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http://hdl.handle.net/1942/42628
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DC Field | Value | Language |
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dc.contributor.advisor | Hooyberghs | - |
dc.contributor.author | STULENS, Yannick | - |
dc.contributor.author | VAN HOOF, Rebekka | - |
dc.contributor.author | MUELLER, Brittany | - |
dc.contributor.author | Hollanders, Karen | - |
dc.contributor.author | Jacobs, An | - |
dc.contributor.author | Kolpashchikov, Dmitry | - |
dc.contributor.author | Nelissen, Inge | - |
dc.contributor.author | WAGNER, Patrick | - |
dc.contributor.author | HOOYBERGHS, Jef | - |
dc.date.accessioned | 2024-03-12T15:00:14Z | - |
dc.date.available | 2024-03-12T15:00:14Z | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2024-03-11T13:23:44Z | - |
dc.identifier.citation | - | |
dc.identifier.uri | http://hdl.handle.net/1942/42628 | - |
dc.description.abstract | Mutation detection is of great importance in many biological processes and diagnostics. Single-nucleotide variations are common, where a single base-pair is substituted during replication of wild-type DNA. Duplex formation via hybridisation provides an important detection technique where one of the strands is designed to match the mutant sequence and probe its presence. The probe is, however, subject to cross-hybridisation of wild-type DNA, lowering detection sensitivity in case of low-abundant mutants in a majority wild-type background. We use thermodynamics-based probe design and Langmuir theory to increase sensitivity and quantify the amount of mutant in a sample. | - |
dc.language.iso | en | - |
dc.title | Use of DNA thermodynamics for low-abundance mutation detection by DNA hybridization | - |
dc.type | Conference Material | - |
local.bibliographicCitation.jcat | C2 | - |
dc.relation.references | [1] R. Van Hoof, M. Szymonik, S.K. Nomidis, K. Hollanders, A. Jacobs, I. Nelissen, P. Wagner and J. Hooyberghs, ”Depletion of wild-type target enhances the hybridization-based sensitivity of low- abundant mutation detection by reference capture probes”, Sensors and Actuators B: Chemical, vol. 368, 2022. [2] S.K.Nomidis,M.Szymonik,T.Venken,E.CarlonandJ.Hooyberghs,”EnhancingthePerformance of DNA Surface-Hybridization Biosensors through Target Depletion”, Langmuir, vol. 35, 2019. | - |
local.type.refereed | Non-Refereed | - |
local.type.specified | Conference Presentation | - |
local.provider.type | - | |
local.uhasselt.international | no | - |
item.fulltext | With Fulltext | - |
item.fullcitation | STULENS, Yannick; VAN HOOF, Rebekka; MUELLER, Brittany; Hollanders, Karen; Jacobs, An; Kolpashchikov, Dmitry; Nelissen, Inge; WAGNER, Patrick & HOOYBERGHS, Jef (2023) Use of DNA thermodynamics for low-abundance mutation detection by DNA hybridization. | - |
item.accessRights | Open Access | - |
item.contributor | STULENS, Yannick | - |
item.contributor | VAN HOOF, Rebekka | - |
item.contributor | MUELLER, Brittany | - |
item.contributor | Hollanders, Karen | - |
item.contributor | Jacobs, An | - |
item.contributor | Kolpashchikov, Dmitry | - |
item.contributor | Nelissen, Inge | - |
item.contributor | WAGNER, Patrick | - |
item.contributor | HOOYBERGHS, Jef | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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YannickStulens_BPS_Abstract.pdf | Conference material | 525.96 kB | Adobe PDF | View/Open |
YannickStulens_BPS2023.pdf | Conference material | 8.45 MB | Adobe PDF | View/Open |
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