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Title: | In vitro biological activities of Calamintha nepeta L. aqueous extracts | Authors: | Salhi, Nadia DELUYKER, Dorien BITO, Virginie Zaid, Abdelhamid El Rhaffari, Lhoussaine |
Issue Date: | 2024 | Publisher: | UNIV SOUTH BOHEMIA | Source: | Journal of Applied Biomedicine, p, 154-163 | Abstract: | Aim: This study aimed to investigate the phenolic composition, antioxidant capacity, and toxicity of aqueous extracts of Calamintha nepeta L. leaves and their potential vasorelaxant effects. Methods: Aqueous extracts of Calamintha nepeta L. were prepared by three extraction methods: decoction, infusion, and maceration. The total phenolic contents of the extracts and their antioxidant properties were investigated. The toxicity was evaluated by Artemia salina lethality bioassay. The decoction extract was analyzed by HPLC for its chemical profile and was also used to evaluate the vasorelaxant effect on thoracic aortic rings isolated from healthy Sprague Dawley rats. Pre-contraction was induced by phenylephrine, followed by cumulative doses of the extract (0.001 up to 250 mu g/ml). Results: Aqueous extracts of Calamintha nepeta L. showed noticeable radical scavenging and chelating activities. However, the decoction extract exhibited the most powerful antioxidant capacity. No toxicity was recorded for the extracts obtained by decoction and infusion. Caffeic acid, quercetin, and rosmarinic acid were the main identified compounds. Notably, the aqueous extract obtained by decoction induced significant relaxation in endothelium-intact aortic rings at lower concentrations, and at higher concentrations in denuded aortic rings. Conclusion: This study reveals that Calamintha nepeta L. extracted with a decoction method possesses potent antioxidant capacity and has an endothelium-dependent vasorelaxant effect. | Notes: | Salhi, N (corresponding author), Moulay Ismail Univ, Fac Sci, Lab BioActives Hlth & Environm, Meknes, Morocco. n.salhi@edu.umi.ac.ma |
Keywords: | Antioxidant activity;Antioxidant activity;Aqueous extracts;Aqueous extracts;Calamintha nepeta L.Polyphenols;Calamintha nepeta L;Polyphenols;Vasorelaxation;Vasorelaxation Abbreviations: ACh: Acetylcholine;ABTS: 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate radical scavenging assay;cGMP: Cyclic guanosine monophosphate;DPPH: 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay;dw: Dry weight;FRAP: Ferric reducing power assay;IC 50 : Concentration providing 50% inhibition of radicals;LC 50 : Lethal concentration inducing 50% lethality;LD 50 : Lethal dose inducing 50% lethality;NO: Nitric oxide;PE: Phenylephrine;ROS: Reactive oxygen species;sGC: Soluble guanylate cyclase | Document URI: | http://hdl.handle.net/1942/44448 | ISSN: | 1214-021X | e-ISSN: | 1214-0287 | DOI: | 10.32725/jab.2024.017 | ISI #: | 001308257500001 | Rights: | 2024 The Authors. Published by University of South Bohemia in České Budějovice, Faculty of Health and Social Sciences. This is an open access article under the CC BY-NC-ND license. | Category: | A1 | Type: | Journal Contribution |
Appears in Collections: | Research publications |
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