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http://hdl.handle.net/1942/43149
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DC Field | Value | Language |
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dc.contributor.author | Deckers, C | - |
dc.contributor.author | Bélik, F | - |
dc.contributor.author | Denis, O | - |
dc.contributor.author | Montesinos, I | - |
dc.contributor.author | Bogaerts , P | - |
dc.contributor.author | Boelens, J | - |
dc.contributor.author | Brassinne, L | - |
dc.contributor.author | Descy, J | - |
dc.contributor.author | Desmet, S | - |
dc.contributor.author | Gils, S | - |
dc.contributor.author | Lissoir, B | - |
dc.contributor.author | Matheeussen, V | - |
dc.contributor.author | MAGERMAN, Koen | - |
dc.contributor.author | Meex, C | - |
dc.contributor.author | Villalobos, HR | - |
dc.contributor.author | Van den Abeele, AM | - |
dc.contributor.author | Vernelen, K | - |
dc.contributor.author | Ceyssens, PJ | - |
dc.contributor.author | Huang, TD | - |
dc.date.accessioned | 2024-06-14T08:11:26Z | - |
dc.date.available | 2024-06-14T08:11:26Z | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2024-06-14T08:07:36Z | - |
dc.identifier.citation | EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 42 (12) , p. 1477 -1483 | - |
dc.identifier.uri | http://hdl.handle.net/1942/43149 | - |
dc.description.abstract | Accurate susceptibility result of temocillin (TMO) is important for treating infections caused by multidrug-resistant Enterobacterales. This multicenter study aimed to investigate the performance of routine temocillin testing assays against Enterobacterales challenging strains. Forty-seven selected clinical isolates were blindly analyzed by 12 Belgian laboratories using VITEK® 2 (n = 5) and BD Phoenix™ (n = 3) automated systems, ETEST® gradient strip (n = 3), and disk (3 brands) diffusion method (DD; n = 6) for temocillin susceptibility using standardized methodology. Results were interpreted using EUCAST 2023 criteria and compared to the broth microdilution (BMD; Sensititre™ panel) method used as gold standard. Methods' reproducibility was assessed by testing 3 reference strains in triplicate. A total of 702 organism-drug results were obtained against 33 TMO-susceptible and 14 TMO-resistant isolates. Excluding Proteae species (P. mirabilis and M. morganii), the essential agreement rates were excellent (91.5-100%) for all MIC-based methods. The highest category agreement was achieved by ETEST® (97.5%) followed by VITEK® 2 (93.2%), disk diffusion (91.6%), and BD Phoenix™ (88.5%). BD Phoenix™ and paper disk diffusion overcalled resistance (11.5% and 6.8% of major discrepancies, respectively), while ROSCO tablets diffusion and VITEK® 2 generated higher very major discrepancies (7.1% and 4.2% respectively). Inter-assay reproducibility was unsatisfactory using recommended E. coli ATCC 25922 strain but was excellent with E. coli ATCC 35218 and K. pneumoniae ATCC 700603 strains. This interlaboratory study suggests that routine testing methods provide accurate and reproducible TMO categorization results except for Proteae species. | - |
dc.description.sponsorship | Funding The study was funded by a specifc budget allocated by the National Antibiogram Committee through Federal Public Service. The Belgian National Reference Center is supported in part by the Belgian Ministry of Social Afairs through a fund within the national health insurance system (INAMI-RIZIV). Acknowledgements We thank the staf of the laboratories who participated this study and the members of the Belgian National Antimicrobial susceptibility testing Committee for their scientifc and logistic support: Jerina Boelens, Laetitia Brassinne, Lucy Catteau [Sciensano], Pieter-Jan Ceyssens, Julie Descy, Stefanie Desmet, Sarah Gils, Katrien Latour [Sciensano], Bénédicte Lissoir, Koen Magerman, Veerle Matheeussen, Cécile Meex, Hector Rodriguez-Villalobos, Sarah Vandamme [Universitaire Ziekenhuis Antwerpen], Anne-Marie Van den Abeele, Aline Vilain [Sciensano], Kris Vernelen, Ingrid Wybo [Universitaire Ziekenhuis Brussels], Harun Yaras [Belgian Antibiotic Policy Coordination Commission], Nicolas Yin [Laboratoire Hospitalier Universitaire de Bruxelles]. | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.rights | The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 | - |
dc.subject.other | Temocillin | - |
dc.subject.other | Antimicrobial susceptibility testing | - |
dc.subject.other | Enterobacterales | - |
dc.subject.other | EUCAST Breakpoints | - |
dc.title | Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 1483 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 1477 | - |
dc.identifier.volume | 42 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | ONE NEW YORK PLAZA, SUITE 4600 , NEW YORK, NY 10004, UNITED STATES | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1007/s10096-023-04681-y | - |
dc.identifier.pmid | 37870713 | - |
dc.identifier.isi | 001095817200004 | - |
local.provider.type | Web of Science | - |
local.uhasselt.international | no | - |
item.fullcitation | Deckers, C; Bélik, F; Denis, O; Montesinos, I; Bogaerts , P; Boelens, J; Brassinne, L; Descy, J; Desmet, S; Gils, S; Lissoir, B; Matheeussen, V; MAGERMAN, Koen; Meex, C; Villalobos, HR; Van den Abeele, AM; Vernelen, K; Ceyssens, PJ & Huang, TD (2023) Multicenter interlaboratory study of routine systems for the susceptibility testing of temocillin using a challenge panel of multidrug-resistant strains. In: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 42 (12) , p. 1477 -1483. | - |
item.accessRights | Restricted Access | - |
item.fulltext | With Fulltext | - |
item.contributor | Deckers, C | - |
item.contributor | Bélik, F | - |
item.contributor | Denis, O | - |
item.contributor | Montesinos, I | - |
item.contributor | Bogaerts , P | - |
item.contributor | Boelens, J | - |
item.contributor | Brassinne, L | - |
item.contributor | Descy, J | - |
item.contributor | Desmet, S | - |
item.contributor | Gils, S | - |
item.contributor | Lissoir, B | - |
item.contributor | Matheeussen, V | - |
item.contributor | MAGERMAN, Koen | - |
item.contributor | Meex, C | - |
item.contributor | Villalobos, HR | - |
item.contributor | Van den Abeele, AM | - |
item.contributor | Vernelen, K | - |
item.contributor | Ceyssens, PJ | - |
item.contributor | Huang, TD | - |
crisitem.journal.issn | 0934-9723 | - |
crisitem.journal.eissn | 1435-4373 | - |
Appears in Collections: | Research publications |
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