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Title: | Interaction of living cable bacteria with carbon electrodes in bioelectrochemical systems | Authors: | BONNE, Robin Marshall, Ian P. G. Bjerg, Jesper J. Marzocchi, Ugo MANCA, Jean Nielsen, Lars Peter Aiyer, Kartik |
Editors: | Rudi, Knut | Issue Date: | 2024 | Publisher: | AMER SOC MICROBIOLOGY | Source: | Applied and environmental microbiology (Print), 90 (8) | Abstract: | Cable bacteria are filamentous bacteria that couple the oxidation of sulfide in sediments to the reduction of oxygen via long-distance electron transport over centimeter distances through periplasmic wires. However, the capability of cable bacteria to perform extracellular electron transfer to acceptors, such as electrodes, has remained elusive. In this study, we demonstrate that living cable bacteria actively move toward electrodes in different bioelectrochemical systems. Carbon felt and carbon fiber electrodes poised at +200 mV attracted live cable bacteria from the sediment. When the applied potential was switched off, cable bacteria retracted from the electrode. qPCR and scanning electron microscopy corroborated this finding and revealed cable bacteria in higher abundance present on the electrode surface compared with unpoised controls. These experiments raise new possibilities to study metabolism of cable bacteria and cultivate them in bioelectrochemical devices for bioelectronic applications, such as biosensing and bioremediation.IMPORTANCEExtracellular electron transfer is a metabolic function associated with electroactive bacteria wherein electrons are exchanged with external electron acceptors or donors. This feature has enabled the development of several applications, such as biosensing, carbon capture, and energy recovery. Cable bacteria are a unique class of long, filamentous microbes that perform long-distance electron transport in freshwater and marine sediments. In this study, we demonstrate the attraction of cable bacteria toward carbon electrodes and demonstrate their potential electroactivity. This finding enables electronic control and monitoring of the metabolism of cable bacteria and may, in turn, aid in the development of bioelectronic applications. Extracellular electron transfer is a metabolic function associated with electroactive bacteria wherein electrons are exchanged with external electron acceptors or donors. This feature has enabled the development of several applications, such as biosensing, carbon capture, and energy recovery. Cable bacteria are a unique class of long, filamentous microbes that perform long-distance electron transport in freshwater and marine sediments. In this study, we demonstrate the attraction of cable bacteria toward carbon electrodes and demonstrate their potential electroactivity. This finding enables electronic control and monitoring of the metabolism of cable bacteria and may, in turn, aid in the development of bioelectronic applications. | Notes: | Aiyer, K (corresponding author), Aarhus Univ, Ctr Electromicrobiol, Dept Biol, Aarhus, Denmark. kartikaiyer@bio.au.dk |
Keywords: | cable bacteria;cable bacteria;bioelectrochemical system;bioelectrochemical system;electrode;electrode;extracellular electron transfer;extracellular electron transfer;microscopy;microscopy | Document URI: | http://hdl.handle.net/1942/43656 | ISSN: | 0099-2240 | e-ISSN: | 1098-5336 | DOI: | 10.1128/aem.00795-24 | ISI #: | 001281064300001 | Rights: | 2024 Bonné et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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