Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/47671
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dc.contributor.authorHERMANS, Florian-
dc.contributor.authorVankelecom, Hugo-
dc.contributor.authorBRONCKAERS, Annelies-
dc.contributor.authorLAMBRICHTS, Ivo-
dc.date.accessioned2025-11-03T14:38:01Z-
dc.date.available2025-11-03T14:38:01Z-
dc.date.issued2025-
dc.date.submitted2025-10-23T09:04:25Z-
dc.identifier.citationTurksen, Kursad (Ed.). Methods in Molecular Biology: Organoids: Stem Cells, Structure, and Function, Springer Nature,-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/1942/47671-
dc.description.abstractOrganoid models are a powerful 3D stem cell technology to explore tissue (patho-)biology and development. Tissue-derived (i.e., from tissue biopsies) organoids are long-term and stably expandable while more closely recapitulating key phenotypical and functional characteristics of the tissue-of-origin than traditional 2D culture systems. Additionally, organoids can differentiate into tissue-specific cell types, for instance, following exposure to defined differentiation cues. Although prevailing in vitro cell models have deepened our understanding of mouse tooth development and biology, in vitro representations of the dental epithelium lack (the combination of) these benefits of tissue-derived organoids and are at most derived from one tooth type. Here, we describe the protocol to establish, propagate, and differentiate mouse tooth organoids from both early postnatal molar and incisor teeth. The established organoids display a dental epithelial stemness phenotype and acquire a maturation-stage ameloblast-like phenotype following differentiation.-
dc.description.sponsorshipThis work was supported by the Fund for Scientific Research (FWO) Flanders (FWO grant G061819FWO to I.L.; FWO postdoctoral fellowship 1226325N to F.H.).-
dc.language.isoen-
dc.publisherSpringer Nature-
dc.relation.ispartofseriesMethods in Molecular Biology-
dc.rightsThe copyright in the Contribution shall be vested in the name of the Author. The Author has asserted their right(s) to be identified as the originator of the Contribution in all editions and versions, published in all forms and media. The Author agrees that all editing, alterations or amendments to the Contribution made by or on behalf of the Publisher or its licensees for the purpose of fulfilling this Agreement or as otherwise allowed by the above rights shall not require the approval of the Author and will not infringe the Author's "moral rights" (or any equivalent rights). This includes changes made in the course of dealing with retractions or other legal issues.-
dc.subject.otherAmeloblast-
dc.subject.otherDental epithelium-
dc.subject.otherIncisors-
dc.subject.otherMolars-
dc.subject.otherStem cells-
dc.subject.otherTooth development-
dc.titleEstablishment of 3D Tooth Organoid Culture from Early-Postnatal Mouse Molar and Incisor-
dc.typeBook Section-
dc.relation.edition2-
local.bibliographicCitation.authorsTurksen, Kursad-
local.format.pages17-
local.bibliographicCitation.jcatB2-
dc.relation.references[1] Sato T, Vries RG, Snippert HJ, Van De Wetering M, Barker N, Stange DE, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 2009;459:262–5. https://doi.org/10.1038/nature07935. [2] Clevers H. Modeling Development and Disease with Organoids. Cell 2016;165:1586–97. https://doi.org/10.1016/j.cell.2016.05.082. [3] Artegiani B, Clevers H. Use and application of 3D-organoid technology. Hum Mol Genet 2018;27:R99–107. https://doi.org/10.3389/fcell.2021.671475. [4] Hemeryck L, Hermans F, Chappell J, Kobayashi H, Lambrechts D, Lambrichts I, et al. Organoids from human tooth showing epithelial stemness phenotype and differentiation potential. Cellular and Molecular Life Sciences 2022;79:153. https://doi.org/10.1007/s00018-022-04183-8. [5] Hermans F, Hemeryck L, Bueds C, Torres Pereiro M, Hasevoets S, Kobayashi H, et al. Organoids from mouse molar and incisor as new tools to study tooth-specific biology and development. Stem Cell Reports 2023;18:1166–81. https://doi.org/10.1016/j.stemcr.2023.03.011. [6] Kim HY, Cooley V, Kim EJ, Li S, Lee JM, Sheyfer D, et al. Adult dental epithelial stem cell-derived organoids deposit hydroxylapatite biomineral. Int J Oral Sci 2023;15:55. https://doi.org/10.1038/s41368-023-00257-w. [7] Hermans F, Hasevoets S, Vankelecom H, Bronckaers A, Lambrichts I. From Pluripotent Stem Cells to Organoids and Bioprinting: Recent Advances in Dental Epithelium and Ameloblast Models to Study Tooth Biology and Regeneration. Stem Cell Rev Rep 2024;Epub ahead of print. https://doi.org/10.1007/S12015-024-10702-W. [8] Naveau A, Zhang B, Meng B, Sutherland MT, Prochazkova M, Wen T, et al. Isl1 controls patterning and mineralization of enamel in the continuously renewing mouse incisor. Journal of Bone and Mineral Research 2017;32:2219–31. https://doi.org/10.1002/jbmr.3202. [9] Rhodes JA, Fitzgibbon DH, Macchiarulo PA, Murphy RA. Epidermal growth factor-induced precocious incisor eruption is associated with decreased tooth size. Dev Biol 1987;121:247–52. https://doi.org/10.1016/0012-1606(87)90156-4. [10] Cielinski MJ, Jolie M, Wise GE, Marks SC. The contrasting effects of colony-stimulating factor-1 and epidermal growth factor on tooth eruption in the rat. Connect Tissue Res 1995;32:165–9. https://doi.org/10.3109/03008209509013720. [11] Ganss B, Abbarin N. Maturation and beyond: proteins in the developmental continuum from enamel epithelium to junctional epithelium. Front Physiol 2014;5:371. https://doi.org/10.3389/fphys.2014.00371. [12] Welborn VV. Enamel synthesis explained. Proc Natl Acad Sci U S A 2020;117:21847–8. https://doi.org/10.1073/pnas.2014394117. [13] Collignon AM, Castillo-Dali G, Gomez E, Guilbert T, Lesieur J, Nicoletti A, et al. Mouse Wnt1-CRE-RosaTomato Dental Pulp Stem Cells Directly Contribute to the Calvarial Bone Regeneration Process. Stem Cells 2019;37:701–11. https://doi.org/10.1002/stem.2973.-
local.type.refereedNon-Refereed-
local.type.specifiedBook Section-
local.bibliographicCitation.statusIn press-
dc.identifier.doi10.1007/7651_2025_623-
dc.identifier.pmid40106152-
dc.identifier.eissn1940-6029-
local.provider.typePubMed-
local.bibliographicCitation.btitleMethods in Molecular Biology: Organoids: Stem Cells, Structure, and Function-
local.uhasselt.internationalno-
item.contributorHERMANS, Florian-
item.contributorVankelecom, Hugo-
item.contributorBRONCKAERS, Annelies-
item.contributorLAMBRICHTS, Ivo-
item.fullcitationHERMANS, Florian; Vankelecom, Hugo; BRONCKAERS, Annelies & LAMBRICHTS, Ivo (2025) Establishment of 3D Tooth Organoid Culture from Early-Postnatal Mouse Molar and Incisor. In: Turksen, Kursad (Ed.). Methods in Molecular Biology: Organoids: Stem Cells, Structure, and Function, Springer Nature,.-
item.accessRightsRestricted Access-
item.fulltextWith Fulltext-
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