Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/42258
Title: | Cell-Interactive Gelatin-Based 19F MRI Tracers: An In Vitro Proof-of-Concept Study | Authors: | Kolouchova, Kristyna Groborz, Ondrej Herynek, Vit Petrov, Oleg V. Lang, Jan Dunlop, David Parmentier, Laurens Szabo, Anna Schaubroeck, David ADRIAENSENS, Peter Van Vlierberghe, Sandra |
Issue Date: | 2023 | Publisher: | AMER CHEMICAL SOC | Source: | CHEMISTRY OF MATERIALS, 36 (1) , p. 183 -196 | Abstract: | Cross-linked gelatin-based hydrogels are highly promising cell-interactive, biocompatible, and biodegradable materials serving tissue engineering. Moreover, gelatins with covalently bound methacrylamide (gel-MA) and 2-aminoethyl methacrylate moieties (gel-AEMA) can be cross-linked through ultraviolet (UV) irradiation, which allows light-based three-dimensional (3D)-printing of such hydrogels. Furthermore, the physicochemical and biological properties of these hydrogels can be broadly tuned by incorporating various comonomers into the polymer chains, which makes these hydrogels a widely applicable platform in tissue engineering and reconstructive surgery. However, monitoring the degradation rate of hydrogel-based implants in vivo is challenging, thereby prohibiting their broad clinical transition and further research. Therefore, herein, we describe the synthesis of 3D-printable gelatin-based hydrogels with N-(2,2-difluoroethyl)acrylamide (DFEA), detectable with the chemical shift of -123 ppm, which enables us to monitor these implants in vivo with F-19 magnetic resonance imaging (MRI) and assess their degradation kinetics. Next, we describe the physicochemical and biological properties of these hydrogels. Adding DFEA monomers into the reaction mixture accelerates their cross-linking kinetics. Moreover, increasing the DFEA content within the hydrogels increases their swelling ratio and F-19 MRI signal. All hydrogels were detectable at small quantities (<16 mg) using F-19 MRI. Moreover, our hydrogels supported the cell proliferation of adipose tissue-derived stem cells (ASCs) and had tunable biodegradation rates. Finally, we present a strategy for increasing the DFEA content without affecting the mechanical properties. Our results may be implemented in the future development of hydrogel implants, whose fate and biodegradation rate can be monitored via F-19 MRI. | Notes: | Kolouchova, K; Van Vlierberghe, S (corresponding author), Univ Ghent, Ctr Macromol Chem, Dept Organ & Macromol Chem, Polymer Chem & Biomat Grp, B-9000 Ghent, Belgium.; Van Vlierberghe, S (corresponding author), BIO INX BV, B-9052 Ghent, Belgium.; Van Vlierberghe, S (corresponding author), 4Tissue BV, B-9052 Ghent, Belgium. Kristyna.Kolouchova@UGent.be; Sandra.VanVlierberghe@UGent.be |
Document URI: | http://hdl.handle.net/1942/42258 | ISSN: | 0897-4756 | e-ISSN: | 1520-5002 | DOI: | 10.1021/acs.chemmater.3c01574 | ISI #: | 001139461500001 | Rights: | 2023 American Chemical Society | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
kolouchova-et-al-2023-cell-interactive-gelatin-based-19f-mri-tracers-an-in-vitro-proof-of-concept-study.pdf Restricted Access | Published version | 8.43 MB | Adobe PDF | View/Open Request a copy |
auteursversie.pdf Until 2024-05-17 | Peer-reviewed author version | 3.23 MB | Adobe PDF | View/Open Request a copy |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.