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http://hdl.handle.net/1942/43189
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DC Field | Value | Language |
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dc.contributor.author | Collins, A | - |
dc.contributor.author | Moller, P | - |
dc.contributor.author | Gajski, G | - |
dc.contributor.author | Vodenková, S | - |
dc.contributor.author | Abdulwahed, A | - |
dc.contributor.author | Anderson, D | - |
dc.contributor.author | Bankoglu, EE | - |
dc.contributor.author | Bonassi, S | - |
dc.contributor.author | Boutet-Robinet, E | - |
dc.contributor.author | Brunborg, G | - |
dc.contributor.author | Chao, C | - |
dc.contributor.author | Cooke, MS | - |
dc.contributor.author | Costa, C | - |
dc.contributor.author | Costa, S | - |
dc.contributor.author | Dhawan, A | - |
dc.contributor.author | de Lapuente, J | - |
dc.contributor.author | Del Bo, C | - |
dc.contributor.author | Dubus, J | - |
dc.contributor.author | Dusinska, M | - |
dc.contributor.author | Duthie, SJ | - |
dc.contributor.author | El Yamani, N | - |
dc.contributor.author | Engelward, B | - |
dc.contributor.author | Gaivao, I | - |
dc.contributor.author | Giovannelli, L | - |
dc.contributor.author | Godschalk , R | - |
dc.contributor.author | Guilherme, S | - |
dc.contributor.author | Gutzkow, KB | - |
dc.contributor.author | Habas, K | - |
dc.contributor.author | Hernández, A | - |
dc.contributor.author | Herrero, O | - |
dc.contributor.author | Isidori, M | - |
dc.contributor.author | Jha, AN | - |
dc.contributor.author | Knasmüller, S | - |
dc.contributor.author | Kooter, IM | - |
dc.contributor.author | Koppen, G | - |
dc.contributor.author | Kruszewski, M | - |
dc.contributor.author | Ladeira, C | - |
dc.contributor.author | Laffon, B | - |
dc.contributor.author | Larramendy, M | - |
dc.contributor.author | Le Hegarat, L | - |
dc.contributor.author | Lewies, A | - |
dc.contributor.author | Lewinska, A | - |
dc.contributor.author | Liwszyc, GE | - |
dc.contributor.author | de Cerain, AL | - |
dc.contributor.author | Manjanatha, M | - |
dc.contributor.author | Marcos, R | - |
dc.contributor.author | Milic, M | - |
dc.contributor.author | de Andrade, VM | - |
dc.contributor.author | Moretti , M | - |
dc.contributor.author | Muruzabal, D | - |
dc.contributor.author | Novak, M | - |
dc.contributor.author | Oliveira, R | - |
dc.contributor.author | Olsen, AK | - |
dc.contributor.author | Owiti, N | - |
dc.contributor.author | Pacheco, M | - |
dc.contributor.author | Pandey, AK | - |
dc.contributor.author | Pfuhler, S | - |
dc.contributor.author | Pourrut, B | - |
dc.contributor.author | Reisinger, K | - |
dc.contributor.author | Rojas, E | - |
dc.contributor.author | Rundén-Pran, E | - |
dc.contributor.author | Sanz-Serrano, J | - |
dc.contributor.author | Shaposhnikov, S | - |
dc.contributor.author | Sipinen, V | - |
dc.contributor.author | Stopper, H | - |
dc.contributor.author | Smeets, K | - |
dc.contributor.author | Teixeira, JP | - |
dc.contributor.author | Valdiglesias, V | - |
dc.contributor.author | Valverde, M | - |
dc.contributor.author | van Acker, F | - |
dc.contributor.author | van Schooten, FJ | - |
dc.contributor.author | Vasquez, M | - |
dc.contributor.author | Wentzel, JF | - |
dc.contributor.author | Wnuk, M | - |
dc.contributor.author | Wouters, A | - |
dc.contributor.author | Zegura, B | - |
dc.contributor.author | Zikmund, T | - |
dc.contributor.author | Langie, SAS | - |
dc.contributor.author | Azqueta, A | - |
dc.date.accessioned | 2024-06-18T08:04:27Z | - |
dc.date.available | 2024-06-18T08:04:27Z | - |
dc.date.issued | 2023 | - |
dc.date.submitted | 2024-06-18T07:54:22Z | - |
dc.identifier.citation | NATURE PROTOCOLS, 18 (3) , p. 929 -989 | - |
dc.identifier.issn | 1754-2189 | - |
dc.identifier.issn | 1750-2799 | - |
dc.identifier.uri | http://hdl.handle.net/1942/43189 | - |
dc.description.abstract | The comet assay is a versatile method to detect nuclear DNA damage in individual eukaryotic cells, from yeast to human. The types of damage detected encompass DNA strand breaks and alkali-labile sites (e.g., apurinic/apyrimidinic sites), alkylated and oxidized nucleobases, DNA-DNA crosslinks, UV-induced cyclobutane pyrimidine dimers and some chemically induced DNA adducts. Depending on the specimen type, there are important modifications to the comet assay protocol to avoid the formation of additional DNA damage during the processing of samples and to ensure sufficient sensitivity to detect differences in damage levels between sample groups. Various applications of the comet assay have been validated by research groups in academia, industry and regulatory agencies, and its strengths are highlighted by the adoption of the comet assay as an in vivo test for genotoxicity in animal organs by the Organisation for Economic Co-operation and Development. The present document includes a series of consensus protocols that describe the application of the comet assay to a wide variety of cell types, species and types of DNA damage, thereby demonstrating its versatility. | - |
dc.description.sponsorship | We thank the hCOMET project (COST Action, CA 15132) for support. A. Azqueta thanks the Ministry of Science and Innovation (AGL2015-70640-R and PID2020-115348RB-I00) of the Spanish Government. S.G. thanks the national funds (OE), through FCT— Fundação para a Ciência e a Tecnologia (IP, in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July) for personal support. V.M.d.A. thanks the National Council of Technological and Scientific Development (CNPq—304203/2018-1) for personal support. D.M. thanks the program ‘Ayudas para la formación de profesorado universitario (FPU)’ of the Spanish Government for the predoctoral grant received. N.O. thanks the NIEHS Superfund Research Program ES ES027707 for personal support. J.S.-S. thanks the Government of Navarra for the predoctoral grant received. V.V. thanks the Ministerio de Educación, Cultura y Deporte (‘Beatriz Galindo’ program, BEAGAL18/00142) of the Spanish Government for personal support. M.S.C. acknowledges personal support from the National Institute of Environmental Health Sciences of the National Institutes of Health under award number: 1R41ES030274-01. This paper reflects the views of the authors and does not necessarily reflect those of the US Food and Drug Administration or the National Institutes of Health. | - |
dc.language.iso | en | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.subject.other | Animals | - |
dc.subject.other | Humans | - |
dc.subject.other | Comet Assay | - |
dc.subject.other | Eukaryotic Cells | - |
dc.subject.other | DNA | - |
dc.subject.other | DNA Damage | - |
dc.subject.other | Pyrimidine Dimers | - |
dc.title | Measuring DNA modifications with the comet assay: a compendium of protocols | - |
dc.type | Journal Contribution | - |
dc.identifier.epage | 989 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 929 | - |
dc.identifier.volume | 18 | - |
local.bibliographicCitation.jcat | A1 | - |
local.publisher.place | HEIDELBERGER PLATZ 3, BERLIN 14197, GERMANY | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
dc.identifier.doi | 10.1038/s41596-022-00754-y | - |
dc.identifier.pmid | 36707722 | - |
dc.identifier.isi | WOS:000921193000001 | - |
dc.identifier.eissn | 1750-2799 | - |
local.provider.type | Web of Science | - |
local.uhasselt.international | yes | - |
item.fullcitation | Collins, A; Moller, P; Gajski, G; Vodenková, S; Abdulwahed, A; Anderson, D; Bankoglu, EE; Bonassi, S; Boutet-Robinet, E; Brunborg, G; Chao, C; Cooke, MS; Costa, C; Costa, S; Dhawan, A; de Lapuente, J; Del Bo, C; Dubus, J; Dusinska, M; Duthie, SJ; El Yamani, N; Engelward, B; Gaivao, I; Giovannelli, L; Godschalk , R; Guilherme, S; Gutzkow, KB; Habas, K; Hernández, A; Herrero, O; Isidori, M; Jha, AN; Knasmüller, S; Kooter, IM; Koppen, G; Kruszewski, M; Ladeira, C; Laffon, B; Larramendy, M; Le Hegarat, L; Lewies, A; Lewinska, A; Liwszyc, GE; de Cerain, AL; Manjanatha, M; Marcos, R; Milic, M; de Andrade, VM; Moretti , M; Muruzabal, D; Novak, M; Oliveira, R; Olsen, AK; Owiti, N; Pacheco, M; Pandey, AK; Pfuhler, S; Pourrut, B; Reisinger, K; Rojas, E; Rundén-Pran, E; Sanz-Serrano, J; Shaposhnikov, S; Sipinen, V; Stopper, H; Smeets, K; Teixeira, JP; Valdiglesias, V; Valverde, M; van Acker, F; van Schooten, FJ; Vasquez, M; Wentzel, JF; Wnuk, M; Wouters, A; Zegura, B; Zikmund, T; Langie, SAS & Azqueta, A (2023) Measuring DNA modifications with the comet assay: a compendium of protocols. In: NATURE PROTOCOLS, 18 (3) , p. 929 -989. | - |
item.fulltext | With Fulltext | - |
item.contributor | Collins, A | - |
item.contributor | Moller, P | - |
item.contributor | Gajski, G | - |
item.contributor | Vodenková, S | - |
item.contributor | Abdulwahed, A | - |
item.contributor | Anderson, D | - |
item.contributor | Bankoglu, EE | - |
item.contributor | Bonassi, S | - |
item.contributor | Boutet-Robinet, E | - |
item.contributor | Brunborg, G | - |
item.contributor | Chao, C | - |
item.contributor | Cooke, MS | - |
item.contributor | Costa, C | - |
item.contributor | Costa, S | - |
item.contributor | Dhawan, A | - |
item.contributor | de Lapuente, J | - |
item.contributor | Del Bo, C | - |
item.contributor | Dubus, J | - |
item.contributor | Dusinska, M | - |
item.contributor | Duthie, SJ | - |
item.contributor | El Yamani, N | - |
item.contributor | Engelward, B | - |
item.contributor | Gaivao, I | - |
item.contributor | Giovannelli, L | - |
item.contributor | Godschalk , R | - |
item.contributor | Guilherme, S | - |
item.contributor | Gutzkow, KB | - |
item.contributor | Habas, K | - |
item.contributor | Hernández, A | - |
item.contributor | Herrero, O | - |
item.contributor | Isidori, M | - |
item.contributor | Jha, AN | - |
item.contributor | Knasmüller, S | - |
item.contributor | Kooter, IM | - |
item.contributor | Koppen, G | - |
item.contributor | Kruszewski, M | - |
item.contributor | Ladeira, C | - |
item.contributor | Laffon, B | - |
item.contributor | Larramendy, M | - |
item.contributor | Le Hegarat, L | - |
item.contributor | Lewies, A | - |
item.contributor | Lewinska, A | - |
item.contributor | Liwszyc, GE | - |
item.contributor | de Cerain, AL | - |
item.contributor | Manjanatha, M | - |
item.contributor | Marcos, R | - |
item.contributor | Milic, M | - |
item.contributor | de Andrade, VM | - |
item.contributor | Moretti , M | - |
item.contributor | Muruzabal, D | - |
item.contributor | Novak, M | - |
item.contributor | Oliveira, R | - |
item.contributor | Olsen, AK | - |
item.contributor | Owiti, N | - |
item.contributor | Pacheco, M | - |
item.contributor | Pandey, AK | - |
item.contributor | Pfuhler, S | - |
item.contributor | Pourrut, B | - |
item.contributor | Reisinger, K | - |
item.contributor | Rojas, E | - |
item.contributor | Rundén-Pran, E | - |
item.contributor | Sanz-Serrano, J | - |
item.contributor | Shaposhnikov, S | - |
item.contributor | Sipinen, V | - |
item.contributor | Stopper, H | - |
item.contributor | Smeets, K | - |
item.contributor | Teixeira, JP | - |
item.contributor | Valdiglesias, V | - |
item.contributor | Valverde, M | - |
item.contributor | van Acker, F | - |
item.contributor | van Schooten, FJ | - |
item.contributor | Vasquez, M | - |
item.contributor | Wentzel, JF | - |
item.contributor | Wnuk, M | - |
item.contributor | Wouters, A | - |
item.contributor | Zegura, B | - |
item.contributor | Zikmund, T | - |
item.contributor | Langie, SAS | - |
item.contributor | Azqueta, A | - |
item.accessRights | Restricted Access | - |
Appears in Collections: | Research publications |
Files in This Item:
File | Description | Size | Format | |
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Measuring DNA modifications with the comet assay_ a compendium of protocols.pdf Restricted Access | Published version | 3.99 MB | Adobe PDF | View/Open Request a copy |
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