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http://hdl.handle.net/1942/43569
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DC Field | Value | Language |
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dc.contributor.author | Salz, Renee | - |
dc.contributor.author | Vorsteveld, Emil E. | - |
dc.contributor.author | van der Made, Caspar I. | - |
dc.contributor.author | Kersten, Simone | - |
dc.contributor.author | Stemerdink, Merel | - |
dc.contributor.author | Riepe, Tabea, V | - |
dc.contributor.author | Hsieh, Tsung-han | - |
dc.contributor.author | Mhlanga, Musa | - |
dc.contributor.author | Netea, Mihai G. | - |
dc.contributor.author | VOLDERS, Pieter-Jan | - |
dc.contributor.author | Hoischen, Alexander | - |
dc.contributor.author | 't Hoen, Peter A. C. | - |
dc.date.accessioned | 2024-08-19T09:13:31Z | - |
dc.date.available | 2024-08-19T09:13:31Z | - |
dc.date.issued | 2024 | - |
dc.date.submitted | 2024-08-19T08:31:13Z | - |
dc.identifier.citation | iScience (Cambridge), 27 (8) (Art N° 110471) | - |
dc.identifier.uri | http://hdl.handle.net/1942/43569 | - |
dc.description.abstract | We performed long-read transcriptome and proteome profiling of pathogen-stimulated peripheral blood mononuclear cells (PBMCs) from healthy donors to discover new transcript and protein isoforms expressed during immune responses to diverse pathogens. Long-read transcriptome profiling reveals novel sequences and isoform switching induced upon pathogen stimulation, including transcripts that are difficult to detect using traditional short-read sequencing. Widespread loss of intron retention occurs as a common result of all pathogen stimulations. We highlight novel transcripts of NFKB1 and CASP1 that may indicate novel immunological mechanisms. RNA expression differences did not result in differences in the amounts of secreted proteins. Clustering analysis of secreted proteins revealed a correlation between chemokine (receptor) expression on the RNA and protein levels in C. albicans- and poly(I:C)-stimulated PBMCs. Isoform aware long-read sequencing of pathogen-stimulated immune cells highlights the potential of these methods to identify novel transcripts, revealing a more complex transcriptome landscape than previously appreciated. | - |
dc.description.sponsorship | This work was partly supported by the Netherlands X-omics initiative, funded by the Netherlands Organisation for Scientific Research, project 184.034.019. MGN was supported by an ERC Advanced Grant (#833247) and a Spinoza grant of the Netherlands Organisation for Scientific Research. A.H. was supported by the Solve-RD project, which has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no. 779257. We thank all colleagues from the Radboud Genomics Technology Center and the VIB Proteomics Core for technical support. | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.rights | 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | - |
dc.subject.other | Immunology | - |
dc.subject.other | Proteomics | - |
dc.subject.other | Transcriptomics | - |
dc.title | Multi-omic profiling of pathogen-stimulated primary immune cells | - |
dc.type | Journal Contribution | - |
dc.identifier.issue | 8 | - |
dc.identifier.volume | 27 | - |
local.format.pages | 21 | - |
local.bibliographicCitation.jcat | A1 | - |
dc.description.notes | 't Hoen, PAC (corresponding author), Radboud Univ Nijmegen, Med Ctr, Dept Med BioSci, NL-6525 GA Nijmegen, Netherlands.; Hoischen, A; 't Hoen, PAC (corresponding author), Radboud Univ Nijmegen, RadboudUMC Res Inst Med Innovat, Med Ctr, NL-6525 GA Nijmegen, Netherlands.; Hoischen, A (corresponding author), Radboud Univ Nijmegen, Med Ctr, Dept Human Genet, NL-6525 GA Nijmegen, Netherlands.; Hoischen, A (corresponding author), Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 GA Nijmegen, Netherlands.; Hoischen, A (corresponding author), Radboud Univ Nijmegen, Radboud Ctr Infect Dis RCI, Med Ctr, NL-6525 GA Nijmegen, Netherlands. | - |
dc.description.notes | alexander.hoischen@radboudumc.nl; peter-bram.thoen@radboudumc.nl | - |
local.publisher.place | 50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA | - |
local.type.refereed | Refereed | - |
local.type.specified | Article | - |
local.bibliographicCitation.artnr | 110471 | - |
local.type.programme | H2020 | - |
local.relation.h2020 | 779257 | - |
dc.identifier.doi | 10.1016/j.isci.2024.110471 | - |
dc.identifier.pmid | 39091463 | - |
dc.identifier.isi | 001274993300001 | - |
dc.identifier.eissn | 2589-0042 | - |
local.provider.type | wosris | - |
local.description.affiliation | [Salz, Renee; 't Hoen, Peter A. C.] Radboud Univ Nijmegen, Med Ctr, Dept Med BioSci, NL-6525 GA Nijmegen, Netherlands. | - |
local.description.affiliation | [Salz, Renee; Vorsteveld, Emil E.; van der Made, Caspar I.; Kersten, Simone; Stemerdink, Merel; Riepe, Tabea, V; Mhlanga, Musa; Hoischen, Alexander; 't Hoen, Peter A. C.] Radboud Univ Nijmegen, RadboudUMC Res Inst Med Innovat, Med Ctr, NL-6525 GA Nijmegen, Netherlands. | - |
local.description.affiliation | [Vorsteveld, Emil E.; van der Made, Caspar I.; Kersten, Simone; Riepe, Tabea, V; Netea, Mihai G.; Hoischen, Alexander] Radboud Univ Nijmegen, Med Ctr, Dept Human Genet, NL-6525 GA Nijmegen, Netherlands. | - |
local.description.affiliation | [van der Made, Caspar I.; Netea, Mihai G.; Hoischen, Alexander] Radboud Univ Nijmegen, Med Ctr, Dept Internal Med, NL-6525 GA Nijmegen, Netherlands. | - |
local.description.affiliation | [van der Made, Caspar I.; Netea, Mihai G.; Hoischen, Alexander] Radboud Univ Nijmegen, Radboud Ctr Infect Dis RCI, Med Ctr, NL-6525 GA Nijmegen, Netherlands. | - |
local.description.affiliation | [Stemerdink, Merel] Radboud Univ Nijmegen, Donders Inst Brain Cognit & Behav, Med Ctr, Dept Otorhinolaryngol, NL-6525 GA Nijmegen, Netherlands. | - |
local.description.affiliation | [Volders, Pieter-Jan] Univ Ghent, Dept Biomol Med, Ghent, Belgium. | - |
local.description.affiliation | [Volders, Pieter-Jan] Jessa Hosp, Dept Clin Biol, Lab Mol Diagnost, B-3500 Hasselt, Belgium. | - |
local.description.affiliation | [Hsieh, Tsung-han; Mhlanga, Musa] Radboud Univ Nijmegen, Dept Cell Biol, NL-6500 HB Nijmegen, Netherlands. | - |
local.uhasselt.international | yes | - |
item.fulltext | With Fulltext | - |
item.contributor | Salz, Renee | - |
item.contributor | Vorsteveld, Emil E. | - |
item.contributor | van der Made, Caspar I. | - |
item.contributor | Kersten, Simone | - |
item.contributor | Stemerdink, Merel | - |
item.contributor | Riepe, Tabea, V | - |
item.contributor | Hsieh, Tsung-han | - |
item.contributor | Mhlanga, Musa | - |
item.contributor | Netea, Mihai G. | - |
item.contributor | VOLDERS, Pieter-Jan | - |
item.contributor | Hoischen, Alexander | - |
item.contributor | 't Hoen, Peter A. C. | - |
item.fullcitation | Salz, Renee; Vorsteveld, Emil E.; van der Made, Caspar I.; Kersten, Simone; Stemerdink, Merel; Riepe, Tabea, V; Hsieh, Tsung-han; Mhlanga, Musa; Netea, Mihai G.; VOLDERS, Pieter-Jan; Hoischen, Alexander & 't Hoen, Peter A. C. (2024) Multi-omic profiling of pathogen-stimulated primary immune cells. In: iScience (Cambridge), 27 (8) (Art N° 110471). | - |
item.accessRights | Open Access | - |
crisitem.journal.issn | 2589-0042 | - |
crisitem.journal.eissn | 2589-0042 | - |
Appears in Collections: | Research publications |
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File | Description | Size | Format | |
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Multi-omic profiling of pathogen-stimulated primary immune cells.pdf | Published version | 8.52 MB | Adobe PDF | View/Open |
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