Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/42785
Title: | The Impact of Sample Storage on Blood Methylation: Towards Assessing Myelin Gene Methylation as a Biomarker for Progressive Multiple Sclerosis | Authors: | TIANE, Assia SOMERS, Veerle HELLINGS, Niels van den Hove, DLA VANMIERLO, Tim |
Issue Date: | 2024 | Publisher: | MDPI | Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 25 (6) (Art N° 3468) | Abstract: | One of the major challenges in multiple sclerosis (MS) is to accurately monitor and quantify disability over time. Thus, there is a pressing need to identify new biomarkers for disease progression. Peripheral blood DNA methylation has been demonstrated to be an easily accessible and quantifiable marker in many neurodegenerative diseases. In this study, we aimed to investigate whether methylation patterns that were previously determined in chronic inactive white matter lesions of patients with progressive MS are also reflected in the blood, and whether the latter can serve as a biomarker for disease progression in MS. While our initial analysis revealed differences in the blood methylation state of important myelin-related genes between patients with progressive MS and controls, these findings could not be validated in other independent patient cohorts. Subsequent investigation suggests that sample storage can selectively influence DNA methylation patterns, potentially hindering accurate epigenetic analysis. Therefore, sample storage time should be taken into consideration during the initial sample selection stage in biomarker studies. | Notes: | Vanmierlo, T (corresponding author), Hasselt Univ, Biomed Res Inst, Fac Med & Life Sci, Dept Neurosci, B-3500 Hasselt, Belgium.; Vanmierlo, T (corresponding author), Maastricht Univ, Sch Mental Hlth & Neurosci, Dept Psychiat & Neuropsychol, NL-6229 Maastricht, Netherlands.; Vanmierlo, T (corresponding author), Univ MS Ctr UMSC, B-3500 Hasselt, Belgium. veerle.somers@uhasselt.be; niels.hellings@uhasselt.be; d.vandenhove@maastrichtuniversity.nl; tim.vanmierlo@uhasselt.be |
Keywords: | biomarker;DNA methylation;(re)myelination;multiple sclerosis;epigenetics | Document URI: | http://hdl.handle.net/1942/42785 | ISSN: | 1661-6596 | e-ISSN: | 1422-0067 | DOI: | 10.3390/ijms25063468 | ISI #: | 001192920200001 | Rights: | 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
ijms-25-03468.pdf | Published version | 621.36 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.