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Title: | Generating human skeletal myoblast spheroids for vascular myogenic tissue engineering | Authors: | Minne, Mendy Terrie, Lisanne Wüst, Rebecca HASEVOETS, Steffie Vanden Kerchove, Kato Nimako, Kakra LAMBRICHTS, Ivo Thorrez, Lieven Declercq, Heidi |
Issue Date: | 2024 | Publisher: | IOP Publishing Ltd | Source: | Biofabrication, 16 (2) (Art N° 025035) | Abstract: | Engineered myogenic microtissues derived from human skeletal myoblasts offer unique opportunities for varying skeletal muscle tissue engineering applications, such as in vitro drug-testing and disease modelling. However, more complex models require the incorporation of vascular structures, which remains to be challenging. In this study, myogenic spheroids were generated using a high-throughput, non-adhesive micropatterned surface. Since monoculture spheroids containing human skeletal myoblasts were unable to remain their integrity, co-culture spheroids combining human skeletal myoblasts and human adipose-derived stem cells were created. When using the optimal ratio, uniform and viable spheroids with enhanced myogenic properties were achieved. Applying a pre-vascularization strategy, through addition of endothelial cells, resulted in the formation of spheroids containing capillary-like networks, lumina and collagen in the extracellular matrix, whilst retaining myogenicity. Moreover, sprouting of endothelial cells from the spheroids when encapsulated in fibrin was allowed. The possibility of spheroids, from different maturation stages, to assemble into a more large construct was proven by doublet fusion experiments. The relevance of using 3D microtissues with tissue-specific microarchitecture and increased complexity, together with the high-throughput generation approach, makes the generated spheroids a suitable tool for in vitro drug-testing and human disease modelling. | Notes: | Minne, M (corresponding author), Dept Dev & Regenerat, Tissue Engn Lab, KU Leuven, Campus KULAK, Kortrijk, Belgium. | Keywords: | spheroids;co-culture;myogenic tissue;vascularization;3D culture | Document URI: | http://hdl.handle.net/1942/42707 | ISSN: | 1758-5082 | e-ISSN: | 1758-5090 | DOI: | 10.1088/1758-5090/ad2fd5 | ISI #: | 001188550000001 | Rights: | 2024TheAuthor(s). Published by IOPPublishing Ltd Original content from this work may be used under the terms of the Creative Commons Attribution 4.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI. | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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Minne_2024_Biofabrication_16_025035.pdf | Published version | 7.14 MB | Adobe PDF | View/Open |
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