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http://hdl.handle.net/1942/42189
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DC Field | Value | Language |
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dc.contributor.author | Christensen, Kasper T. | - |
dc.contributor.author | Pierard, Florian | - |
dc.contributor.author | Bonsall, David | - |
dc.contributor.author | Bowden, Rory | - |
dc.contributor.author | Barnes, Eleanor | - |
dc.contributor.author | Florence, Eric | - |
dc.contributor.author | Ansari, M. Azim | - |
dc.contributor.author | Nguyen , Dung | - |
dc.contributor.author | de Cesare, Mariateresa | - |
dc.contributor.author | Nevens, Frederik | - |
dc.contributor.author | ROBAEYS, Geert | - |
dc.contributor.author | Schrooten, Yoeri | - |
dc.contributor.author | BUSSCHOTS, Dana | - |
dc.contributor.author | Simmonds, Peter | - |
dc.contributor.author | Vandamme , Anne-Mieke | - |
dc.contributor.author | Van Wijngaerden, Eric | - |
dc.contributor.author | Dierckx , Tim | - |
dc.contributor.author | Cuypers, Lize | - |
dc.contributor.author | Van Laethem, Kristel | - |
dc.date.accessioned | 2024-01-19T11:50:12Z | - |
dc.date.available | 2024-01-19T11:50:12Z | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2024-01-19T10:42:38Z | - |
dc.identifier.citation | Viruses-Basel, 15 (12) (Art N° 2391) | - |
dc.identifier.uri | http://hdl.handle.net/1942/42189 | - |
dc.description.abstract | The hepatitis C virus (HCV) epidemic in Western countries is primarily perpetuated by the sub-populations of men who have sex with men (MSM) and people who inject drugs (PWID). Understanding the dynamics of transmission in these communities is crucial for removing the remaining hurdles towards HCV elimination. We sequenced 269 annotated HCV plasma samples using probe enrichment and next-generation sequencing, obtaining 224 open reading frames of HCV (OR497849-OR498072). Maximum likelihood phylogenies were generated on the four most prevalent subtypes in this study (HCV1a, 1b, 3a, 4d) with a subsequent transmission cluster analysis. The highest rate of clustering was observed for HCV4d samples (13/17 (76.47%)). The second highest rate of clustering was observed in HCV1a samples (42/78 (53.85%)) with significant association with HIV-positive MSM. HCV1b and HCV3a had very low rates of clustering (2/83 (2.41%) and (0/29)). The spread of the prevalent subtype HCV1b appears to have been largely curtailed, and we demonstrate the onwards transmission of HCV1a and HCV4d in the HIV-positive MSM population across municipal borders. More systematic data collection and sequencing is needed to allow a better understanding of the HCV transmission among the community of PWID and overcome the remaining barriers for HCV elimination in Belgium. | - |
dc.description.sponsorship | This research and Kasper Thorhauge Christensen was funded by grants from the Fonds Wetenschappelijk Onderzoek Vlaanderen (FWO) (G069214, G0B2317N and 1S38819N). Lize Cuypers acknowledges FWO travel grant for a research visit at the University of Oxford (V431117N). Core funding to the Wellcome Centre for Human Genetics was provided by the Wellcome Trust (award 203141/Z/16/Z). The authors acknowledge the STOP-HCV consortium that was funded by a grant from the Medical Research Council, United Kingdom (MR/K01532X/1). | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.rights | 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | - |
dc.subject.other | hepatitis c | - |
dc.subject.other | human immunodeficiency virus | - |
dc.subject.other | phylogenetic analysis | - |
dc.subject.other | full-genome sequencing | - |
dc.subject.other | men who have sex with men | - |
dc.subject.other | people who inject drugs | - |
dc.subject.other | next-generation sequencing | - |
dc.title | Phylogenetic Analysis of Hepatitis C Virus Infections in a Large Belgian Cohort Using Next-Generation Sequencing of Full-Length Genomes | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 12 | - |
dc.identifier.volume | 15 | - |
local.format.pages | 15 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | Christensen, KT (corresponding author), Katholieke Univ Leuven, Rega Inst Med Res, Dept Microbiol Immunol & Transplantat, Lab Clin & Epidemiol Virol, B-3000 Leuven, Belgium. | - |
dc.description.notes | kasperthorhauge.christensen@kuleuven.be; florian.pierard@kuleuven.be; | - |
dc.description.notes | david.bonsall@bdi.ox.ac.uk; bowden.r@wehi.edu.au; | - |
dc.description.notes | ellie.barnes@ndm.ox.ac.uk; eric.florence@uza.be; | - |
dc.description.notes | azim.ansari@ndm.ox.ac.uk; dung.nguyen@ndm.ox.ac.uk; | - |
dc.description.notes | mariateresa.decesare@fht.org; frederik.nevens@uzleuven.be; | - |
dc.description.notes | geert.robaeys@uhasselt.be; yoeri.schrooten@uzleuven.be; | - |
dc.description.notes | danabusschots@hotmail.com; peter.simmonds@ndm.ox.ac.uk; | - |
dc.description.notes | annemie.vandamme@kuleuven.be; eric.vanwijngaerden@uzleuven.be; | - |
dc.description.notes | tim.dierckx@kuleuven.be; lize.cuypers@uzleuven.be; | - |
dc.description.notes | kristel.vanlaethem@uzleuven.be | - |
local.publisher.place | ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 2391 | - |
dc.identifier.doi | 10.3390/v15122391 | - |
dc.identifier.pmid | 38140632 | - |
dc.identifier.isi | 001130706100001 | - |
dc.contributor.orcid | Van Laethem, Kristel/0000-0001-6036-2271 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Christensen, Kasper T.; Pierard, Florian; Schrooten, Yoeri; Vandamme, Anne-Mieke; Dierckx, Tim; Cuypers, Lize; Van Laethem, Kristel] Katholieke Univ Leuven, Rega Inst Med Res, Dept Microbiol Immunol & Transplantat, Lab Clin & Epidemiol Virol, B-3000 Leuven, Belgium. | - |
local.description.affiliation | [Bonsall, David] Univ Oxford, Big Data Inst, Li Ka Shing Ctr Hlth Informat & Discovery, Nuffield Dept Med, Oxford OX3 7LF, England. | - |
local.description.affiliation | [Bonsall, David; Bowden, Rory; Nguyen, Dung; de Cesare, Mariateresa] Univ Oxford, Wellcome Ctr Human Genet, Roosevelt Dr, Oxford OX3 7BN, England. | - |
local.description.affiliation | [Barnes, Eleanor] Univ Oxford, Peter Medawar Bldg Pathogen Res, Oxford OX1 3SY, England. | - |
local.description.affiliation | [Barnes, Eleanor] Univ Oxford, Translat Gastroenterol Unit, Oxford OX3 9DU, England. | - |
local.description.affiliation | [Barnes, Eleanor] Univ Oxford, NIHR Oxford Biomed Res Ctr, Oxford OX3 9DU, England. | - |
local.description.affiliation | [Florence, Eric] Antwerp Univ Hosp, Dept Gen Internal Med Infect Dis & Trop Med, B-2650 Edegem, Belgium. | - |
local.description.affiliation | [Florence, Eric] Inst Trop Med, Dept Clin Sci, B-2000 Antwerp, Belgium. | - |
local.description.affiliation | [Ansari, M. Azim] Univ Oxford, Nuffield Dept Med, Oxford OX3 7BN, England. | - |
local.description.affiliation | [Nevens, Frederik; Robaeys, Geert] Univ Hosp Leuven, Dept Gastroenterol & Hepatol, B-3000 Leuven, Belgium. | - |
local.description.affiliation | [Robaeys, Geert; Busschots, Dana] UHasselt, Fac Med & Life Sci LCRC, B-3590 Diepenbeek, Belgium. | - |
local.description.affiliation | [Robaeys, Geert; Busschots, Dana] Ziekenhuis Oost Limburg, Dept Gastroenterol & Hepatol, B-3600 Genk, Belgium. | - |
local.description.affiliation | [Schrooten, Yoeri; Cuypers, Lize; Van Laethem, Kristel] Univ Hosp Leuven, Dept Lab Med, B-3000 Leuven, Belgium. | - |
local.description.affiliation | [Simmonds, Peter] Univ Oxford, Nuffield Dept Med, Henry Wellcome Bldg Mol Physiol, Old Rd Campus, Oxford OX3 7BN, England. | - |
local.description.affiliation | [Vandamme, Anne-Mieke] Univ Nova Lisboa, Inst Hyg & Trop Med, Global Hlth & Trop Med, Rua Junqueira 100, P-1349008 Lisbon, Portugal. | - |
local.description.affiliation | [Van Wijngaerden, Eric] Univ Hosp Leuven, Dept Gen Internal Med, B-3000 Leuven, Belgium. | - |
local.description.affiliation | [Cuypers, Lize] Katholieke Univ Leuven, Dept Microbiol Immunol & Transplantat, Lab Clin Microbiol, B-3000 Leuven, Belgium. | - |
local.uhasselt.international | yes | - |
item.fulltext | With Fulltext | - |
item.fullcitation | Christensen, Kasper T.; Pierard, Florian; Bonsall, David; Bowden, Rory; Barnes, Eleanor; Florence, Eric; Ansari, M. Azim; Nguyen , Dung; de Cesare, Mariateresa; Nevens, Frederik; ROBAEYS, Geert; Schrooten, Yoeri; BUSSCHOTS, Dana; Simmonds, Peter; Vandamme , Anne-Mieke; Van Wijngaerden, Eric; Dierckx , Tim; Cuypers, Lize & Van Laethem, Kristel (2023) Phylogenetic Analysis of Hepatitis C Virus Infections in a Large Belgian Cohort Using Next-Generation Sequencing of Full-Length Genomes. In: Viruses-Basel, 15 (12) (Art N° 2391). | - |
item.accessRights | Open Access | - |
item.contributor | Christensen, Kasper T. | - |
item.contributor | Pierard, Florian | - |
item.contributor | Bonsall, David | - |
item.contributor | Bowden, Rory | - |
item.contributor | Barnes, Eleanor | - |
item.contributor | Florence, Eric | - |
item.contributor | Ansari, M. Azim | - |
item.contributor | Nguyen , Dung | - |
item.contributor | de Cesare, Mariateresa | - |
item.contributor | Nevens, Frederik | - |
item.contributor | ROBAEYS, Geert | - |
item.contributor | Schrooten, Yoeri | - |
item.contributor | BUSSCHOTS, Dana | - |
item.contributor | Simmonds, Peter | - |
item.contributor | Vandamme , Anne-Mieke | - |
item.contributor | Van Wijngaerden, Eric | - |
item.contributor | Dierckx , Tim | - |
item.contributor | Cuypers, Lize | - |
item.contributor | Van Laethem, Kristel | - |
crisitem.journal.issn | 1999-4915 | - |
crisitem.journal.eissn | 1999-4915 | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Phylogenetic Analysis of Hepatitis C Virus.pdf | Published version | 28.58 MB | Adobe PDF | View/Open |
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