Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/45285
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dc.contributor.authorDe Vriendt, Silke-
dc.contributor.authorLaporte, Emma-
dc.contributor.authorAbayli, Berkehueuror-
dc.contributor.authorHoekx, Julie-
dc.contributor.authorHERMANS, Florian-
dc.contributor.authorLambrechts, Diether-
dc.contributor.authorVankelecom, Hugo-
dc.date.accessioned2025-02-12T08:58:40Z-
dc.date.available2025-02-12T08:58:40Z-
dc.date.issued2025-
dc.date.submitted2025-02-10T10:56:23Z-
dc.identifier.citationiScience, 28 (2) (Art N° 111708)-
dc.identifier.urihttp://hdl.handle.net/1942/45285-
dc.description.abstractThe pituitary represents the master gland governing the endocrine system. We constructed a single-cell (sc) transcriptomic atlas of male mouse endocrine pituitary by incorporating existing and new data, spanning important postnatal ages in both healthy and injured condition. We demonstrate strong applicability of this new atlas to unravel pituitary (patho)biology by focusing on its stem cells and investigating their complex identity (unveiling stem cell markers) and niche (pinpointing regulatory factors). Importantly, we functionally validated transcriptomic findings using pituitary stem cell organoids, revealing roles for Kru<euro>ppel-like transcription factor 5 (KLF5), activator protein-1 (AP-1) complex and epidermal growth factor (EGF) pathways in pituitary stem cell regulation. Our investigation substantiated changes in stem cell dynamics during aging, reinforcing the inflammatory/immune nature in elderly pituitary and stem cells. Finally, we show translatability of mouse atlas-based findings to humans, particularly regarding aging-associated profile. This pituitary sc map is a valuable tool to unravel pituitary (patho)biology.-
dc.description.sponsorshipWe thank Thomas Van Brussel, Rogier Schepers, Gino Philips, and Bram Boeckx (D.L.’s group, KU Leuven) for technical and bioinformatical support in the scRNA-seq experiments and the Genomics Core (KU Leuven), especially A´ lvaro Corte´ s Calabuig, for running the CellRanger pipeline. The ‘‘Vlaams Supercomputer Centrum’’, overseen by the Fund for Scientific Research Flanders (FWO), supplied the computational resources utilized for scRNA-seq bioinformatic analyses. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Certain figures were created using BioRender.com. This work was supported by grants from the KU Leuven Research Fund (C14/16/079; KA/20/087) and from the FWO (G0E1417N; G064723N). E.L. (11A3320N), S.D.V. (1S00823N) and B.A (11P8X24N) are supported by a PhD Fellowship from the FWO. F.H. was supported by an FWO project grant (G061819FWO).-
dc.language.isoen-
dc.publisherCELL PRESS-
dc.rights2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).-
dc.titleSingle-cell transcriptome atlas of male mouse pituitary across postnatal life its stem cell landscape-
dc.typeJournal Contribution-
dc.identifier.issue2-
dc.identifier.volume28-
local.format.pages20-
local.bibliographicCitation.jcatA1-
dc.description.notesVankelecom, H (corresponding author), Katholieke Univ Leuven, Dept Dev & Regenerat, Lab Tissue Plast Hlth & Dis, Cluster Stem Cell & Dev Biol, B-3000 Leuven, Belgium.-
dc.description.noteshugo.vankelecom@kuleuven.be-
local.publisher.place50 HAMPSHIRE ST, FLOOR 5, CAMBRIDGE, MA 02139 USA-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr111708-
dc.identifier.doi10.1016/j.isci.2024.111708-
dc.identifier.isi001403261200001-
local.provider.typewosris-
local.description.affiliation[De Vriendt, Silke; Laporte, Emma; Abayli, Berkehueuror; Hoekx, Julie; Vankelecom, Hugo] Katholieke Univ Leuven, Dept Dev & Regenerat, Lab Tissue Plast Hlth & Dis, Cluster Stem Cell & Dev Biol, B-3000 Leuven, Belgium.-
local.description.affiliation[Hermans, Florian] Hasselt Univ, Biomed Res Inst BIOMED, Fac Med & Life Sci, Dept Cardiol & Organ Syst, B-3590 Diepenbeek, Belgium.-
local.description.affiliation[Lambrechts, Diether] Katholieke Univ Leuven, Dept Human Genet, Lab Translat Genet, B-3000 Leuven, Belgium.-
local.description.affiliation[Lambrechts, Diether] Vlaams Inst Biotechnol, Ctr Canc Biol VIB, B-3000 Leuven, Belgium.-
local.uhasselt.internationalno-
item.fulltextWith Fulltext-
item.contributorDe Vriendt, Silke-
item.contributorLaporte, Emma-
item.contributorAbayli, Berkehueuror-
item.contributorHoekx, Julie-
item.contributorHERMANS, Florian-
item.contributorLambrechts, Diether-
item.contributorVankelecom, Hugo-
item.fullcitationDe Vriendt, Silke; Laporte, Emma; Abayli, Berkehueuror; Hoekx, Julie; HERMANS, Florian; Lambrechts, Diether & Vankelecom, Hugo (2025) Single-cell transcriptome atlas of male mouse pituitary across postnatal life its stem cell landscape. In: iScience, 28 (2) (Art N° 111708).-
item.accessRightsOpen Access-
crisitem.journal.eissn2589-0042-
Appears in Collections:Research publications
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