate ELF-EMS effectiveness and possible immunomodulatory response, we studied neurological outcome, behavior, neuronal survival, and glial reactivity in a rodent model of global transient stroke treated with 13.5 mT/60 Hz. Next, we studied microglial cells migration and, in organotypic hippocampal brain slices, we assessed neuronal survival and microglia reactivity. ELF-EMS improved the neurological score and behavior in the ischemia-reperfusion model. It also improved neuronal survival and decreased glia reactivity in the hippocampus, with microglia showing the first signs of treatment effect. In vitro ELF-EMS decreased (Lipopolysaccharide) LPS and ATP-induced microglia migration in both scratch and transwell assay. Additionally, in hippocampal brain slices, reduced microglial reactivity, improved neuronal survival, and modulation of inflammation-related markers was observed. Our study is the first to show that an EMF treatment has a direct impact on microglial migration. Furthermore, ELF-EMS has beneficial effects in an ischemia/reperfusion model, which indicates that this treatment has clinical potential as a new treatment against ischemic stroke.-
dc.description.sponsorshipThis research was funded by the Belgian Development Cooperation through VLIR-UOS (Flemish Interuniversity Council, University Cooperation for Development) in the context of the Institutional University Cooperation Program with Universidad de Oriente; the Institutional Research Founds of the Centro Nacional de Electromagnetismo Aplicado, Cuba; the BOF-BILA, doctoral grants in the framework of the bilateral cooperation, Hasselt University [BOF18BL17]; and the VLIR-UOS program Global Mind as well as a BOF NI grant number BOF18NI06 from Hasselt University. The authors would like to thank the help of Petra Bex, Evelyne Van Kerckhove and Leen Timmermans for its excellent technical assistant. We would also like to show gratitude to Jana Van Broeckhoven, Sarra Zaghbouni, Keerthana Ramanathan and Antonio Garcia Leyva for their help. We acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments and Sam Duwé (AOMC) for training and access to instrumentation. Figure 1 and Graphical abstract were created using BioRender.-
dc.language.isoen-
dc.publisherMDPI-
dc.rights2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).-
dc.subject.otherextremely low frequency electromagnetic stimulation-
dc.subject.otherischemic stroke-
dc.subject.otherischemia-
dc.subject.otherreperfusion injury-
dc.subject.othermicroglia migration-
dc.titleExtremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Improves Neurological Outcome and Reduces Microglial Reactivity in a Rodent Model of Global Transient Stroke-
dc.typeJournal Contribution-
dc.identifier.issue13-
dc.identifier.volume24-
local.format.pages20-
local.bibliographicCitation.jcatA1-
dc.description.notesBronckaers, A (corresponding author), Univ Oriente, Fac Ingn Informat Telecomunicac & Biomed, Biomed Engn Dept, Santiago De Cuba, Cuba.-
dc.description.notesamanda.moyagomez@uhasselt.be; bert.brone@uhasselt.be;-
dc.description.notesannelies.bronckaers@uhasselt.be-
local.publisher.placeST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr11117-
dc.identifier.doi10.3390/ijms241311117-
dc.identifier.pmid37446295-
dc.identifier.isi001031135300001-
dc.contributor.orcidBrone, Bert/0000-0002-4851-9480-
local.provider.typewosris-
local.description.affiliation[Moya-Gomez, Amanda; Burlacu, Andreea; Alpizar, Yeranddy A. A.; Brone, Bert] UHasselt, BIOMED, Agoralaan, B-3590 Diepenbeek, Belgium.-
local.description.affiliation[Moya-Gomez, Amanda; Cardonne, Miriam Maranon; Bronckaers, Annelies] Univ Oriente, Fac Ingn Informat Telecomunicac & Biomed, Biomed Engn Dept, Santiago De Cuba, Cuba.-
local.description.affiliation[Font, Lena Perez] Univ Oriente, Ctr Nacl Electromagnetismo Aplicado, Santiago De Cuba, Cuba.-
local.uhasselt.internationalyes-
item.fulltextWith Fulltext-
item.fullcitationMOYA GOMEZ, Amanda; Font, Lena Perez; BURLACU, Andreea; AGUIAR ALPIZAR, Yeranddy; Cardonne, Miriam Maranon; BRONE, Bert & BRONCKAERS, Annelies (2023) Extremely Low-Frequency Electromagnetic Stimulation (ELF-EMS) Improves Neurological Outcome and Reduces Microglial Reactivity in a Rodent Model of Global Transient Stroke. In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (13) (Art N° 11117).-
item.contributorMOYA GOMEZ, Amanda-
item.contributorFont, Lena Perez-
item.contributorBURLACU, Andreea-
item.contributorAGUIAR ALPIZAR, Yeranddy-
item.contributorCardonne, Miriam Maranon-
item.contributorBRONE, Bert-
item.contributorBRONCKAERS, Annelies-
item.accessRightsOpen Access-
crisitem.journal.issn1661-6596-
crisitem.journal.eissn1422-0067-
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