Please use this identifier to cite or link to this item:
http://hdl.handle.net/1942/42812
Title: | Development of a Bioaerosol Sampling Method for Airborne Pathogen Detection with Focus on SARS-CoV-2 | Authors: | Paralovo, Sarah L. VANDEN DRIESSCHE , Koen Cartuyvels, Reinoud Lazarov, Borislav Vlieghe, Erika VANSTRAELEN, Laura Smets, Rita Spruyt, Maarten Kreps, Sabine Hufkens, Nady Stranger, Marianne |
Editors: | Wang, Faming | Issue Date: | 2024 | Publisher: | WILEY-HINDAWI | Source: | INDOOR AIR, 2024 (Art N° 6638511) | Abstract: | As worldwide evidence shows that the predominant transmission route of SARS-CoV-2 and other respiratory pathogens is airborne, the need for suitable methods for the sampling of bioparticles directly from the air is more urgent than ever. The present paper describes the development of a method for the collection of biological aerosols, using a preexisting cyclonic impinger, the Coriolis mu, combined with a lysis buffer and subsequent qPCR analysis of the generated samples in lab. Four phases of method development are described: exploratory, validation, blank tests, and application. The application phase consisted of a field experiment in which the method was simultaneously applied at two daycare facilities. The method achieved a good level of accuracy and reliability in detecting different types of infectious agents in the air, with a global uncertainty of 19.6%. Furthermore, our method allows the simultaneous detection of 26 different respiratory pathogens in air samples, it is relatively simple, and the equipment is easy to use. Additionally, the time to collect a representative sample is short compared to other methods. The method does not cause significant disturbance to those present in the sampled rooms, and it is safe for operators and flexible, meaning it can be used in virtually any environment regardless of use, size, or occupancy. Further research is being developed to allow quantitative analysis of the collected samples and to test the methods' ability to assess the viability of the microorganisms collected in the sample. | Notes: | Paralovo, SL (corresponding author), Flemish Inst Technol Res VITO, Indoor Air Qual Team, Mol, Belgium. sarah.limaparalovo@vito.be; koen.vandendriessche@uza.be; reinoud.cartuyvels@jessazh.be; borislav.lazarov@vito.be; erika.vlieghe@uza.be; laura.vanstraelen@jessazh.be; rita.smets@jessazh.be; maarten.spruyt@vito.be; sabine.kreps@vito.be; nady.hufkens@vito.be; marianne.stranger@vito.be |
Document URI: | http://hdl.handle.net/1942/42812 | ISSN: | 0905-6947 | e-ISSN: | 1600-0668 | DOI: | 10.1155/2024/6638511 | ISI #: | 001189708900001 | Rights: | 2024 Sarah L. Paralovo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.