Electric Polarization-Dependent Absorption and Photocurrent Generation in Limnospira indica Immobilized on Boron-Doped Diamond

Abstract

We present the change of light absorption of cyanobacteria in response to externally applied electrical polarization. Specifically, we studied the relation between electrical polarization and changes in light absorbance for a biophotoelectrode assembly comprising boron-doped diamond as semiconducting electrode and live Limnospira indicaPCC 8005 trichomes embedded in either polysaccharide (agar) or conductive conjugated polymer (PEDOT-PSS) matrices. Our study involves the monitoring of cyanobacterial absorbance and the measurement of photocurrents at varying wavelengths of illumination for switched electric fields, i.e., using the bioelectrode either as an anode or as cathode. We observed changes in the absorbance characteristics, indicating a direct causal relationship between electrical polarization and absorbing properties of L. indica. Our finding opens up a potential avenue for optimization of the performance of biophotovoltaic devices through controlled polarization. Furthermore, our results provide fundamental insights into the wavelength-dependent behavior of a bio photovoltaic system using live cyanobacteria.