Please use this identifier to cite or link to this item: http://hdl.handle.net/1942/48926
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dc.contributor.authorVerleye, Kaat-
dc.contributor.authorNoppen, Sam-
dc.contributor.authorBoonen, Arnaud-
dc.contributor.authorYildizhan, Yagmur-
dc.contributor.authorVan Loy, Tom-
dc.contributor.authorHeens, Cindy-
dc.contributor.authorVanderhoydonc, Frank-
dc.contributor.authorPinheiro, Claudio-
dc.contributor.authorPINCELA LINS, Paula-
dc.contributor.authorBRONCKAERS, Annelies-
dc.contributor.authorHendrix, An-
dc.contributor.authorSwinnen, Johannes V.-
dc.contributor.authorSpasic, Dragana-
dc.contributor.authorLammertyn, Jeroen-
dc.contributor.authorPannecouque, Christophe-
dc.contributor.authorSchols, Dominique-
dc.date.accessioned2026-04-17T07:55:00Z-
dc.date.available2026-04-17T07:55:00Z-
dc.date.issued2026-
dc.date.submitted2026-04-07T11:32:53Z-
dc.identifier.citationBiosensors, 16 (3) (Art N° 174)-
dc.identifier.urihttp://hdl.handle.net/1942/48926-
dc.description.abstractThis study leverages surface plasmon resonance (SPR) BiacoreTM technology to unveil the diagnostic potential of detecting CXCR4 on extracellular vesicles (EVs). Despite its recognized potential as a cancer biomarker, the presence of CXCR4 on EVs remains underexplored for diagnostic purposes. Using reference material (rEVs), a standardized label-free and real-time SPR biosensor is established to molecularly profile CXCR4-positive EVs. The binding interactions between immobilized antibodies and EVs isolated from different cancer cell lines revealed a unique SPR molecular fingerprint (SPR-MFP) consisting of varying expression levels of the CD9, CD63 and CD81 EV biomarkers, as well as CXCR4. There was a strong correlation between CXCR4 expression on the cellular membrane measured by flow cytometry (FCM) and the CXCR4 SPR signal of purified EVs, indicating that the chemokine receptor is actively transferred to the extracellular space. The BiacoreTM biosensor is able to directly detect and molecularly profile EVs in buffer and spiked in cell culture supernatant supplemented with 10% EV-depleted serum. Altogether, our findings illuminate the potential of SPR BiacoreTM technology in EV-related research as well as reveal the diagnostic potential of EV-associated CXCR4, offering valuable insights and paving the way for medical applications in diseases associated with aberrant CXCR4 expression.-
dc.description.sponsorshipFunding: TEM microscopy was funded by Research Foundation Flanders (FWO) grant number I000220N Acknowledgments: This work was supported by HIV Virology and Chemotherapy royalties. We thank Erik Martens for technical assistance during SEC preparation. Nathan Vanalken, Robin Hermans, Daisy Ceusters, Sandra Claes and Geert Schoofs are acknowledged for their excellent technical assistance and scientific advice. We further acknowledge Nanoview for access to and demonstration of the Exoview R200. Finally, we acknowledge the Advanced Optical Microscopy Centre at Hasselt University for support with the microscopy experiments.-
dc.language.isoen-
dc.publisherMDPI-
dc.rights2026 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.-
dc.subject.othersurface plasmon resonance-
dc.subject.otherbiosensor-
dc.subject.otherextracellular vesicles-
dc.subject.otherCXCR4-
dc.subject.otherbiomarker-
dc.titleSurface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles-
dc.typeJournal Contribution-
dc.identifier.issue3-
dc.identifier.volume16-
local.format.pages22-
local.bibliographicCitation.jcatA1-
dc.description.notesVerleye, K; Schols, D (corresponding author), Katholieke Univ Leuven, Mol Struct & Translat Virol Rega Inst, Dept Microbiol Immunol & Transplantat, B-3000 Leuven, Belgium.-
dc.description.noteskaat.verleye@kuleuven.be; sam.noppen@kuleuven.be;-
dc.description.notesyagmur.yildizhan@tss.ox.ac.uk; tom.vanloy@kuleuven.be;-
dc.description.notescindy.heens@kuleuven.be; frank.vanderhoydonc@kuleuven.be;-
dc.description.notesclaudio.pinheiro@ugent.be; paula.pincelalins@uhasselt.be;-
dc.description.notesannelies.bronckaers@uhasselt.be; an.hendrix@ugent.be;-
dc.description.notesj.swinnen@kuleuven.be; dragana.spasic@kuleuven.be;-
dc.description.notesjeroen.lammertyn@kuleuven.be; christophe.pannecouque@kuleuven.be;-
dc.description.notesdominique.schols@kuleuven.be-
local.publisher.placeMDPI AG, Grosspeteranlage 5, CH-4052 BASEL, SWITZERLAND-
local.type.refereedRefereed-
local.type.specifiedArticle-
local.bibliographicCitation.artnr174-
dc.identifier.doi10.3390/bios16030174-
dc.identifier.isiWOS:001725800900001-
local.provider.typewosris-
local.description.affiliation[Verleye, Kaat; Noppen, Sam; Boonen, Arnaud; Yildizhan, Yagmur; Van Loy, Tom; Schols, Dominique] Katholieke Univ Leuven, Mol Struct & Translat Virol Rega Inst, Dept Microbiol Immunol & Transplantat, B-3000 Leuven, Belgium.-
local.description.affiliation[Yildizhan, Yagmur; Spasic, Dragana; Lammertyn, Jeroen] Katholieke Univ Leuven, Dept Biosyst, Biosensors Grp, B-3000 Leuven, Belgium.-
local.description.affiliation[Heens, Cindy; Pannecouque, Christophe] Katholieke Univ Leuven, Mol Genet & Therapeut Virol & Oncol Rega Inst, Dept Microbiol Immunol & Transplantat, B-3000 Leuven, Belgium.-
local.description.affiliation[Vanderhoydonc, Frank; Swinnen, Johannes V.] Katholieke Univ Leuven, Dept Oncol, Lab Lipid Metab & Canc, B-3000 Leuven, Belgium.-
local.description.affiliation[Pinheiro, Claudio; Hendrix, An] Univ Ghent, Dept Human Struct & Repair, Lab Expt Canc Res, B-9000 Ghent, Belgium.-
local.description.affiliation[Lins, Paula M. Pincela; Bronckaers, Annelies] Hasselt Univ, Biomed Res Inst, Dept Cardio & Organ Syst COS, B-3590 Diepenbeek, Belgium.-
local.uhasselt.internationalno-
item.accessRightsOpen Access-
item.contributorVerleye, Kaat-
item.contributorNoppen, Sam-
item.contributorBoonen, Arnaud-
item.contributorYildizhan, Yagmur-
item.contributorVan Loy, Tom-
item.contributorHeens, Cindy-
item.contributorVanderhoydonc, Frank-
item.contributorPinheiro, Claudio-
item.contributorPINCELA LINS, Paula-
item.contributorBRONCKAERS, Annelies-
item.contributorHendrix, An-
item.contributorSwinnen, Johannes V.-
item.contributorSpasic, Dragana-
item.contributorLammertyn, Jeroen-
item.contributorPannecouque, Christophe-
item.contributorSchols, Dominique-
item.fullcitationVerleye, Kaat; Noppen, Sam; Boonen, Arnaud; Yildizhan, Yagmur; Van Loy, Tom; Heens, Cindy; Vanderhoydonc, Frank; Pinheiro, Claudio; PINCELA LINS, Paula; BRONCKAERS, Annelies; Hendrix, An; Swinnen, Johannes V.; Spasic, Dragana; Lammertyn, Jeroen; Pannecouque, Christophe & Schols, Dominique (2026) Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles. In: Biosensors, 16 (3) (Art N° 174).-
item.fulltextWith Fulltext-
crisitem.journal.eissn2079-6374-
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